Synthesis and Cloning of 101 & 218 Individual Genes

DESCRIPTION: The National Institutes of Health - NICHD intends to procure the services of a contractor to provide synthesis and Cloning of 101 & 218 Individual Genes to determine the binding of various mouse Znf alleles alone and to each other and when co-expressed with mPRDM9.  There are 319 different but highly repetitive codon optimized mouse znf arrays cloned into an expression vector.

 Lab’s rationale for sole source:

 “We study how the epigenetic state of mammals is initially established in early development, by exploring KRAB-zfp transcription factors that recognize parasitic mobile DNA elements to initiate stable heterochromatic silencing. We also study how one large family of these parasitic elements, the endogenous retroviruses or ERVs, play an important role in development and evolution of new traits in mammals by rewiring gene expression networks.  Finally, we explore how the epigenetic state of a cell works in conjunction with combinatorial codes of transcription factors to generate the huge diversity of cell types required for mammalian development.

These constructs will be used to elucidate how these transcription factors interact with chromatin and regulate the expression of genes, by expressing in cells and then doing ChIP-seq to look at binding properties.

This company has previously synthesized all the ZFP arrays used in our labs prior and current ongoing experiments. Changing variables in the middle of the project/experiments would compromise the integrity of the previous data collected.  These newly synthesized arrays would be used in conjunction with the ones this company has previously produced to maintain continuity of the projects in the lab.

All the SC1692 VectorArk Cloning is free of charge instead of $49.00 each, saving thousands for the Lab which we will not receive from any other vendor, assuming that all cloning being equal

This proposed procurement is for a service for which the government intends to negotiate with one source, Genscript USA, Inc located in Piscataway, NJ.   In order to comply with the requirements in this Special Notice, the responses should be based on our interpretation of the general requirements provided.  Interested persons may identify their interest and capability to respond to the requirement or submit quotations. This notice is not a request for competitive quotes. However, all quotes received within the time and date of publication of this "Special Notice” will be considered by the Government. A determination by the Government not to compete with this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a future competitive procurement.

Required supplies and Services:

SC1843 Gene Synthesis:2010315B03Rik_J3QK55 Length: 1110bp,

SC1843 Gene Synthesis:2410141K09Rik_K7N769  Length: 1203bp and

434 other Gene synthesis for different lengths. 

HOW TO RESPOND: In order to compete for this project, interested parties must be registered in the Central Contracting Registration/SAM and submit a price quotation by 10:00 pm, ET, July  23rd  2020. Offerors should submit one copy of the quote to MOE FILALI via email. Electronic transmissions to Moe.Filali@nih.gov are preferable but must be timely. It is the responsibility of the Offeror to ensure the proposal is received by the date and time shown above.

The following Federal Acquisition Regulations clauses are incorporated by reference: FAR 52.212-3, Offeror Representations and Certifications-Commercial Items (Mar 2005), 52.212-4 (Sept 2005), 52.212-5, Contract Terms and Conditions Required to Implement Statutes of Executive Orders-Commercial Items (Aug 2006). (6)(i) 52.222-26, Equal Opportunity (Apr 2002) (E.O. 11246). (18) 52.222-35, Equal Opportunity for Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (19) 52.222-36, Affirmative Action for Workers with Disabilities (Jun 1998) (29 U.S.C. 793). (20) 52.222-37, Employment Reports on Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (31) 52.232-33, Payment by Electronic Funds Transfer-Central Contractor Registration (Oct 2003) (31 U.S.C. 3332).

Primary Point of Contact:

MOE FILALI Contracting Office Address:
 

NIH/NICHD Office of Acquisitions

6710B Rockledge Drive, Room 1145D

Bethesda, MD 20817

(w) 301.827 7737

Moe.filali@nih.gov