SS_Attachment_I_thru_V.pdf

PDF 418 KB Posted Dec 10, 2015

Attached to: Glassware and Media Processing Services
Solicitation number: NIHES2016059
Issued by: Department of Health and Human Services National Institutes of Health

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Attachments I thru V

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SF30.pdf	PDF	Apr 19, 2016
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NIHES2014059-RFP.pdf	PDF	Mar 31, 2016
SOW_MediaandGlasswareServices.pdf	PDF	Mar 31, 2016

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Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 4700 813 968 4 74 720 November 12600 1166 1378 1400 239 440 December 13800 2712 1400 205 248 360 January 12500 1593 1773 200 134 9 February 12600 2080 1493 200 195 760 March 19000 1160 2099 130 252 1080 April 17200 320 1761 180 249 940 May 12300 1130 2232 354 183 1160 June 15700 1160 1814 200 352 1300 July 15700 609 2232 200 296 1160 August 15800 1160 2491 1200 249 320 September 13300 928 2406 140 253 928 Total 165,200 14,831 22,047 4,413 2,724 9,177

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 3270 214 586 100 40 720 November 11648 239 379 100 125 320 December 6312 125 216 80 145 January 8900 110 308 80 132 680 February 8716 616 162 110 89 2104

March 9040 508 625 180 180 1342 April 7231 512 464 120 143 360 May 6702 414 1395 100 116 1342 June 10656 363 556 120 163 1088 July 10879 464 666 100 152 1632

August 6680 1058 323 140 244 200 September 10557 508 467 120 115 792

Total 100,591 5,131 6,147 1,350 1,644 10,580

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 6382 162 509 80 4 720 November 4317 262 531 120 125 440 December 6498 720 1220 180 145 360 January 13449 343 855 80 132 3736 February 8008 256 573 80 89 1000

March 5412 465 765 180 180 1704 April 7814 180 356 120 143 720 May 4845 622 922 100 116 2616 June 9530 496 816 120 163 1960 July 9198 609 1153 100 152 1342

August 13300 928 1203 80 250 1984 September 15800 1160 1731 20 252 1203

Total 104,553 6,203 10,634 1,260 1,751 17,785

FY 2013

FY 2014

FY 2015

Attachment II: Media Production, FY 2013-FY 2015

NIHES2016059 Sources Sought Attachment II

Attachment III: Standard Order Media Products

# 0 Minimal Medium Plates

#0 / MINIMAL MEDIUM PLATES w/kan.25ug/ml

#07/ MINIMAL MEDIUM PLATES W/LACTOSE

#21 MINIMAL MEDIUM WITH IPTG (red/brown)

#4 LB PLATES (LURIA AGAR PLATES) <BTL>

#4 LB PLATES (LURIA AGAR PLATES) <DISH>

#45 LB + STRIPTOMYCIN cc: yellow-green

#5 P-GAL MEDIUM (color: green)

#54 LB + RIF color code: green-yellow

#60 MINIMAL MEDIA CC: BLUE,BLACK

#65 MM - PLATES cc: blue_green

#66 MM - PLATES (color code:blue-blue)

#69 Minimal Agar

(MEM) ALPHA MEDIUM

1.5% AGAR

10% EDTA DECALIFATION of BONE

10% NEUTRAL BUFFERED FORMALIN

10X PBS Ca-Mg Free

10X TBE

10X TRIS BUFFERED SALINE

13x100 tubes

1M MgCL2 SOLUTION

20 X SSC

2X YT MEDIA

50X TAE BUFFER

50X VB Medium Conc.

5X TBE

NIHES2016059 Sources Sought Atttachment III https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

ACK LYSIS BUFFER (GROVES)

ALG #2 (LEU DROP W/2% GALACTOSE

alternative recipe for versene

BAP (TRYPTOCASE SOY AGAR)

BG PLATES

BHI = BOVINE HEART INFUSION AGAR PLATES

brain heart infusion medium

BREWER'S THIOGLYCOLLATE BROYH

BRUCILLA AGAR W/TVP (CULTURE TUBE)

BRUCILLA AGAR W/TVP (PETRI DISHES)

Can Agar w/o URA

CAN PLATES W/O LEU

CAN PLATES W/O TRP.

CAN,GAL-COMPLETE PLATES

CDO -URA -TRP SCGL Recipe

CDO -TRP SCGL Recipe

CDO YPGL Recipe

CHA1 PLATES

CHA2 PLATES w/o ADENINE

CHOCOLATE AGAR PLATES

Chocolate Agar with IsoVitalex Plate

Columbia Blood Agar

COM AGAR W/O TRP,URA, & LEU

COM AGAR W/O URA and TRP W/ SORB (UTTOP)

COM PLATES W/O HIS and LEU.

COM PLATES W/O LYS and LEU.

COM PLATES W/O URA and LEU.

COM PLATES W/O TRP-LEU

COM PLATES W/O TRP-LEU-HIS

Com w/o trp plates

Com-Trp- & Ura Plates

COMP MIN DROPOUT PLATE W/O URACIL (btl)

COMP MIN DROPOUT PLATE W/O URACIL (dish)

DMEM (high glucose) GIBCO # 11965118

DMEM LOW GLUCOSE GIBCO #11885076

ENT PLATES (M-ENTEROCOCCUS AGAR)

G418-YPDA

GAL - URA PLATES

GAL- TRYP.PLATES

GAL-LYSINE PLATES

GAL-PE-LEU.PLATES

GLUTAMAX-1 SUPPLEMENT

GRAM NEGATIVE BROTH (GN)

H 40 PLATES

H-40 X PLATES

HAM'S F-10 nutrient mix. gibco # 81200

HAM'S F-12 GIBCO CAT 11765062

Hank's Balance Salt Solution w/ Ca & Mg

HANK'S BALANCED SALT SOLUTION-CMF

HEPES BALANCD SALT SOLUTION W/PENN-STREP

HEPES BUFFER

HG AGAR = HIGH GROWTH PLATES

Hyg-YPDA

ISCOVE'S MODIFIED DULBECCO'S (IMDM)

Isotonic Hepes

K-AGAR PLATES

K-agar plates (100 mm)

K-AGAR PLATES (60mm)

L-AGAR PLATES and BROTH LOW SODIUM (dsh)

L-AGAR PLATES W/KANAMYCIN

LB (LURIA-BERTANI) MEDIUM)

LB + KANAMYCIN (cc: #17 brown-purple)

LB + TETRACYCLINE (15 ug/ml)

LB + TETRACYCLINE (15 ug/ml) + AMP

LB AGAR W/AMP PLATES

LB Agar w/Chloramphenical,25ug/ml

LB AGAR WITH AMPICILLIN (50 UG/ML) DISH

LB AGAR WITH AMPICILLIN + TETRACYCLINE

LB AGAR/TETRACYCLINE W/15 ug/ml

LB SOFT AGAR

LB W/AMP PLATES COLOR CODE - #47

LB W/TETRACYCLINE 15 ug/ml Medium #15

LB WITH KANAMYCIN AND X-GAL

LB/AMP/X-GAL/IPTG* PLATES

LB/KAN/X-GAL + IPTG

LB/TET/AMP/X-GAL/IPTG* PLATES

LCTG MEDIA PLATES CODE #63

LITMUS MILK

Low salt LB Agar with Zeocin 25mg/liter

LYSINE IRON AGAR (LIA) SLANTS DEEP

M-9 Plates w/Thiamine

M9 Medium

MAC PLATES

Malt Extract Agar

MANNITOL SALT AGAR

MAR # 11 MET AGAR PLATES

MAR # 12 PHE AGAR PLATES

MAR # 13 SER AGAR PLATES (brown-orange)

MAR # 14 THR AGAR PLATES (brown/yellow)

MAR # 16 TYR AGAR PLATES color-cd:yellow

MAR # 18 VAL AGAR PLATES

MAR # 20 CAN AGAR PLATES

MAR # 4 ARG AGAR PLATES

MAR # 5- ASP AGAR PLATES

MAR # 6 GLU AGAR PLATES color-code:BLUE

MAR # 9. LEU AGAR PLATES

MAR #10 LYS AGAR PLATES

MAR #15 TRP AGAR PLATES (brown/green)

MAR #17: URA AGAR PLATES (brown/purple)

MAR #1:MIN AGAR PL and BROTH (PTR DISH)

MAR #22 CAN-FOA AGAR PLATES

MAR #22 CAN-FOA AGAR Plates

MAR #23:CAN-low_ADE AGAR PLATES

MAR #24:C0M-low_ADE AGAR PLATES

MAR #2: C0M AGAR PLATES Color/Code: red

MAR #2: COM AGAR PLATES

MAR #3: ADE AGAR PLATES

MAR #7: HIS AGAR PLATES colr code:purple

McCOY'S 5A MEDIUM W/O PENN and STREP

MEM (GIBCO # 10370021

MEM Medium gibco# 11400(equiv.to#11385

MEM w/ EARLES SALTS w/o L-GLUTAMINE

MEM W/EARLE'S SALTS W/O GLUTAMINE

MEM w/o glutamine (Sigma# M3024) minimal non inducing media

MR/VP BROTH

MSA = MANNITOL SALT AGAR PLATES

MYCOSEL AGAR

NEMATODE AXENIC MEDIUM

NGH BROTH = NG W/O AGAR

NGM AGAR= NEMATODE GROWTH MEDIUM AGAR

NZY BROTH

Oocyte Ringer 2 (OR2)

OPTI-MEM GIBCO CAT#31985-070

PBS - CMF

PBS CMF (Gibco / sheri)

PBS with Ca & Mg

PBS-CMF w/o KCL

PBS-CMF W/SODIUM AZIDE

PHENOL RED BROTH W/1% ADONITAL

POTATO DEXTROSE AGAR (TUBE)

POTATO DEXTROSE W/P-S CYCLOHEXAMIDE

Powdered COM-sugar-hallut

PS Gal w/o Leu & Lys

PS Gal W/O TRP & LEU

PS Gal w/o Trp,Leu, & Lys.

PS Gal w/o Trp,Leu,& His

PSEUDOMONAS/POUR PLATES

PSEUDOMONAS/POUR TUBES

Puck's Saline "A"

RPMI 1640 Rice

RPMI GIBCO #21870 W/O L-GLUTAMINE

RPMI-1640 (GIBCO CAT # 11875119

RPMI-1640 w/o Phenol Red ,Cat# 13200

RYM #36: BC PLATES

RYM #37: BC-TRP PLATES

RYM 13: SP3-22 AGAR PLATES

RYM 14: MIN AGAR PLATES W/ URA and LEU

RYM 15/C: GAL-C0M AGAR PLATES

RYM 1: YPD BROTH

RYM 26: LEU AGAR PLATES

RYM 26: LEU AGAR PLATES W/SORBITOL

RYM 26: LEU AGAR W/SORBITOL (LTOP)

RYM 31: TRP BROTH

RYM 3: YPDA AGAR PLATES

RYM 5: YPG AGAR PLATES

S S AGAR PLATES

S.O.C. MEDIA

S.O.C. MEDIUM

SABOURAUD DEXTROSE AGAR (PETRI DISH)

SABOURAUD DEXTROSE AGAR (TUBES)

SABOURAUD DEXTROSE AGAR SLANTS

SCGL Broth

SD SER #1

SD-URA PLATES

SDS PAGE BUFFER (10X)

Selinite

SOB BROTH

SOFT AGAR (13 X 100 TUBES)

SOFT AGAR (KIMBLE BOTTLES)

STARCH AGAR

Sterile Culture tube 13x100 mm sterile culture tubes

Sterile Toothpicks

TB1 Agar, bottle (Wilson)

TB1 Agar, plates (Wilson)

TB1 PLATES and TOP AGAROSE (PETRI DISH)

TB1 Top Agar, bottle (Wilson)

TERRIFIC BROTH

TERRIFIC BROTH w/o glycerol

TETRAT BROTH W/0.001% BRILLIANT GREEN

Todd Hewitt Broth

TRANSFER BUFFER (tris glycine)

TRANSPORT MEDIUM

Triton Lysis Buffer

TRP AGAR PLATES EX- AD and HIS

TRYPTONE GLUCOSE EXTRACT AGAR

TSA = TRYPTIC SOY AGAR PLATES

TSI SLANTS

VERSENE

VIOLET RED AGAR

Whitten's + HEPES Medium

Whitten's Midium

Williams

Williams 'E' Medium w/P &S

WILLIAMS' MEDIUM 'E' HAM'S F-12 1:1 MIX

YAS-1: YPDA BROTH

YES (Yeast Extract with Supplements)

YNB

YP-Gal Broth

YP-GAL PLATES

YPD + Zeocin

YPD AGAR PLATES

YPD BROTH

YT AGAR PLATES

YT MEDIA

ZP 5FOA Plates

ZP 5FOA(R/G) Plates

ZP SC(R/G)-URA

ZP YP(R/G)A BROTH

ZP YP(R/G)A PLATES

NIHES2016059 Sources Sought Atttachment III https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

SS Attachment II.pdf

Sheet1

SS Attachment III.pdf

Sheet1

File details come from the government source that posted it. Updated Nov 14, 2017.