This R03 project grant, awarded by the National Center for Advancing Translational Sciences (NCATS) under CFDA Program 93.350, aims to comprehensively identify metabolic enzymes that control the generation of TCF1+ progenitor CD8+ T cells, which are critical for durable anti-tumor immune responses. The $169,500 award, spanning from September 1, 2024 to August 31, 2026, will fund two key research objectives: Identifying metabolic enzymes whose loss alters T cell differentiation and the TCF1+ progenitor state across varying T cell receptor affinities, using a CRISPR/Cas9 screening approach. Candidate genes will be functionally validated through flow cytometry and metabolic analyses, with effects on anti-tumor efficacy evaluated in mouse models and human patient-derived organoids. Characterizing the transcriptomic and metabolomic profiles of TCF1+ progenitor-like cells induced by different metabolic manipulations, to determine core properties and intrinsic heterogeneity of this critical CD8+ T cell subset. The results from this project will inform future mechanistic studies and the development of a competitive R01 application, advancing the awardee's career transition from a KL2-supported scholar to an independent investigator.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $169.5k | 8/30/24 |