Project Grant R35GM153191
- The National Science Foundation Division of Chemistry awarded a $1,010,000 project grant to the University of Texas at Austin to develop innovative mass spectrometry methods for characterizing intact proteins through June 2025 under the Mathematical and Physical Sciences program (CFDA 47.049). Professor Jenny Brodbelt and her research group will apply ultraviolet photodissociation and other dynamic ion manipulation strategies to amplify structural and post-translational modification details...
- The National Science Foundation's Integrative Activities (CFDA 47.083) program has awarded a $350,000 Project Grant to the College of Charleston to acquire a MALDI-TOF/TOF mass spectrometer instrument. This state-of-the-art equipment will enable new research directions, encourage cross-disciplinary collaborations, and provide hands-on training for a diverse population of undergraduate students and researchers at the college and across the Lowcountry region of South Carolina. The mass...
- This Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), aims to advance the field of native mass spectrometry (MS) analysis. The $316,825 award to Massmatrix Inc., effective May 1, 2025 through April 30, 2026, will fund the development of an innovative intact mass deconvolution method to significantly improve the speed, accuracy, and user-friendliness of protein and nucleic acid molecular...
- The National Science Foundation (NSF) Division of Chemistry awarded a $348,737 Project Grant to Mississippi State University to acquire a high-resolution quadrupole time-of-flight mass spectrometer with diverse inlet and ionization capabilities for chemical analyses. This award is jointly funded through the Major Research Instrumentation Program, Chemistry Research Instrumentation Program, and Established Program to Stimulate Competitive Research. The new instrument will strengthen the...
- The National Science Foundation awarded a $260,000 Project Grant to Rutgers, The State University of New Jersey, Camden under the Integrative Activities program (CFDA 47.083) to acquire a MALDI-TOF mass spectrometer. The mass spectrometer will be used to support multidisciplinary research and student education in fields including biomimetic self-assembly, cellular biochemistry, protein chemistry, novel biomaterials, nanomaterials, forensic analysis and molecular sensing. Specifically, it will...
- The National Science Foundation Office of Integrative Activities awarded a $1,179,143 Project Grant to the University of New Hampshire (UNH) to acquire an Orbitrap Eclipse Tribrid mass spectrometer. This equipment will (i) dramatically improve UNH's mass spectrometry capabilities to expedite diverse research projects, (ii) facilitate hands-on training and inquiry-based learning on modern instrumentation, and (iii) establish a regional high-resolution mass spectrometry facility. The funding...
- The National Science Foundation Division of Chemistry awarded $950,000 to the University of California, Berkeley under the Mathematical and Physical Sciences federal grant program (CFDA 47.049) to support research on the chemistry and physics of charged aqueous nanodrops and their applications in mass spectrometry. Professor Evan Williams and his group will investigate how the size and shape of electrospray ionization emitters affect initial droplet size and mass spectrometry performance. They...
- This National Science Foundation project grant of $237,745 awarded on September 1, 2022 through August 31, 2025 under the Mathematical and Physical Sciences program (CFDA 47.049) will support the development of a variable temperature ion trap mass spectrometer at Stony Brook University. The mass spectrometer will facilitate isomer-selective thermochemical, structural, and spectroscopic characterization of bimolecular interactions to advance research in biochemistry, medicinal chemistry,...
- This $470,000 Project Grant from the National Science Foundation (NSF) Division of Chemistry, under the Mathematical and Physical Sciences program (CFDA 47.049), supports the development of new analytical detection and processing tools for the characterization of complex mixtures, with a focus on dissolved organic matter (DOM). Researchers at Florida International University are creating a platform that combines ion mobility spectrometry and ultra-high-resolution Fourier transform ion...
- This National Science Foundation project grant of $120,813 awarded on November 1, 2022 supports research into the effects of post-translational modifications on peptide fragmentation patterns observed during tandem mass spectrometry experiments. The grantee, Illinois State University, will examine model systems relevant to MS2 under the guidance of Professor George Barnes. A combined computational and experimental approach will investigate how secondary structure and non-covalent interactions...
NOVEL ION CHEMISTRY AND INSTRUMENT DEVELOPMENT FOR THE CHARACTERIZATION OF PROTEINS, NUCLEIC ACIDS, AND HETEROGENEOUS BIO-COMPLEXES VIA TANDEM MASS SPECTROMETRY - PROJECT SUMMARY MASS SPECTROMETRY AND TANDEM MASS SPECTROMETRY HAVE BECOME ESSENTIAL TOOLS IN VIRTUALLY ALL OF THE MOLECULAR SCIENCES ASSOCIATED WITH BIOMEDICAL RESEARCH. MANY QUESTIONS OF INTEREST CAN BE ADDRESSED BY BREAKING MACROMOLECULES OF INTEREST (E.G., PROTEINS) INTO SMALLER FRAGMENTS PRIOR TO ANALYSIS. HOWEVER, INFORMATION INHERENTLY PRESENT IN INTACT MACROMOLECULES AND THEIR COMPLEXES IS LOST UPON DIGESTION, WHICH HAS MOTIVATED THE DEVELOPMENT OF SO-CALLED "TOP-DOWN PROTEOMICS" AND "NATIVE MASS SPECTROMETRY". WHILE MAJOR ADVANCES HAVE BEEN MADE IN BOTH OF THESE AREAS, SIGNIFICANT CHALLENGES REMAIN, PARTICULARLY FOR HIGH MASS HETEROGENOUS MIXTURES. THIS EFFORT EMPHASIZES NOVEL ION CHEMISTRIES AND NOVEL INSTRUMENTATION DIRECTED TO CHALLENGES IN TOP- DOWN TANDEM MASS SPECTROMETRY OF BIOPOLYMERS AND THEIR COMPLEXES. PARTICULAR EMPHASIS IS PLACED ON THE ATTACHMENT OF MULTIPLY-CHARGED IONS TO HIGH-MASS ANALYTE IONS OF OPPOSITE POLARITY TO FACILITATE MASS MEASUREMENT, MIXTURE ANALYSIS, AND STRUCTURAL CHARACTERIZATION. THE CONTROLLED ATTACHMENT OF REAGENTS OF KNOWN MASS AND CHARGE CAN DRAMATICALLY IMPROVE THE 'PEAK CAPACITY' OF A MASS SPECTROMETRY MEASUREMENT APPLIED TO COMPLEX MIXTURES OF IONS DERIVED FROM ELECTROSPRAY IONIZATION. FURTHERMORE, ION ATTACHMENT, IN CONJUNCTION WITH MSN WORKFLOWS, MAY PROVE TO BE USEFUL IN REVEALING THE SURFACE EXPOSURE OF COMPONENTS IN A COMPLEX. NOVEL ION/ION REACTIONS INVOLVING SUPERACID ANIONS MAY ALSO PROVE TO BE USEFUL AS GAS-PHASE MEANS FOR THE SELECTIVE REMOVAL OF METAL IONS THAT BECOME INCORPORATED IN BIO-COMPLEXES IONIZED UNDER NATIVE CONDITIONS. THIS EFFORT ALSO INVOLVES INSTRUMENT DEVELOPMENT AIMED AT SUPPORTING HIGH MASS-TO-CHARGE ION MANIPULATION (E.G., DIGITAL ION TRAP OPERATION FOR ION ISOLATION AND ION PARKING OF HIGH MASS-TO-CHARGE IONS) AND MEASUREMENT (DIGITAL ION TRAP OPERATION AND ELECTROSTATIC ION TRAP MASS ANALYSIS) TO SUPPORT MULTIPLY-CHARGED ION ATTACHMENT EXPERIMENTS. THE EFFORT WILL EXTEND THE UTILITY OF MASS SPECTROMETRY AND TANDEM MASS SPECTROMETRY IN TOP-DOWN BIOPOLYMER CHARACTERIZATION AS WELL AS IN THE MASS MEASUREMENT AND CHARACTERIZATION OF LARGE COMPLEXES, SUCH AS MOLECULAR MACHINES AND VIRUSES.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $365.9k | 4/9/25 | ||
| Not listed | $0 | 11/21/24 | ||
| Not listed | $0 | 11/21/24 | ||
| Not listed | $365.9k | 4/19/24 | ||
| Not listed | $365.9k | 4/19/24 |