PD.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Brand name or Equal Automated and Unbiased Stereological and Quantitative Neuron Matrix Analysis System for the Synaptic Function Section

1. Statement of Need and Purpose: The purpose of this acquisition is to acquire a Neurolucida and Stereo Investigator System or equal system for an existing Nikon Ti-E microscope to provide Investigators the ability to implement design based stereology protocols and morphometric quantitative analysis of neurons.

2. Background Information and Objective: The Synaptic Function Section research focuses on the molecular mechanisms underlying neurotransmitter release and its modulation. In particular, SFS investigators are researching neurodegenerative diseases, neurological diseases and adult neurogenesis. A custom, fully integrated, Neurolucida and Stereo Investigator system or Equal is required to upgrade an existing Nikon Ti-E Microscope to advance analysis of neurons and neurites.

3. Generic Name of System: Automated and unbiased stereological and quantitative neuron matrix analysis system.

4. Purchase Description:

Trade name: N/A System’s brand name: Neurolucida and Stereo Investigator System Manufacturer’s name: MicroBrightField, Inc. (MBF Bioscience) Model, Type, catalog and/or part numbers:

Item Number Item Name Descripiton SS-15 Stereo Investigator

Software Designed based sterology automated workflow software that allows for 3D image stacking acquisition, contour tracing, serial sectioning, 2 D anatomical mapping. Exports data in excel.

NL-15 Neurolucida Software Software that allows for 3D neuron tracing and reconstruction, 3D serial reconstruction, brain and anatomical mapping, quanatitive morphometric and image analaysis, real time voxel rendering, 3D visualization traced models, and cell detection.

Exports data in excel.

NS-21 Image Stack Extension Module

Module that allows for 3D image visualization as well as analyze confocal bright fields and wide field fluorescence 3D image stacks.

NS-22 2D Slide Scanning Module

Module upgrade that provides the ability to combine high resolution images from multiple microscopes.

NS-25a Upgrade 2D Slide to 3D Slide Scanning Module

Module that provides the ability to create 3D virtual tissue images.

MM-10 Image Montaging Software that allows for alignment of 2D and 3D

Software Extension Module images in XY and Z axis.

MS-S5 Software Integration Module

Software that provides the ability to automatically control microscope functions.

CI-21 Software for Coolsnap

ES2

Photometrics camera Software

FL-PRO220-DRV Software Driver Software driver for Lumen220Pro MS-S2 Software Integration Integration software for driver shutter and filter wheels MS-64a 2 Axis computer control motor system CPU control motoring system

MS-10 Focus Measurement Encoder

Encoder that provides the ability to focus the actal microscope stage position.

DV-47d Color Camera Camera that allows for 1600Hx1200V at 10 frames per second and includes firewire PC interface card.

CM-10 1.0x DC Series C-mount adpater

Camera mount adapter

PC-90a-SSD Workstation 64 bit Windows workstation ML-4k-27 Monitor 2 - 27 in, 4K monitors PC-42a Uninterruptable Power

Supply(UPS) 155VA UPS battery with surger protector

CS-10 Calibration Grid Slide Calibration Grid Slide TK-10 MBF ToolKit English and metric ball hex wrenches, key set and screwdriver specific to software and hardware installtion

MQS 41100 Motorized Stage Control Motorized stage control device MQS31410 NIS upgrade NIS elements software upgrade MEL59500 DIC Condensor Lens

Extension Mount Extension mount for lens

MEL51220 0.85 Condensor Lens Dry condenser lens

5. Salient characteristics: The Contractor shall provide the SFS with the following Neurolucida and Stereo Investigator system [MBF Item Numbers: SS-15, NL-15, NS-21, NS-22, NS-25a, MM-10, MS-S5, CI-21, FL-PRO220-DRV, MS-S2, MS-64a, MS-10, DV-47d, CM-10, PC-90a- SSD, ML-4k-27, , PC-42a, CS-10, TK-10, MQS 41100, MQS31410, MEL59500, MEL51220] or Equal that meets the following specifications:

• System shall have the capability for 3D tracing of neuronal structures from a live camera image across multiple fields of view and multiple sections to create a complete 3D reconstruction including all XYZ position data that can then be analyzed to generate quantitative data.

• System shall be able to provide a full spectrum of unbiased design based stereological protocols and analyses using scientifically accepted practices for efficient estimation of cell populations within a specified region of interest, and to measure lengths, areas and volumes within a biological region of interest.

• System shall include a linear encoded XY motorized stage, Z axis linear encoder and high resolution color camera for imaging with drivers.

• System shall include software that is compatible with and can fully integrate Nikon Ti-E microscope components such as a Prior ProLumen 220 light source, shutter and filter wheel system, Coolsnap ES2 camera, linear coded XY motorized stage and high resolution camera.

• The system shall have the ability to be fully integrated with an existing Nikon Ti-E microscope to control motorized components for automated analysis that are used for neurodegeneration and adult neurogenesis research.

• System shall include software for acquiring 3D image stacks, 2D whole slide scanning and 3D whole slide scanning, and automated image montaging of image stacks acquired on other imaging platforms.

• System shall include software that updates existing NIS Elements software in order to control a linear encoded XY motorized stage and high resolution color camera.

• System software packages shall be compatible with and have the ability to read each other native data files as well as existing Neurolucida native data files.

• System software shall include drivers used for controlling all Nikon Ti-E microscope hardware.

• System shall include a PC workstation, at least 2 monitors and uninterruptable power supply (UPS).

• The Contractor shall provide a minimum of a one (1) year warranty. All products shall be warranted to be free from material defects in workmanship or material under normal use and service for warranty period.

6. Quantity:

1. Neurolucida and Stereo Investigator or Equal System. 1 each

2. Shipping & Handling 1 each

2. Software upgrades and remote online and telephone technical support by an OEM certified technican 1 year (date of installation for 12 months)

7. Delivery Date:

a. Contractor shall deliver one (1) Neurolucida and Stereo Investigator or Equal System within 90 days of receipt of order.

b. Contactor shall provide installation and three (3) day on-site training within 30 days of delivery. System synchronization with existing Nikon Ti-E microscope shall occur at time of installation. Delivery, installation, synchronizationand training shall take place between the hours of 8:00 am and 5:00 pm Bethesda, MD local prevailing time, Monday through Friday at 35 Convent Drive, Room

2B706, Bethesda, MD 20892. Installation, synchronization and training shall be provided by an original equipment manufacturer (OEM) certified service technician.

9. Option(s): Contractor shall provide the Government an option for annual software upgrades and remote online and telephone technical support by an OEM certified technican for two years.

ii. Option Period One: TBD (effective day after expiration of year one)

iii. Option Period Two: TBD (effective day after expiration of option period One)