SOW_MediaandGlasswareServices.pdf

PDF 449 KB Posted Mar 31, 2016

Attached to: Glassware and Media Processing Services
Solicitation number: NIHES2016059
Issued by: Department of Health and Human Services National Institutes of Health

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RFP NIEHS2016059 Statement of Work

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SF30.pdf	PDF	Apr 19, 2016
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Amendment_1.pdf	PDF	Apr 5, 2016
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SS_Attachment_I_thru_V.pdf	PDF	Dec 10, 2015

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Glassware and Media Acquisition Plan Attachment I

STATEMENT OF WORK (SOW)

CENTRALIZED GLASSWARE AND MEDIA PROCESSING

SERVICES

I. Introduction and Background

The National Institute of Environmental Health Sciences (NIEHS), located in the Research Triangle Park, North Carolina (RTP), is one of the 27 Institutes and centers composing the National Institutes of Health (NIH). The mission of NIEHS is dedicated to reducing the burden of diseases and dysfunctions associated with the environment.

This mission is accomplished by conducting basic and applied research into the effects of environmental exposures on biological systems and human health; on the identification of susceptible human subpopulations; and on the interaction between the environment, genetics and age.

II. Scope and Requirements

A. Objective

The purpose of this effort is to provide on-site personnel for the preparation of biological media, tissue cultures, and “buffers” as well as washing, sterilizing, stocking, issuance, and collection of glassware as listed in Attachment I.

B. General Requirements

Independently, and not as an agent of the Government, the Contractor shall furnish all necessary services and qualified technical personnel, not otherwise provided by the Government to provide preparation of biological media, tissue cultures, and “buffers”, as well as washing, sterilizing, issuance, and collection of glassware as required at the NIEHS in RTP. Additionally, the Contractor shall provide restocking service for disposable plastic ware and service to prepare sterile custom media using Government-furnished chemicals and media supplies.

C. Contractor Requirements

The Contractor shall provide trained personnel with experience in preparation of sterile custom media and sterile laboratory glassware. All workmanship performed by the Contractor shall be of acceptable industry standard quality and shall be immediately rectified at no additional cost to the Government upon notification of non-acceptability by the Government. In addition, the Contractor shall observe commercially accepted safety and precautionary procedures pertaining to handling of laboratory equipment and biological and chemical materials. The Contractor shall provide and require its employees to use appropriate personal protective equipment (i.e. safety glasses, uniforms, safety shoes, respiratory protection, etc.) as provided by the Government.

The Contractor shall be responsible for safeguarding all Government property (as listed in Attachment II) that will be provided for use by the Contractor under this contract. The Contractor shall be held responsible for the cost of any repairs caused by negligence and abuse on their part. All Contract personnel must observe all regulations in effect at NIEHS.

D. Government Furnished Equipment, Facilities, and Supplies.

a. The Government will provide all equipment, facilities, laboratory space, office space, materials and supplies needed for the Contractor to provide the required services. The Government Contracting Officer’s Representative (COR) will place orders for all Government-furnished supplies and equipment.

b. Security: The COR will ensure that service personnel have recognizable identification badges to gain access to the NIEHS facility in accordance with NIEHS/NIH/HHS policy and procedure.

E. Reporting Requirements and Deliverables

Reports – The contractor shall track media orders and glassware usage monthly.

Monthly reports detailing media orders and glassware usage shall be provided electronically in Microsoft Word or Excel 2007 or compatible versions to the COR by the tenth day of the following month. The contractor shall also maintain and document supplies, chemicals and glassware inventories. Lists of needed supplies shall be provided to the COR on a weekly basis or as needed.

The Contractor must provide monthly usage reports and occasionally some service reports to the COR on an ad-hoc basis as requested.

F. Definitions

The definitions listed below are the special terms and phrases used in this SOW or elsewhere in this contract document. For the purpose of the contract, all definitions will apply throughout.

1. Autoclave – An apparatus (machine) that sterilizes using live steam at high pressure and high temperatures.

2. Contamination/Contaminated – Items that have been in contact with potentially hazardous chemicals, radioisotopes, or biological materials.

3. Disposable Glassware – Non-reusable container made of glass.

4. Disposable Plastic – Non-reusable container made of plastic.

5. Fly Media Bottles – Glass bottles that contain media used to propagate colonies of flies.

6. Glassware – Any reusable container that is made of glass.

7. Hazardous Material/Hazardous Waste – Chemicals, radioisotopes, or biological materials with the potential for causing harmful biological effects either acutely or chronically in humans or animals.

8. Medium/Media – A substance/mixture in or on which organisms are cultivated or grown for scientific purposes or study.

9. MOATS – Web-based Media Ordering and Tracking System developed by the Government.

10. Quality Assurance Plan (QAP) - The plan, developed by the Government specifically for this contract, to assure compliance with the contract.

11. Quality Control (QC) - Those actions taken by the Contractor to control the quality of services so that they meet the requirements of this contract.

12. Quality Control Plan (QCP) - The plan, developed by the Contractor specifically for this contract, to assure compliance with the performance work statement.

13. Special Orders – Written request for specific type(s) or quantities of glassware or other miscellaneous items.

14. Sterilize – The destruction of all microorganisms by combination of physical or chemical means.

G. Specific Requirements and Tasks/Deliverables

The Contractor shall provide the following services:

A. Delivery and Pickup Services – The Contractor shall collect dirty glassware from the laboratory areas in Building 101, NIEHS South Campus at a minimum of twice per day, morning and afternoon. All collections and deliveries shall be made between the hours of 8:30 am and 5:00 pm. In addition, the Contractor shall deliver custom orders of media to the laboratory areas as requested by the scientific personnel. The Contractor shall also collect any unused portion or contaminated media from the laboratory areas in concert with the collection of used glassware.

B. Cleaning – Dirty glassware, new glassware, fly media bottles and vials, wash trays, pots, velvet cloth, caps, and tubes shall be washed, inspected, packaged, and stocked.

A description of the types of glassware and approximate monthly totals cleaned in FY13, FY14 and FY15 (October 2012 – September 2015) has been provided as Attachment III. The Contract staff shall also provide routine cleaning of the work area to include table tops, equipment, carts, floors, sinks, and general work area.

C. Stocking – Glassware, disposable items, chemicals, etc. will be delivered to the glassware and media area from the NIEHS warehouse and other outside sources. The Contractor shall receive, unpack, wash and/or stock all items and dispose of any debris in the dumpsters located in the Module E loading dock.

D. Media Preparation – The Contractor shall prepare and label complex sterile custom biological culture media and drosophila growth media as requested by the scientific staff. Requests for media are typically received throughout the day on a daily basis. A description of the types of media in various size containers and approximate monthly totals prepared in FY13, FY14 and FY15 (October 2012 – September 2015) has been provided as Attachment IV. Standard and custom media orders are submitted from the laboratories using the web-based Media Ordering and Tracking System (MOATS). A copy of the Standard Order Entry form has been provided as Attachment VII. In addition, a list of standard order media products has been provided as Attachment V. Several examples of standard media recipes from the previous list are provided as Attachment VI.

E. Quality Assurance – The contract personnel shall collect samples from all batches of custom media as prepared. Contract personnel shall place a sample in an incubator for observation. Another sample shall be submitted to the NIEHS QA lab for sterility tests. Results from the sterility tests are typically received by the COR from the NIEHS QA lab within 24 hours. In the event that contamination is detected, the entire batch will immediately be retrieved from the lab and a new batch prepared, tested and delivered to the requestor. In addition to these QA steps required by the NIEHS, the Contractor shall develop a written QCP which includes schedules, methods and results of actions taken by the Contractor to control the quality of goods or services to meet the requirements of the SOW.

III. Quality Assurance, Inspection and Acceptance Criteria

The Government will monitor the Contractor performance under this contract and shall retain the right to perform unscheduled inspections, as it deems necessary.

Furthermore, the Government retains all rights provided by the Federal Acquisition Regulations (FAR) clause entitled Inspection of Services Fixed Price and any other inspection clause, which may be specified in the contract.

The COR and/or the Alternate COR will monitor the Contractor’s activities and accomplishments and will indicate acceptance or non-acceptance of the deliverables.

The deliverables identified above will be inspected and either approved or, if they are not acceptable, rejected by the COR and/or the Alternate COR. Such inspections will occur as determined to be necessary by the COR and/or Alternate COR.

Quality Assurance Plan Desired Outputs

Deliverable Performance Indicator

Acceptable Quality Level

Monitoring Method

Pickup and Delivery

Daily glassware and media pickup and delivery

Completion and timeliness of pickups and deliveries

95% Conformance

Random COR Inspection and Customer Concerns

Cleaning Providing safe and efficient cleaning and sterilization service

Availability of glassware as needed by the scientific staff

98% Conformance

Random COR Inspection and Customer Concerns

Stocking Providing routine stocking service

Availability of glassware and disposable items

95% Conformance

Random COR Inspection and Customer Concerns

Media Preparation

Preparation of standard media orders

Media is prepared within the time specified on the MOATS order, according to the recipe, free from contamination

98% Conformance

Random COR Inspection and Customer Concerns;

NIEHS Quality Assurance testing

Quality Assurance

Adherence to the Quality Control Plan

Service and products provided according to the Contractor QCP and Government

QAP

98% Conformance

Random COR Inspection and Customer Concerns;

NIEHS Quality Assurance testing

Reporting Monthly Usage Reports

Prompt and accurate Service Reports

95% Conformance

Receipt of reports by COR or Alternate PO

Attachment I: Government Owned Glassware and Pastic Items

Glass Items Plasic Items

Beakers Flasks 30ml Tissue Culture Flask 50ml 100ml Filters 150ml 115ml 250ml 150ml 400ml 500ml 600ml 1000ml 1000ml Tissue Culture Filters 2000ml 4000ml Trays

Animal Trays Cylinders 10ml Petri Dishes 25ml 60X15mm 50ml 100X15mm 100ml 150X25mm 250ml 500ml Caps for Tubes 1000ml 13X100 Caps 2000ml 20X150 Caps

Cylinders Carboys w/stoppers 10ml 25ml 50ml 100ml 250ml 500ml 1000ml 2000ml

Bellco Flasks 25ml 60ml 125ml 250ml 500ml 1000ml

Erlenmeyer Flasks 25ml 50ml 125ml 250ml 500ml 1000ml 2000ml 4000ml

Filtering Flasks 25ml 50ml 125ml 250ml 500ml 1000ml 2000ml 4000ml

Volumetric Flasks 10ml 25ml 50ml 100ml 200ml 250ml 500ml 1000ml 2000ml 4000ml

Erlenmeyer Flasks w/stoppers 25ml 50ml 125ml 250ml 500ml 1000ml

Kimble Bottle 100ml

Kimble Bottles w/small mouth 100ml 500ml 1000ml

Specimen Bottles 16ml 6ml

Reagent Bottles 500ml 1000ml 2000ml 4000ml

Solution Bottles Wide Mouth Big Wide Mouth Small Small Mouth

Jugs

Fern Back Flask

Jars 16 oz 16oz wide mouth

Tubes 6X50mm Durham Tubes 13X100mm Culture Tubes 13X100 mm Screw Caps 20X150mm Culture Tubes

Pipets 5 3/4" Pipets 9" Pipets

Attachment II: Government Furnished Property

Glassware Inspection Area 2 Dry Sterilizers 2 Pipette Plugger 6 Delivery Carts 9 Metal Carts for Sterile Glassware 6 Glassware Bucks

Wash Room 2 Tunnel Washers 1 Steam Kettle for Fly Media 1 Dispenser 1 Manual Glass Washer 1 Washer & Dryer 1 Freezer (receiving area) 2 Autoclave Sterilizers (hallway) 2 Refrigerators 1 Old cart washer

Pour Room 2 Hoods 3 Plate Dispensers 2 Water Baths

General 1 Hazardous Chemical Cabinet 1 Exhaust Hood 3 Desktop Computers with Monitors 2 Laser Printer 2 Smart Label Printer 3 Dl Water System (pump, container, timer) 2 PicoPure Filter System 2 PH Meters 1 Stove Top 2 Pumps for dispensing

28 Stir Plates 5 Balances 1 Autoclave (D-103) 1 Walk-in Cold Room (D103) 1 Incubator (D103) 3 Autoclave Carts

Assortment of glassware and plastic ware

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 4700 813 968 4 74 720 November 12600 1166 1378 1400 239 440 December 13800 2712 1400 205 248 360 January 12500 1593 1773 200 134 9 February 12600 2080 1493 200 195 760 March 19000 1160 2099 130 252 1080 April 17200 320 1761 180 249 940 May 12300 1130 2232 354 183 1160 June 15700 1160 1814 200 352 1300 July 15700 609 2232 200 296 1160 August 15800 1160 2491 1200 249 320 September 13300 928 2406 140 253 928 Total 165,200 14,831 22,047 4,413 2,724 9,177

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 3270 214 586 100 40 720 November 11648 239 379 100 125 320 December 6312 125 216 80 145 January 8900 110 308 80 132 680 February 8716 616 162 110 89 2104

March 9040 508 625 180 180 1342 April 7231 512 464 120 143 360 May 6702 414 1395 100 116 1342 June 10656 363 556 120 163 1088 July 10879 464 666 100 152 1632

August 6680 1058 323 140 244 200 September 10557 508 467 120 115 792

Total 100,591 5,131 6,147 1,350 1,644 10,580

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 6382 162 509 80 4 720 November 4317 262 531 120 125 440 December 6498 720 1220 180 145 360 January 13449 343 855 80 132 3736 February 8008 256 573 80 89 1000

March 5412 465 765 180 180 1704 April 7814 180 356 120 143 720 May 4845 622 922 100 116 2616 June 9530 496 816 120 163 1960 July 9198 609 1153 100 152 1342

August 13300 928 1203 80 250 1984 September 15800 1160 1731 20 252 1203

Total 104,553 6,203 10,634 1,260 1,751 17,785

FY 2013

FY 2014

FY 2015

Attachment IV: Media Production, FY 2013-FY 2015

Attachment V: Standard Order Media Products

# 0 Minimal Medium Plates

#0 / MINIMAL MEDIUM PLATES w/kan.25ug/ml

#07/ MINIMAL MEDIUM PLATES W/LACTOSE

#21 MINIMAL MEDIUM WITH IPTG (red/brown)

#4 LB PLATES (LURIA AGAR PLATES) <BTL>

#4 LB PLATES (LURIA AGAR PLATES) <DISH>

#45 LB + STRIPTOMYCIN cc: yellow-green

#5 P-GAL MEDIUM (color: green)

#54 LB + RIF color code: green-yellow

#60 MINIMAL MEDIA CC: BLUE,BLACK

#65 MM - PLATES cc: blue_green

#66 MM - PLATES (color code:blue-blue)

#69 Minimal Agar

(MEM) ALPHA MEDIUM

1.5% AGAR

10% EDTA DECALIFATION of BONE

10% NEUTRAL BUFFERED FORMALIN

10X PBS Ca-Mg Free

10X TBE

10X TRIS BUFFERED SALINE

13x100 tubes

1M MgCL2 SOLUTION

20 X SSC

2X YT MEDIA

50X TAE BUFFER

50X VB Medium Conc.

https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

5X TBE

ACK LYSIS BUFFER (GROVES)

ALG #2 (LEU DROP W/2% GALACTOSE

alternative recipe for versene

BAP (TRYPTOCASE SOY AGAR)

BG PLATES

BHI = BOVINE HEART INFUSION AGAR PLATES

brain heart infusion medium

BREWER'S THIOGLYCOLLATE BROYH

BRUCILLA AGAR W/TVP (CULTURE TUBE)

BRUCILLA AGAR W/TVP (PETRI DISHES)

Can Agar w/o URA

CAN PLATES W/O LEU

CAN PLATES W/O TRP.

CAN,GAL-COMPLETE PLATES

CDO -URA -TRP SCGL Recipe

CDO -TRP SCGL Recipe

CDO YPGL Recipe

CHA1 PLATES

CHA2 PLATES w/o ADENINE

CHOCOLATE AGAR PLATES

Chocolate Agar with IsoVitalex Plate

Columbia Blood Agar

COM AGAR W/O TRP,URA, & LEU

COM AGAR W/O URA and TRP W/ SORB (UTTOP)

COM PLATES W/O HIS and LEU.

COM PLATES W/O LYS and LEU.

COM PLATES W/O URA and LEU.

COM PLATES W/O TRP-LEU

COM PLATES W/O TRP-LEU-HIS

Com w/o trp plates

Com-Trp- & Ura Plates

COMP MIN DROPOUT PLATE W/O URACIL (btl)

COMP MIN DROPOUT PLATE W/O URACIL (dish)

DMEM (high glucose) GIBCO # 11965118

DMEM LOW GLUCOSE GIBCO #11885076

ENT PLATES (M-ENTEROCOCCUS AGAR)

G418-YPDA

GAL - URA PLATES

GAL- TRYP.PLATES

GAL-LYSINE PLATES

GAL-PE-LEU.PLATES

GLUTAMAX-1 SUPPLEMENT

GRAM NEGATIVE BROTH (GN)

H 40 PLATES

H-40 X PLATES

HAM'S F-10 nutrient mix. gibco # 81200

HAM'S F-12 GIBCO CAT 11765062

Hank's Balance Salt Solution w/ Ca & Mg

HANK'S BALANCED SALT SOLUTION-CMF

HEPES BALANCD SALT SOLUTION W/PENN-STREP

HEPES BUFFER

HG AGAR = HIGH GROWTH PLATES

Hyg-YPDA

ISCOVE'S MODIFIED DULBECCO'S (IMDM)

Isotonic Hepes

K-AGAR PLATES

K-agar plates (100 mm)

K-AGAR PLATES (60mm)

L-AGAR PLATES and BROTH LOW SODIUM (dsh) https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

L-AGAR PLATES W/KANAMYCIN

LB (LURIA-BERTANI) MEDIUM)

LB + KANAMYCIN (cc: #17 brown-purple)

LB + TETRACYCLINE (15 ug/ml)

LB + TETRACYCLINE (15 ug/ml) + AMP

LB AGAR W/AMP PLATES

LB Agar w/Chloramphenical,25ug/ml

LB AGAR WITH AMPICILLIN (50 UG/ML) DISH

LB AGAR WITH AMPICILLIN + TETRACYCLINE

LB AGAR/TETRACYCLINE W/15 ug/ml

LB SOFT AGAR

LB W/AMP PLATES COLOR CODE - #47

LB W/TETRACYCLINE 15 ug/ml Medium #15

LB WITH KANAMYCIN AND X-GAL

LB/AMP/X-GAL/IPTG* PLATES

LB/KAN/X-GAL + IPTG

LB/TET/AMP/X-GAL/IPTG* PLATES

LCTG MEDIA PLATES CODE #63

LITMUS MILK

Low salt LB Agar with Zeocin 25mg/liter

LYSINE IRON AGAR (LIA) SLANTS DEEP

M-9 Plates w/Thiamine

M9 Medium

MAC PLATES

Malt Extract Agar

MANNITOL SALT AGAR

MAR # 11 MET AGAR PLATES

MAR # 12 PHE AGAR PLATES

MAR # 13 SER AGAR PLATES (brown-orange)

MAR # 14 THR AGAR PLATES (brown/yellow) https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

MAR # 16 TYR AGAR PLATES color-cd:yellow

MAR # 18 VAL AGAR PLATES

MAR # 20 CAN AGAR PLATES

MAR # 4 ARG AGAR PLATES

MAR # 5- ASP AGAR PLATES

MAR # 6 GLU AGAR PLATES color-code:BLUE

MAR # 9. LEU AGAR PLATES

MAR #10 LYS AGAR PLATES

MAR #15 TRP AGAR PLATES (brown/green)

MAR #17: URA AGAR PLATES (brown/purple)

MAR #1:MIN AGAR PL and BROTH (PTR DISH)

MAR #22 CAN-FOA AGAR PLATES

MAR #22 CAN-FOA AGAR Plates

MAR #23:CAN-low_ADE AGAR PLATES

MAR #24:C0M-low_ADE AGAR PLATES

MAR #2: C0M AGAR PLATES Color/Code: red

MAR #2: COM AGAR PLATES

MAR #3: ADE AGAR PLATES

MAR #7: HIS AGAR PLATES colr code:purple

McCOY'S 5A MEDIUM W/O PENN and STREP

MEM (GIBCO # 10370021

MEM Medium gibco# 11400(equiv.to#11385

MEM w/ EARLES SALTS w/o L-GLUTAMINE

MEM W/EARLE'S SALTS W/O GLUTAMINE

MEM w/o glutamine (Sigma# M3024) minimal non inducing media

MR/VP BROTH

MSA = MANNITOL SALT AGAR PLATES

MYCOSEL AGAR

NEMATODE AXENIC MEDIUM

NGH BROTH = NG W/O AGAR

NGM AGAR= NEMATODE GROWTH MEDIUM AGAR

NZY BROTH

Oocyte Ringer 2 (OR2)

OPTI-MEM GIBCO CAT#31985-070

PBS - CMF

PBS CMF (Gibco / sheri)

PBS with Ca & Mg

PBS-CMF w/o KCL

PBS-CMF W/SODIUM AZIDE

PHENOL RED BROTH W/1% ADONITAL

POTATO DEXTROSE AGAR (TUBE)

POTATO DEXTROSE W/P-S CYCLOHEXAMIDE

Powdered COM-sugar-hallut

PS Gal w/o Leu & Lys

PS Gal W/O TRP & LEU

PS Gal w/o Trp,Leu, & Lys.

PS Gal w/o Trp,Leu,& His

PSEUDOMONAS/POUR PLATES

PSEUDOMONAS/POUR TUBES

Puck's Saline "A"

RPMI 1640 Rice

RPMI GIBCO #21870 W/O L-GLUTAMINE

RPMI-1640 (GIBCO CAT # 11875119

RPMI-1640 w/o Phenol Red ,Cat# 13200

RYM #36: BC PLATES

RYM #37: BC-TRP PLATES

RYM 13: SP3-22 AGAR PLATES

RYM 14: MIN AGAR PLATES W/ URA and LEU

RYM 15/C: GAL-C0M AGAR PLATES

RYM 1: YPD BROTH

RYM 26: LEU AGAR PLATES

RYM 26: LEU AGAR PLATES W/SORBITOL

RYM 26: LEU AGAR W/SORBITOL (LTOP)

RYM 31: TRP BROTH

RYM 3: YPDA AGAR PLATES

RYM 5: YPG AGAR PLATES

S S AGAR PLATES

S.O.C. MEDIA

S.O.C. MEDIUM

SABOURAUD DEXTROSE AGAR (PETRI DISH)

SABOURAUD DEXTROSE AGAR (TUBES)

SABOURAUD DEXTROSE AGAR SLANTS

SCGL Broth

SD SER #1

SD-URA PLATES

SDS PAGE BUFFER (10X)

Selinite

SOB BROTH

SOFT AGAR (13 X 100 TUBES)

SOFT AGAR (KIMBLE BOTTLES)

STARCH AGAR

Sterile Culture tube 13x100 mm sterile culture tubes

Sterile Toothpicks

TB1 Agar, bottle (Wilson)

TB1 Agar, plates (Wilson)

TB1 PLATES and TOP AGAROSE (PETRI DISH) https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

TB1 Top Agar, bottle (Wilson)

TERRIFIC BROTH

TERRIFIC BROTH w/o glycerol

TETRAT BROTH W/0.001% BRILLIANT GREEN

Todd Hewitt Broth

TRANSFER BUFFER (tris glycine)

TRANSPORT MEDIUM

Triton Lysis Buffer

TRP AGAR PLATES EX- AD and HIS

TRYPTONE GLUCOSE EXTRACT AGAR

TSA = TRYPTIC SOY AGAR PLATES

TSI SLANTS

VERSENE

VIOLET RED AGAR

Whitten's + HEPES Medium

Whitten's Midium

Williams

Williams 'E' Medium w/P &S

WILLIAMS' MEDIUM 'E' HAM'S F-12 1:1 MIX

YAS-1: YPDA BROTH

YES (Yeast Extract with Supplements)

YNB

YP-Gal Broth

YP-GAL PLATES

YPD + Zeocin

YPD AGAR PLATES

YPD BROTH

YT AGAR PLATES

YT MEDIA

ZP 5FOA Plates

ZP 5FOA(R/G) Plates

ZP SC(R/G)-URA

ZP YP(R/G)A BROTH

ZP YP(R/G)A PLATES

https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist https://apps.niehs.nih.gov/moats/index.cfm?action=home.picklist

Attachment I: Government Owned Glassware and Pastic Items

Glass Items Plasic Items

Beakers Flasks 30ml Tissue Culture Flask 50ml 100ml Filters 150ml 115ml 250ml 150ml 400ml 500ml 600ml 1000ml 1000ml Tissue Culture Filters 2000ml 4000ml Trays

Animal Trays Cylinders 10ml Petri Dishes 25ml 60X15mm 50ml 100X15mm 100ml 150X25mm 250ml 500ml Caps for Tubes 1000ml 13X100 Caps 2000ml 20X150 Caps

Cylinders Carboys w/stoppers 10ml 25ml 50ml 100ml 250ml 500ml 1000ml 2000ml

Bellco Flasks 25ml 60ml 125ml 250ml

Erlenmeyer Flasks 25ml 50ml 125ml 250ml 500ml 1000ml 2000ml 4000ml

Filtering Flasks 25ml 50ml 125ml 250ml 500ml 1000ml 2000ml 4000ml

Volumetric Flasks 10ml 25ml 50ml 100ml 200ml 250ml 500ml 1000ml 2000ml 4000ml

Erlenmeyer Flasks w/stoppers 25ml 50ml 125ml 250ml 500ml 1000ml

Kimble Bottle 100ml

Kimble Bottles w/small mouth 100ml 500ml 1000ml

Specimen Bottles 16ml 6ml

Reagent Bottles 500ml 1000ml 2000ml 4000ml

Solution Bottles Wide Mouth Big Wide Mouth Small Small Mouth

Jugs

Fern Back Flask

Jars 16 oz 16oz wide mouth

Tubes 6X50mm Durham Tubes 13X100mm Culture Tubes 13X100 mm Screw Caps 20X150mm Culture Tubes

Pipets 5 3/4" Pipets 9" Pipets

Attachment II: Government Furnished Property

Glassware Inspection Area 2 Dry Sterilizers 2 Pipette Plugger 6 Delivery Carts 9 Metal Carts for Sterile Glassware 6 Glassware Bucks

Pour Room 2 Hoods 3 Plate Dispensers 2 Water Baths

28 Stir Plates 5 Balances 1 Autoclave (D-103) 1 Walk-in Cold Room (D103) 1 Incubator (D103) 3 Autoclave Carts

Assortment of glassware and plastic ware

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 3270 214 586 100 40 720 November 11648 239 379 100 125 320 December 6312 125 216 80 145 January 8900 110 308 80 132 680 February 8716 616 162 110 89 2104

March 9040 508 625 180 180 1342 April 7231 512 464 120 143 360 May 6702 414 1395 100 116 1342 June 10656 363 556 120 163 1088 July 10879 464 666 100 152 1632

August 6680 1058 323 140 244 200 September 10557 508 467 120 115 792

Total 100,591 5,131 6,147 1,350 1,644 10,580

Month Plates (L) Buffer/Tissue Culture (L) Broth (L) PBS Carboy

(L) Premade (L) Culture tube (L)

October 6382 162 509 80 4 720 November 4317 262 531 120 125 440 December 6498 720 1220 180 145 360 January 13449 343 855 80 132 3736 February 8008 256 573 80 89 1000

March 5412 465 765 180 180 1704 April 7814 180 356 120 143 720 May 4845 622 922 100 116 2616 June 9530 496 816 120 163 1960 July 9198 609 1153 100 152 1342

August 13300 928 1203 80 250 1984 September 15800 1160 1731 20 252 1203

Total 104,553 6,203 10,634 1,260 1,751 17,785

FY 2013

FY 2014

FY 2015

Attachment IV: Media Production, FY 2013-FY 2015

Attachment V: Standard Order Media Products

# 0 Minimal Medium Plates

#0 / MINIMAL MEDIUM PLATES w/kan.25ug/ml

#07/ MINIMAL MEDIUM PLATES W/LACTOSE

#21 MINIMAL MEDIUM WITH IPTG (red/brown)

#4 LB PLATES (LURIA AGAR PLATES) <BTL>

#4 LB PLATES (LURIA AGAR PLATES) <DISH>

#45 LB + STRIPTOMYCIN cc: yellow-green

#5 P-GAL MEDIUM (color: green)

#54 LB + RIF color code: green-yellow

#60 MINIMAL MEDIA CC: BLUE,BLACK

#65 MM - PLATES cc: blue_green

#66 MM - PLATES (color code:blue-blue)

#69 Minimal Agar

(MEM) ALPHA MEDIUM

1.5% AGAR

10% EDTA DECALIFATION of BONE

10% NEUTRAL BUFFERED FORMALIN

10X PBS Ca-Mg Free

10X TBE

10X TRIS BUFFERED SALINE

13x100 tubes

1M MgCL2 SOLUTION

20 X SSC

2X YT MEDIA

50X TAE BUFFER

50X VB Medium Conc.

5X TBE

ACK LYSIS BUFFER (GROVES)

ALG #2 (LEU DROP W/2% GALACTOSE

alternative recipe for versene

BAP (TRYPTOCASE SOY AGAR)

BG PLATES

BHI = BOVINE HEART INFUSION AGAR PLATES

brain heart infusion medium

BREWER'S THIOGLYCOLLATE BROYH

BRUCILLA AGAR W/TVP (CULTURE TUBE)

BRUCILLA AGAR W/TVP (PETRI DISHES)

Can Agar w/o URA

CAN PLATES W/O LEU

CAN PLATES W/O TRP.

CAN,GAL-COMPLETE PLATES

CDO -URA -TRP SCGL Recipe

CDO -TRP SCGL Recipe

CDO YPGL Recipe

CHA1 PLATES

CHA2 PLATES w/o ADENINE

CHOCOLATE AGAR PLATES

Chocolate Agar with IsoVitalex Plate

Columbia Blood Agar

COM AGAR W/O TRP,URA, & LEU

COM AGAR W/O URA and TRP W/ SORB (UTTOP)

COM PLATES W/O HIS and LEU.

COM PLATES W/O LYS and LEU.

COM PLATES W/O URA and LEU.

COM PLATES W/O TRP-LEU

COM PLATES W/O TRP-LEU-HIS

Com w/o trp plates

Com-Trp- & Ura Plates

COMP MIN DROPOUT PLATE W/O URACIL (btl)

COMP MIN DROPOUT PLATE W/O URACIL (dish)

DMEM (high glucose) GIBCO # 11965118

DMEM LOW GLUCOSE GIBCO #11885076

ENT PLATES (M-ENTEROCOCCUS AGAR)

G418-YPDA

GAL - URA PLATES

GAL- TRYP.PLATES

GAL-LYSINE PLATES

GAL-PE-LEU.PLATES

GLUTAMAX-1 SUPPLEMENT

GRAM NEGATIVE BROTH (GN)

H 40 PLATES

H-40 X PLATES

HAM'S F-10 nutrient mix. gibco # 81200

HAM'S F-12 GIBCO CAT 11765062

Hank's Balance Salt Solution w/ Ca & Mg

HANK'S BALANCED SALT SOLUTION-CMF

HEPES BALANCD SALT SOLUTION W/PENN-STREP

HEPES BUFFER

HG AGAR = HIGH GROWTH PLATES

Hyg-YPDA

ISCOVE'S MODIFIED DULBECCO'S (IMDM)

Isotonic Hepes

K-AGAR PLATES

K-agar plates (100 mm)

K-AGAR PLATES (60mm)

L-AGAR PLATES W/KANAMYCIN

LB (LURIA-BERTANI) MEDIUM)

LB + KANAMYCIN (cc: #17 brown-purple)

LB + TETRACYCLINE (15 ug/ml)

LB + TETRACYCLINE (15 ug/ml) + AMP

LB AGAR W/AMP PLATES

LB Agar w/Chloramphenical,25ug/ml

LB AGAR WITH AMPICILLIN (50 UG/ML) DISH

LB AGAR WITH AMPICILLIN + TETRACYCLINE

LB AGAR/TETRACYCLINE W/15 ug/ml

LB SOFT AGAR

LB W/AMP PLATES COLOR CODE - #47

LB W/TETRACYCLINE 15 ug/ml Medium #15

LB WITH KANAMYCIN AND X-GAL

LB/AMP/X-GAL/IPTG* PLATES

LB/KAN/X-GAL + IPTG

LB/TET/AMP/X-GAL/IPTG* PLATES

LCTG MEDIA PLATES CODE #63

LITMUS MILK

Low salt LB Agar with Zeocin 25mg/liter

LYSINE IRON AGAR (LIA) SLANTS DEEP

M-9 Plates w/Thiamine

M9 Medium

MAC PLATES

Malt Extract Agar

MANNITOL SALT AGAR

MAR # 11 MET AGAR PLATES

MAR # 12 PHE AGAR PLATES

MAR # 13 SER AGAR PLATES (brown-orange)

MAR # 16 TYR AGAR PLATES color-cd:yellow

MAR # 18 VAL AGAR PLATES

MAR # 20 CAN AGAR PLATES

MAR # 4 ARG AGAR PLATES

MAR # 5- ASP AGAR PLATES

MAR # 6 GLU AGAR PLATES color-code:BLUE

MAR # 9. LEU AGAR PLATES

MAR #10 LYS AGAR PLATES

MAR #15 TRP AGAR PLATES (brown/green)

MAR #17: URA AGAR PLATES (brown/purple)

MAR #1:MIN AGAR PL and BROTH (PTR DISH)

MAR #22 CAN-FOA AGAR PLATES

MAR #22 CAN-FOA AGAR Plates

MAR #23:CAN-low_ADE AGAR PLATES

MAR #24:C0M-low_ADE AGAR PLATES

MAR #2: C0M AGAR PLATES Color/Code: red

MAR #2: COM AGAR PLATES

MAR #3: ADE AGAR PLATES

MAR #7: HIS AGAR PLATES colr code:purple

McCOY'S 5A MEDIUM W/O PENN and STREP

MEM (GIBCO # 10370021

MEM Medium gibco# 11400(equiv.to#11385

MEM w/ EARLES SALTS w/o L-GLUTAMINE

MEM W/EARLE'S SALTS W/O GLUTAMINE

MEM w/o glutamine (Sigma# M3024) minimal non inducing media

MR/VP BROTH

MSA = MANNITOL SALT AGAR PLATES

MYCOSEL AGAR

NEMATODE AXENIC MEDIUM

This is the start of the file's text. The full file is on GovTribe.

File details come from the government source that posted it. Updated Nov 14, 2017.