Genetically encoded magnetometer for intracellular dynamic measurement
Active Project Grant Awarded Last action
- Award ID
- R21GM154219
- Recipient
-
The Johns Hopkins University UEI FTMTDMBR29C7
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $414,067
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date 80% complete
- Originally
- Place of performance
- Baltimore, MD 21218, USA
Award details come from USAspending.gov.
Description
- Abstract
- Mounting evidence suggests that magnetic fields may modulate important functions in cells. For example, magnetic fields can influence the spin state of photo-generated radical pairs in many flavoproteins, determining the rate of downstream reactions that govern cellular behaviors including circadian rhythms, DNA repair, and navigation of migratory birds. However, only low-to-moderate efficacy has been reported in clinical applications such as transcranial magnetic stimulation (TMS) and magnetotherapy, to treat epilepsy, depression and chronic pain. Improvement in treatment efficacy has been long stalled because the underlying molecular mechanism is poorly understood. This lack of progress is mostly due to not knowing the molecular mechanism of magnetic modulation. To measure the effective intracellular magnetic field to which biomolecules are exposed is the first step of building such understanding. The intracellular magnetic field is likely heterogeneous and not equivalent to the externally applied field of TMS or magnetotherapy, due to heterogeneous cellular compositions with varying magnetic permeabilities, as well as the heterogenous dynamic magnetic fields resulting from ION fluxes through ION channels. Currently no appropriate magnetometer exists to map the heterogeneous intracellular magnetic fields, though cellular measurement has been attempted using nitrogen a vacancy-center nanodiamond-based magnetometer, which can only provide sparse spatial readouts across the cell. Moreover, the necessity of subjecting cells to microwave radiation during nitrogen vacancy-center nanodiamond-based magnetometry is potentially harmful to cells. Herein we propose a genetically encoded magnetometer (GEM). GEM is a recombinant protein the backbone of which is a modified version of magnetosensitive flavoprotein. We will demonstrate that the heterogenous intracellular magnetic field can be mapped by expressing GEM in cells at unprecedented spatial resolution without the use of microwaves. If successful, GEM will enable the mechanistic study of TMS and magnetotherapy. In addition, IT may also aid the evaluation of biological impacts due to pervasive presence of devices emitting electromagnetic waves.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $178,481 | |
| Original award | $235,586 |
Subawards
| Subrecipient | Amount | Awarded |
|---|---|---|
| Subrecipient not on record | $41,359 |
Quick answers
Who received award R21GM154219?
R21GM154219 was awarded to The Johns Hopkins University by National Institute of General Medical Sciences (National Institutes of Health) on June 3, 2024.
How much is award R21GM154219 worth?
$414,067 has been obligated on R21GM154219.
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