Not listed THIS PROJECT ADDRESSES STANDARD PROGRAM AREA 4: ENVIRONMENTAL EFFECTS OF GE RELATIVE TO NON-GE ORGANISMS IN THE CONTEXT OF PRODUCTION SYSTEMS. OUR FOCUS IS ON PROGRAM AREA 4D AS THIS PROJECT IS A COMPARATIVE ASSESSMENT OF ENVIRONMENTAL IMPACTS OF AGRICULTURAL PRODUCTION SYSTEMS USING ORGANIC AND/OR CONVENTIONAL METHODS WITH THOSE INVOLVING PLANT, ANIMAL, OR MICROBIAL BIOTECHNOLOGY. HERE GENETICALLY ENGINEERED TOMATO PLANTS ARE COMPARED WITH NON-GENETICALLY ENGINEERED TOMATO MUTANSTHAT ALL HAVE INCREASED IMMUNITY FROM PLANT PATHOGENS OUR GOAL IS TO IDENTIFY ENVIRONMENTAL HAZARDS ASSOCIATED WITH THE GENETICALLY ENGINEERED LINES. TOMATO LINES WILL BE CULTURED UNDER AGRICULTURAL CONDITIONS WITH FOUR PLANTINGS OVER TWO YEARS. IN OBJECTIVE 1, PLANT AND SOIL MICROBIOME CHANGES WILL BE DETERMINED IN FIELD-GROWN GENETICALLY ENGINEEREDLINES IN THE FIELD COMPARED TO THE OTHER LINES. MICROBIOME ANALYSIS WILL INCLUDE FULL-LENGTH RRNA OPERON AND SHOTGUN METAGENOMIC SEQUENCING. IN OBJECTIVE 2, THE EFFECTS OF GE LINES ON DISEASE PRESSURE, SYMPTOM RATINGS, YIELD, HORTICULTURAL CHARACTERISTICS, SOIL NUTRIENTS, AND BENEFICIAL MICROBIAL POPULATIONS WILL BE MEASURED. IN OBJECTIVE 3, ALL THE DATA FROM OBJECTIVES 1 AND 2 WILL BE INTEGRATED TO LEARN THE MOST FROM THE DATA FOR REGULATORY DECISION MAKING.OUR HYPOTHESIS IS THAT THE ENVIRONMENTAL EFFECTS OF THE GENETICALY ENGINEEREDLINES WILL BE MINIMALCOMPARED TO THE STANDARD TOMATO LINE USED FOR PRODUCTION. $0 6/20/25 Not listed **AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** HAZARD COMPARISONS, UNDER FIELD CONDITIONS, OF GE-DERIVED, CRISPR-MUTANT, AND WILD-TYPE TOMATO LINES THAT VARY IN PLANT DEFENSE EXPRESSION $500.0k 8/12/22