eDNA Sequencing Services
Closed Solicitation Posted
This opportunity was awarded. See the award notice from .
- Solicitation number
- W9132T25Q0011
- Agency
-
US Army Corps of Engineers
Department of the Army, Department of Defense
- Responses due
- Set-aside
- No set-aside
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- Points of contact
-
- Andrea Thomas cerl-ct-quotes@usace.army.mil (217) 373-4479
Notice details come from SAM.gov. Updated .
About this opportunity
The US Army Corps of Engineers' Engineer Research and Development Center - Construction Engineering Research Laboratory (ERDC-CERL) is seeking DNA sequencing services for environmental DNA (eDNA) metabarcoding projects focused on surveying endangered, at-risk, and invasive species on military lands. The contractor will provide comprehensive sequencing support, including consultation on sequencing strategy, library quality control, DNA sequencing, demultiplexing of sequence libraries, and data interpretation assistance. Vendors must demonstrate extensive experience with eDNA samples, including annual metabarcoding throughput, client history, operational longevity, and detailed technical capabilities such as available sequencing platforms and contamination control procedures. The evaluation will primarily assess the vendor's relevant eDNA experience and price reasonableness, with quotes due by February 28, 2025, at 11:59 AM CDT. Interested contractors must be registered in the System for Award Management (SAM) and submit their proposals via email to CERL-CT-Quotes@usace.army.mil.
The solicitation is unrestricted, with no specific set-aside designations, and falls under NAICS code 541380 (Testing Laboratories and Services) with a size standard of $19 million. The contract will involve sequencing eight data libraries using the Illumina NovaSeq X Plus platform, with an expected total data output of 24TB across 8 lanes and a 12-month performance period. While no current incumbent is explicitly named, the project requires targeted libraries focusing on specific metabarcoding markers like CO1, 12S, and ITS2. The place of performance will involve returning processed libraries to 2902 Newmark Drive, Champaign, IL 61822, with the government providing shipping labels. The scope of work emphasizes technical precision, including fragment analysis, qPCR quality checks, and the ability to troubleshoot suboptimal sequencing results, with a requirement to provide sequencing results within one month of library delivery.
Notice text
2 versions
Update #2 · Latest ·
Amendment 1: This amendment is issued to extend the solicitation closing date.
_______________________________________________________________________________________________________
The Engineer Research and Development Center – Construction Engineering Research Laboratory (ERDC-CERL) is leading several projects that utilize molecular tools, specifically environmental DNA (eDNA), to survey for endangered, at-risk and invasive species and whole community biodiversity on military and related lands. ERDC-CERL seeks support for DNA sequencing of DNA metabarcoding samples, to include quality control and data interpretation. ERDC-CERL will be responsible for sample collection and preparation of sequence libraries (adaptors ligated and indexed). The Contractor shall provide consultation for determining optimal sequencing strategy/platform and quality controls for prepared libraries prior to sequencing, perform library quality control, determine optimized library loading concentration, DNA sequencing, and demultiplexing of sequence libraries, and assist with data interpretation and troubleshooting.
The objective of this effort is to derive DNA sequence data for a suite of environmental DNA projects that survey for endangered, at-risk and invasive species and whole community biodiversity on military and related lands. The Contractor shall provide consultation for determining optimal sequencing strategy/platform and quality controls for prepared libraries prior to sequencing, perform library quality control, DNA sequencing, and demultiplexing of sequence libraries, and assist with data interpretation and troubleshooting. The resulting data will be incorporated into reporting to installation and DoD sponsors regarding distribution of focal species on military installations.
Please reference the attached written RFQ for full detail of the requirement as well as all applicable provisions and clauses.
The quote shall include the information required in FAR clause 52.212-1(b). In addition, quoters shall provide sufficient information for the Government to determine its level of confidence in the ability of the Quoter to satisfy the requirements of the RFQ based on an assessment of relevant DNA Sequencing and Data Interpretation for Environmental DNA experience. This may include
- Annual metabarcoding throughput of the quoters business
- Experience working with eDNA samples, including the number of clients, how long the operation has been in business, total runs per year, and targeted loci used in the past
- Full list of available sequencing and quality control platforms and expected capabilities in the future
- Full list of contamination precautions and controls used throughout the sequencing process to ensure no contamination.
- any other related technical data
Please provide responses to this notice no later than the date and time listed above to:
CERL-CT-Quotes@usace.army.mil
Telephone responses will not be accepted.
* Note: in order to be eligible for award of a Government contract, vendors must be actively registered in the System for Award Management (SAM), have no active exclusions, and a complete representations and certifications. This system combines data that was formerly contained in the Central Contractors Registration (CCR), Online Representation and Certifications (ORCA), and the Excluded Parties List System (EPLS). For more information, review the SAM website at https://www.sam.gov.
Update #1 ·
The Engineer Research and Development Center – Construction Engineering Research Laboratory (ERDC-CERL) is leading several projects that utilize molecular tools, specifically environmental DNA (eDNA), to survey for endangered, at-risk and invasive species and whole community biodiversity on military and related lands. ERDC-CERL seeks support for DNA sequencing of DNA metabarcoding samples, to include quality control and data interpretation. ERDC-CERL will be responsible for sample collection and preparation of sequence libraries (adaptors ligated and indexed). The Contractor shall provide consultation for determining optimal sequencing strategy/platform and quality controls for prepared libraries prior to sequencing, perform library quality control, determine optimized library loading concentration, DNA sequencing, and demultiplexing of sequence libraries, and assist with data interpretation and troubleshooting.
The objective of this effort is to derive DNA sequence data for a suite of environmental DNA projects that survey for endangered, at-risk and invasive species and whole community biodiversity on military and related lands. The Contractor shall provide consultation for determining optimal sequencing strategy/platform and quality controls for prepared libraries prior to sequencing, perform library quality control, DNA sequencing, and demultiplexing of sequence libraries, and assist with data interpretation and troubleshooting. The resulting data will be incorporated into reporting to installation and DoD sponsors regarding distribution of focal species on military installations.
Please reference the attached written RFQ for full detail of the requirement as well as all applicable provisions and clauses.
The quote shall include the information required in FAR clause 52.212-1(b). In addition, quoters shall provide sufficient information for the Government to determine its level of confidence in the ability of the Quoter to satisfy the requirements of the RFQ based on an assessment of relevant DNA Sequencing and Data Interpretation for Environmental DNA experience. This may include
- Annual metabarcoding throughput of the quoters business
- Experience working with eDNA samples, including the number of clients, how long the operation has been in business, total runs per year, and targeted loci used in the past
- Full list of available sequencing and quality control platforms and expected capabilities in the future
- Full list of contamination precautions and controls used throughout the sequencing process to ensure no contamination.
- any other related technical data
Please provide responses to this notice no later than the date and time listed above to:
CERL-CT-Quotes@usace.army.mil
Telephone responses will not be accepted.
* Note: in order to be eligible for award of a Government contract, vendors must be actively registered in the System for Award Management (SAM), have no active exclusions, and a complete representations and certifications. This system combines data that was formerly contained in the Central Contractors Registration (CCR), Online Representation and Certifications (ORCA), and the Excluded Parties List System (EPLS). For more information, review the SAM website at https://www.sam.gov.
Files
| File | Type | Posted |
|---|---|---|
| B08.02 Questions and Answers 1-6.docx | DOCX document | |
| B08.02 Questions and Answers 1-5.docx | DOCX document | |
| B08.02 Solicitaiton W9132T25Q0011 Amendment 01.pdf | ||
| B08.02 Questions and Answers 1.docx | DOCX document | |
| B08.02 Solicitaiton W9132T25Q0011.pdf |
Notice history
| Notice | Type | Posted |
|---|---|---|
| eDNA Sequencing Services | Award Notice | |
| This notice | Solicitation |
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