SB1341-15-RQ-0730-001_SOW.pdf

STATEMENT OF WORK

TITLE: To purchase a gas chromatograph (GC) with a triple quadrupole mass spectrometric (MS) detector, equipped with an additional MS inlet allowing direct probe access to the MS source.

I. BACKGROUND INFORMATION

The Chemical Sciences Division (CSD), part of NIST's Material Measurement Laboratory, serves as the Nation's reference laboratory for chemical measurements and standards to enhance U.S.

industry's productivity and competitiveness, assure equity in trade, and provide quality assurance for chemical measurements used for assessing and improving public health, safety, and the environment. The CSD is responsible for development and characterization of Standard Reference Materials (SRMs) to enhance chemical measurement comparability within the U.S. and to provide a basis for measurement comparability with other nations. SRMs are provided to support clinical, food, environmental, fuel, metal alloy, cement, and advanced materials analyses. Research activities are geared toward improvement of measurement capabilities to address U.S. industry and government agencies' requirements for new measurement services and reference materials.

One of the tools available to the CSD for the characterization of SRMs and other complex samples is the gas chromatograph/mass spectrometer (GC/MS). Through its use of 15 m to 100 m long fused capillary columns, this instrument enables the separation of complex natural mixtures. In addition the mass spectrometer enables selective detection of specific target analytes as well as qualitative screening for non-targeted species. This type of instrument has been used for the characterization of SRMs including SRM 1950 Metabolites in Human Plasma, SRM 2779 Gulf of Mexico Oil, SRM 2378 Fatty Acids in Human Serum, SRM 3671 Nicotine Metabolites in Human Urine, SRM 3672 Organic Contaminants in Smokers’ Urine and SRM 3672 Organic Contaminants in Non-Smokers’ Urine. We currently have six GC/MS instruments in the Organic Chemical Measurement Science Group, and all are single quadrupole systems, suitable for routine to moderately challenging sample analyses. A gas chromatograph (GC) equipped with a triple quadrupole mass spectrometer (MS), currently not available in our laboratory, would enable more selective detection of targeted organic compounds in highly complex sample matrices including environmental (such as soils, sediments, and atmospheric dust) and clinical (serum and urine).

II. SCOPE OF WORK

The purpose of this procurement is to obtain a gas chromatograph/triple quadrupole mass spectrometer with source pressure readout (ion gauge or comparable), capable of both EI and positive and negative chemical ionization (CI). The system shall be equipped with both a GC and a direct probe interface, a liquid autosampler and a computer with associated instrument control, data acquisition and data analysis software. The GC will be equipped with both cool on-column and split/splitless injectors. The vendor shall install this system, provide training, a one year warranty, and maintenance and repairs for the entire system for 3 option years after the expiration of the warranty.

III. SPECIFICATIONS

The system shall meet or exceed the following minimum specifications:

1. Physical characteristics and instrument specifications:

a. The instrument shall be of a bench-top design (≤2 ft 5 in deep and ≤4 ft wide).

b. The instrument shall include a high capacity autosampler supporting ≥ 100 liquid samples at 1.5 mL each in a single unattended measurement. The sample introduction system shall be capable of injecting 0.2 to 5 µL into an on-column or a split/splitless injector.

c. The GC oven should be capable of sub-ambient temperatures of -40 °C to a maximum temperature of 450 °C and the two injectors (on-column and split/splitless) should be capable of tracking the temperature of the GC oven.

d. The MS shall have separate and permanently affixed GC and direct probe interfaces, be capable of electron ionization (EI), positive chemical ionization (PCI), and negative chemical ionization (NCI), and have a scan range of from m/z 20 to 1000, with a scan rate of > 15000 amu/sec.

e. The instrument shall include a computer, monitor, instrument control and operation software to perform independent precursor ion scan, product ion scan, and neutral gain scan. For elaboration, a neutral gain scan yields a spectrum formed of all precursor ions that have undergone a selected m/z increase corresponding to the mass of a particular neutral species divided by the charge number of the precursor. The ions detected are the product ions produced by gain of a preselected neutral species mass following ion/molecule reactions of the precursor ions with a reactant gas in a collision cell. The computer used for instrument control and data acquisition shall be new OEM equipment, with a Microsoft Windows 7 operating system. The system shall include an i7 processor or faster, at least 16 Gb RAM, six or more USB ports, a 2 Tb hard drive, and a licensed copy of Office 365. The computer system shall also consist of one

(1) 24-inch monitor with a minimum resolution of 1680 X 1050 dpi. The computer system and monitor shall not consist of any refurbished materials. Data reporting (e.g., integrated peak areas) shall be exportable to Microsoft Excel for subsequent processing.

f. The contractor shall provide one (1) electronic copy in PDF format of the operations and maintenance manual covering proper operation, routine maintenance, and trouble shooting for the instrument and controlling software to the NIST Technical Point of Contact (TPOC).

2. Minimum performance specifications for triple quadrupole mass spectrometer The instrument shall meet the minimum performance specifications in Table 1.

Table 1. Minimum Performance Criteria

Specification Criteria Value Sensitivity

Injected mass of octafluoronaphthalene (OFN), methyl stearate, and/or benzophenone in electron ionization (EI), positive chemical ionization (PCI), or negative chemical ionization (NCI).

≤ 1 pg, OFN, mass/charge (m/z) 272 signal/noise (s/n)

≥ 1,500:1 (EI Scan) ≤ 100 fg OFN, m/z 272 → 222, s/n ≥

8000:1, multiple reaction monitoring

(MRM), EI.

≤ 100 fg benzophenonen-d10, m/z 193 → 110, s/n ≥ 200:1, MRM, PCI.

≤ 10 fg OFN, m/z 272, s/n ≥ 400:1, NCI SIM.

Scanning Atomic mass units/sec (amu/s), step resolution.

Scan cycles

≥15000 amu/s, ≤ 0.15 amu/step

≥ 315 scans/s

Resolution Atomic mass unit ≤0.2 amu, peak width half-height

Mass Axis stability

±amu over 2-day period ±0.15 amu

Installation:

The Contractor shall install the instrument in Building 227, Room A126 at NIST Gaithersburg, MD. Installation shall include, at a minimum, uncrating/unpacking of all equipment, set-up and hook-up of all equipment, start-up, demonstration of specifications, and removal of all trash.

Instrument will operate to the required specifications upon installation. Installation will take place during normal business hours, between 8:30 am and 5:00 pm Eastern Time, Monday through Friday except Federal Holidays, and will be coordinated with the NIST Technical Point of Contact (TPOC). Installation is to occur within 90 days of award. An installation report in a hard copy format including data confirming that the instrument has met the minimum specifications and minimum performance criteria shall be provided to the NIST TPOC upon completion of installation. Installation shall occur within 30 days of delivery of the system.

Training:

The Contractor shall provide training at NIST for up to 5 NIST personnel covering normal operation, troubleshooting, and routine maintenance. Training will be provided during normal business hours, between 8:30am and 5:00pm Eastern Time, Monday through Friday, except Federal Holidays, and will be coordinated with the NIST TPOC to ensure maximum availability of NIST personnel. Training shall occur within 30 days of installation and acceptance.

Warranty:

The Contractor shall provide a one year warranty for the system. All hardware, consumables for normal operation of the system and software shall be covered. The warranty shall cover all parts, labor, and travel. Only OEM certified parts shall be used in the repair and maintenance of the instrument. The warranty shall commence upon successful completion of delivery, installation, training and demonstration of all required specifications. During the warranty period, the Contractor shall perform two preventative maintenance services that include cleaning or replacement of parts as needed and verification that the system is operating within the minimum performance criteria (see Table 1, above).

During the warranty period, the Contractor shall provide telephone support during normal business hours, and on-site service and replacement of any defective equipment or software. The Contractor must respond to NIST personnel not later than two hours after receiving a request for service or technical support. The Contractor must provide their best effort to be on-site at NIST within two business days of the initial request for service.

For each service request under the warranty period, the Contractor shall provide a written service report detailing the time of arrival at NIST, all services that were performed, documentation showing that the equipment is operating at the required specifications, and time of departure from NIST. The detailed service report shall be provided to the TPOC upon completion of the service request.

Maintenance Services (OPTION LINE ITEMS):

The Contractor shall perform maintenance services and repairs for the system at the expiration of the warranty period for up to three (3) one-year option periods. During each maintenance period all hardware, consumables for normal operation of the system and software shall be covered as well as all parts, labor and travel. Additionally, the Contractor shall provide telephone support during normal business hours and on-site service and replacement of any defective equipment or software. The Contractor must respond to NIST personnel not later than two hours after receiving a request for service or technical support. The Contractor must provide their best effort to be on-site at NIST within two business days of the initial request for service.

Two preventive maintenance visits per year shall be included during each of the three maintenance service years in which Original Equipment Manufacturer (OEM) trained and certified service personnel shall verify the proper operation and calibration of the system.

For all maintenance visits, the Contractor shall provide a written service report detailing the time of arrival at NIST, all services that were performed, documentation showing that the equipment is operating at the required specifications, and time of departure from NIST. The detailed service report shall be given to the NIST TPOC upon completion of the service request.

IV. PLACE OF PERFORMANCE

Installation, training, maintenance and repair will be at NIST, 100 Bureau Dr, Gaithersburg, MD 20899 except when mutually agreed upon by both parties. Normal business hours are Monday through Friday, 8:30 a.m. to 5:00 p.m. with the exception of Federal holidays.

V. ACCEPTANCE

Acceptance of the system shall occur after successful installation and documentation in the installation report showing that the system is working to the minimum required specifications has been accepted by the NIST Contracting Officer’s Representative (COR).

VI. DELIVERABLES

Deliverable Description Quantity Due Date GC-triple quadrupole EI and CI capable MS and source pressure measurement (ion gauge or comparable), with cool on-column and split/splitless injectors equipped GC and direct probe interfaces.

One (1) System

60 days ARO

Operations and Maintenance Manual in electronic PDF format shall be provided to the TPOC upon delivery of the system.

One (1) Due upon delivery of the system.

Installation report in a hard copy format and verification that required specifications are met by measurement of suitable analytes for all GC inlets and direct probe inlet and with EI and CI detection. The installation report shall be given to the TPOC upon completion of installation. Acceptance of the system will be provided at the conclusion of the installation and acceptance of the installation report by the NIST COR.

One (1) 30 days after delivery of the equipment

Deliverable Description Quantity Due Date Training of up to 5 NIST personnel covering normal operation, troubleshooting, and routine maintenance.

One (1) 30 days after Installation and Acceptance.

Warranty Services. Two annual preventive maintenance visits per year, unlimited repair visits, consumables, hardware and software coverage, travel and labor.

One (1) Year

Commences at time of acceptance

(OPTIONAL) Maintenance services. Two annual preventive maintenance visits per year, unlimited repair visits, consumables, hardware and software coverage, travel and labor.

Three (3) Years

Commence at the expiration of each previous period.

VII. GENERAL INFORMATION

Safety: The Contractor employee shall be responsible for knowing and complying with all installation safety prevention regulations. Such regulations include, but are not limited to, general safety, fire prevention, and waste disposal.

Security: NIST is a restricted campus. An identification badge is required for access for entry into buildings and also is shown to the armed Security Police when entering the campus.

Regular Business Hours. Regular business hours are Monday through Friday, 8:00 am to 5:00 pm Eastern Time.