Project Grant R43OD032088

Award Date 9/15/22
Completion Date 9/14/24
Dollars Obligated $518K
Funding Federal Agency
Office of the Director
Federal Grant Program
93.351
Assistance Type
Project Grant
Place of Performance
Lexington, KY 40505, USA
Similar Awards
The Project Grant award R43GM156169 for $306,852.00, provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), aims to develop and validate cryopreservation technology that automates the soaking, cooling, and warming of small biological samples such as oocytes and embryos. The key objectives are to optimize convective heat transfer to achieve ultra-fast cooling and warming rates, eliminate ice formation and...
The National Institute of Child Health and Human Development (NICHD) awarded a $275,001 Project Grant under the Child Health and Human Development Extramural Research (CFDA 93.865) program to Jun Innovations Inc. to develop a novel supercooling technology for preserving biological materials like ovaries at subzero temperatures without freezing. The goal is to enable extended storage durations and higher recovery rates of ovarian functionality compared to traditional cryopreservation methods,...
This Project Grant award of $185,500 from the National Institutes of Health (NIH) Office of Research Infrastructure Programs (CFDA 93.351) will enable Idaho State University (ISU) to detect, monitor, and track extrinsic factors such as temperature, humidity, light, noise, and vibration within the animal research facility. The goal is to improve research outcomes, animal welfare, and facility operations by maintaining consistent and ideal environmental conditions for research animals, reducing...
This $550,000 Project Grant from the US Department of Agriculture's National Institute of Food and Agriculture (NIFA) under the Agriculture and Food Research Initiative (AFRI) program will fund research at Iowa State University to improve understanding and assessment of boar sperm fertility. The university will identify biochemical differences between more and less fertile boar sperm to better understand the molecular mechanisms of fertility and infertility. Researchers will also translate sperm...
This Project Grant award from the National Institute of Child Health and Human Development (CFDA 93.865 - Child Health and Human Development Extramural Research) provides $192,056 to the Magee-Womens Research Institute and Foundation, a non-profit organization located in Pittsburgh, Pennsylvania, to research the feasibility and safety of using adeno-associated virus (AAV) gene therapy to restore fertility in mammalian models of non-obstructive azoospermia. The key research objectives are to 1)...
The U.S. National Institutes of Health (NIH) awarded a $349,584 Project Grant under the Research Infrastructure Programs (CFDA 93.351) to Rutgers, The State University of New Jersey, to outfit 14 individually ventilated animal caging racks with a digital ventilated cage (DVC) retrofit kit and rack environmental monitoring (REM) system. This advanced caging system will utilize machine learning and artificial intelligence to monitor the animal facility 24/7, enabling improved animal welfare,...
This Project Grant award from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) under the Diabetes, Digestive, and Kidney Diseases Extramural Research program (CFDA 93.847) provides $305,093 to Carnegie Mellon University (CMU) to develop innovative pressure-modulated cryopreservation systems for high-subzero liver preservation. The project aims to advance organ preservation methods beyond traditional isochoric cooling to eliminate the risk of ice formation and...
This $349,788 Project Grant awarded by the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351) will establish a state-of-the-art vivarium dedicated to the UTRGV laboratory opossum (Monodelphis domestica) research resource. The funds will be used to purchase and install 10 racks of an individualized ventilated cage (IVC) system, providing 1,300 cages for individually housed opossums and 546 cages for mated pairs, mothers with litters, and groups of...
This Project Grant awarded by the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351) provides $294,543 to Objective Biotechnology, Inc. to develop a machine vision guided robotic system for automating microinjections into fruit fly (Drosophila melanogaster) embryos. The goal is to eliminate the need for manual, labor-intensive microinjection protocols and accelerate the process of generating and maintaining transgenic fly lines, which are critical for...
The Department of Agriculture's National Institute of Food and Agriculture awarded $650,000 under the Agriculture and Food Research Initiative competitive grants program to the University of Nebraska from May 2023 through April 2026. The project aims to address heat stress impacts on swine production through three objectives: determining effects on boar reproductive endocrinology, assessing impacts to sperm production, health and fertility; and investigating impairments to cellular and molecular...

A NOVEL RAT SPERM CRYOPRESERVATION AND ARTIFICIAL INSEMINATION SYSTEM - SUMMARY PARATECHS CORPORATION AIMS TO PROVIDE RESEARCHERS WITH ADVANCED TECHNOLOGY AND METHODS TO SIMPLIFY PROCEDURES FOR LABORATORY ANIMAL MODELS. THIS PROPOSAL IS A DIRECT RESPONSE TO PAR-21-225; NOVEL TOOLS AND DEVICES FOR ANIMAL RESEARCH FACILITIES AND TO SUPPORT THE CARE OF ANIMAL MODELS. THE GOAL IS TO IMPROVE VIVARIUM MANAGEMENT THROUGH CRYOPRESERVATION, STRATEGIC BREEDING, AND ORGANIZATIONAL APPROACHES CURRENTLY UNAVAILABLE FOR RATS. THE RAT ARTIFICIAL INSEMINATION SYSTEM (RAIS) IS A COMPLETE RAT SPERM CRYORECOVERY SYSTEM. THIS SYSTEM WILL REVOLUTIONIZE CRYOPRESERVATION PRACTICES AND VIVARIUM MANAGEMENT FOR RAT MODELS. FIRST, SPERM IS CRYOPRESERVED IN A "CRYODROPPER" DEVICE, WHICH WILL QUICKLY AND EASILY FREEZE RAT SPERM. SECOND, SPERM IS RECOVERED IN A NOVEL MOTILITY ENHANCING DEVICE (MED) WHICH SELECTS FOR MOTILE SPERM, REMOVES CRYOPROTECTANTS, AND CONCENTRATES THE SPERM SAMPLE. THE THIRD PART OF THE SYSTEM IS A NOVEL DEVICE, WHICH IS USED TO TRANSFER CONCENTRATED MOTILE SPERM TO THE UTERINE HORN OF RECIPIENT FEMALE RATS. THIS NON-SURGICAL INSEMINATION DEVICE (NSID) PROVIDES A NON-SURGICAL ALTERNATIVE TO TRADITIONAL SURGICAL ARTIFICIAL INSEMINATION AND REQUIRES NO ANESTHESIA OR ANALGESIA. EACH INNOVATIVE PART OF THE SYSTEM PROVIDES A CRITICAL FUNCTION FOR RAT CRYORECOVERY AND TOGETHER PROVIDE A COHESIVE SYSTEM FOR RAPID, INEXPENSIVE, AND EFFICIENT VIVARIUM MANAGEMENT. FEASIBILITY OF THE RAIS WILL BE PROVEN IN THE FOLLOWING AIMS; 1) OPTIMIZE THE CRYODROPPER FOR RAT SPERM CRYOPRESERVATION, 2) MANUFACTURE AND TEST THE MED FOR MOTILE SPERM ENRICHMENT AND RECOVERY, AND 3) MANUFACTURE AND TEST THE RAT NSID DEVICE WITH FRESH AND CRYOPRESERVED SPERM. THE SYSTEM WILL PROVIDE SUBSTANTIAL COST SAVINGS VERSUS CONTRACTED CRYOPRESERVATION SERVICES AND INCREASE THE SIMPLICITY AND RELIABILITY OF RAT SPERM CRYOPRESERVATION PROCEDURES. RAIS WILL DIRECTLY BENEFIT THE WELFARE OF LABORATORY ANIMALS BY REDUCING THE NUMBER OF ANIMALS KEPT IN IDLE COLONIES, IMPROVING VIVARIUM MANAGEMENT OPTIONS, AND PROTECTING RAT MODELS FROM CATASTROPHIC LOSS DUE TO NATURAL DISASTERS, GENETIC DRIFT, DISEASE, AND BREEDING PROBLEMS. RAIS IS DESIGNED TO PROVIDE BOTH A REDUCTION IN ANIMAL NUMBERS AND A REFINEMENT TO MINIMIZE ANIMAL SUFFERING AND IMPROVE WELFARE; MEETING THE REQUIREMENTS OF THE 3RS OF ANIMAL RESEARCH.

Posted 9/6/22, 12:00 AM