Exhibit_A-_Price_Sheet.xlsx

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Histological and Immunohistological Services
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W81XWH-18-R-0027
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Exhibit A - Price Sheet

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Sheet1

Unit PriceTotal Price (Ceiling)
TitleDescriptionMaximum QuantityBase YearOption 1Option 2Option 3Option 4Base YearOption 1Option 2Option 3Option 4
Silver StainingDetection of neurodegeneration with FD NeuroSilver™ Kit II in brains. Price per brain.200
Cryostat Section StainingStaining of cryostat sections (40 μm) of the brain with fluoro-jade B. Price per slice.150
Brain Prep for StainingPreparation and mounting previously cut free-floating cryostat sections of the brain on Superfrost Plus or gelatin-coated microscope slides (2 sections per slide) for routine or special stain. Price per section, including the cost of slides.210
Tissue Preparation - CerebrumTissue preparation (cerebrum) including cryoprotection, freezing, sectioning with a cryostat (interval: 480 μm, 22 sections per set, 12 sets per brain), mounting one set of sections on Superfrost plus slides, collection and storage of 5 sets of free-floating sections in FD section storage solution™ at -20ºC for up to 3 years*. Price per cerebrum.200
Tissue Preparation - BrainsTissue preparation (brains) including cryoprotection, freezing, sectioning with a cryostat (sagittal or horizontal sections, collection and storage of 5 sets of free-floating sections in FD section storage solution™ at -20ºC for up to 3 years*. Price per brain.200
Tissue Preparation - Cerebrum ATissue preparation (cerebrum) including cryoprotection, freezing, sectioning with a cryostat (interval: 960 μm, 12 sections per set, 24 sets per brain), mounting one set of sections on Superfrost plus slides, collection and storage of 5 sets of free-floating sections in FD section storage solution™ at -20ºC for up to 3 years*. Price per cerebrum *Continued storage of tissue after 6 months (frozen brains) or 3 years (unstained sections) will be subject to a storage fee ($1.00 per brain per year).200
Tissue Preparation - Cerebrum BTissue preparation (cerebrum) including cryoprotection, freezing, sectioning with a cryostat (interval: 240 μm, 44 sections per set, 6 sets per brain), mounting one set of sections on Superfrost plus slides, collection and storage
of 5 sets of free-floating sections in FD section storage solution™ at -20ºC for up to 3 years*. Price per cerebrum *Continued storage of tissue after 6 months (frozen brains) or 3 years (unstained sections) will be subject to a storage fee ($1.00 per brain per year).150
Tissue Preparation - Olfactory BulbsTissue preparation (olfactory bulbs) including cryoprotection, freezing, sectioning with a cryostat, collection and storage of 3 sets of free-floating sections in FD section storage solution™ at -20ºC for up to 3 years*. Price per pair of olfactory.200
Brain EmbeddingEmbedding of the brain blocks in paraffin. Price for up to 10 blocks200
Tissue Preparation - Peripheral OrgansTissue preparation (peripheral organs) for paraffin embedding. Price per per organ.150
Peripheral Organ EmbeddingEmbedding of peripheral organ in paraffin. Price for up to 10 blocks.300
Tissue Preparation - Brains for BlockerTissue preparation (brains) for paraffin embedding, including blocking each brain through specific levels using a brain blocker. Price per brain.300
Peripheral Organ Microtome CutsCut sections from paraffin-embedded peripheral organs with a microtome, including paraffin block preparation and optimizing cutting conditions. Price per block.300
Peripheral Organ MountingMount paraffin-embedded sections from peripheral organs on Superfrost Plus microscope slides (2 sections per slide). Price per slide (including slide cost).300
Cryostat Sectioning - Brainstem and CerebellumCut cryostat sections through brainstem and cerebellum, including mounting one set of sections on Superfrost plus slides, collection and storage of 5 sets of free-floating sections in FD section storage solution™ at -20ºC for up to 3 years*. Price per section.300
Mounting - Sagitally Cut SectionsMount sagittally cut free-floating cryostat sections on Superfrost Plus slides (2 sections per slide), including slide cost. Price per section.249
Mounting - CoronaSurcharge for mounting unstained or stained free-floating sections (coronal) of brains on microscope slides. Price per section.253
Mounting - BrainSurcharge for mounting sagittally cut free-floating cryostat sections (unstained or stained) of brains on slides. Price per section.251
Sectioning - Fresh Frozen BrainsSectioning of unfixed fresh-frozen brains with a cryostat. Price per brain.250
Mounting - Unfixed Coronal SectionsMount unfixed coronal sections of the brain on Superfrost. Price per section. Price per section (60 % discount) *Continued storage of tissue after 6 months (frozen brains) or 3 years (unstained sections) will be subject to a storage fee ($1.00 per brain per year). Plus microscope slides (2 sections per slide), including the cost of slides. Price per section.250
Selection - Cryostat SectionsSelection of free-floating cryostat sections through specific regions (levels) from previously cut brains. Price per section.250
Cutting - Brain SectionsCut coronal or sagittal sections from paraffin-embedded brain blocks with a microtome, including paraffin block preparation and optimizing cutting conditions. Price per block.150
Mounting - Brains on Superfrost PlusMount paraffin-embedded sections of the brains on Superfrost Plus microscope slides (2 sections per slide). Price per slide (including slide cost).150
Staining - Brain SectionsStaining of cryostat sections of the brains with FD hematoxylin & eosin solution™. Price per slide.150
Staining - Brain Sections AStaining of paraffin-embedded sections of brains with hematoxylin & eosin. Price per slide.150
Staining - Peripheral OrgansStaining of paraffin-embedded sections from peripheral organs with FD hematoxylin & eosin solution™. Price per slide.150
Counterstaining - BrainCounterstaining of immunofluorescence-labeled sections of the brain with DAPI. Price per section.150
Colorimetric Immunostaining AColorimetric immunostaining with 1 primary antibody on free-floating sections of the brain (interval: 960 μm, 12 sections per brain). Price per brain.150
Colorimetric Immunostaining BColorimetric immunostaining with 1 primary antibody on free-floating sections of the brain (interval: 240 μm, 44 sections per brain). Price per brain.150
Colorimetric Immunostaining CColorimetric immunostaining with 1 primary antibody on free-floating sections of the brain. Price per brain.150
Colorimetric Immunostaining DColorimetric immunostaining with 1 primary antibody on sagittally cut free-floating sections of the brain (interval: 480 μm, approximately 30 sections per brain). Price per brain.150
Colorimetric Immunostaining EColorimetric immunostaining with 1 primary antibody on free-floating sections of the brainstem and cerebellum. Price per section.150
Colorimetric Immunostaining - Olfactory BulbsColorimetric immunostaining with 1 primary antibody on free-floating sections from olfactory bulbs (approximately 34 sections per olfactory bulb). Price per pair of olfactory bulbs.150
Colorimetric Immunostaining FColorimetric immunostaining with 1 primary antibody on paraffin embedded sections of the brain. Price per slide (2 sections per slide).150
Colorimetric Immunostaining GColorimetric immunostaining with 1 primary antibody on sections mounted on slides (fresh-frozen sections). Price per slide.150
Immunofluorescence - BrainImmunofluorescence labeling with 1 primary antibody on free-floating sections of the brain. Price per brain.150
Immunofluorescence - Brainstem and CerebellumImmunofluorescence labeling with 1 primary antibody on free-floating sections of the brainstem and cerebellum. Price per section.150
Immunofluorescence 2Immunofluorescence labeling with 2 primary antibodies on free-floating sections of the brain. Price per brain.175
Immunofluorescence 2AImmunofluorescence labeling with 2 primary antibodies on free-floating sections of the brain. Price per section brain.170
Immunofluorescnce 2BImmunofluorescence labeling with 2 primary antibodies on sections mounted on slides (fresh-frozen sections of brains). Price Per slide.190
Antibody Testing & TitrationTesting & titration of the primary antibody for colorimetric immunostaining on free-floating sections from the brain. Price per antibody.80
Antibody Testing & Titration ATesting & titration of the primary antibody for colorimetric immunostaining on sections mounted on slides. Price per antibody.225
Antibody Testing & Titration BTesting & titration of the primary antibody for immunofluorescence labeling on free-floating sections of the brain. Price per antibody.200
Antibody Testing & Titration CTesting & titration of the primary antibody for immunofluorescence labeling on brain sections mounted on slides. Price per antibody.175
Golgi-stainingGolgi-staining of brains with FD Rapid Golgistain™ kit. Price per brain.250
Staining - Timm's sulphide silverTimm’s sulphide silver staining of the brain. Price per slide.250
Tissue pick upTissue pick up. Price per pick up.250
FormaldeydePhosphate-buffered 4% paraformaldehyde, 1L bottle. Price per bottle.250
SlidesSlide box (hold 100 slides). Price per box.150
Total9,628

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