Project Grant R03AI183169
- This NSF Biological Sciences program award in the amount of $497,509 to the Regents of the University of Minnesota will support collaborative research on how prophages (viruses that infect bacteria) influence the structure and function of animal microbiomes. The project will utilize the invertebrate chordate Ciona robusta and the vertebrate zebrafish Danio rerio as model systems. The research will integrate genetic, predictive modeling, and experimental approaches to study the biology and...
- The National Science Foundation (NSF) awarded a $802,161 Project Grant to the University of Massachusetts Medical School (UMass Medical) to study the structural mechanisms of bacteriophage (phage) infection and assembly. This 3-year grant, effective June 1, 2024, aims to advance the understanding of virus-host interactions, promote the development of effective antiviral strategies, and leverage phage-related applications. The research will focus on three aspects of the phage life cycle: virus...
- This National Science Foundation Project Grant of $880,632 supports research into drivers and effects of latent phage activation in marine SAR11 bacteria. Funded under the Geosciences program (CFDA 47.050), this three-year award to the University of California, Los Angeles will fill critical knowledge gaps about host-virus interactions in the global oceans. Through field surveys, laboratory experiments, and mathematical modeling, the grantee will quantify the frequency of prophage infections...
- The National Institute of Allergy and Infectious Diseases (NIAID) awarded The Washington University a Project Grant of $142,492 under the Allergy and Infectious Diseases Research program (CFDA 93.855) for research spanning from November 1, 2025, through December 31, 2027. The project focuses on developing a high-throughput, single-cell RNA-sequencing (scRNA-seq) methodology to identify bacteriophage-host pairs within complex microbial communities. This research addresses a critical gap in...
- The University of South Florida (USF) received a $681,876 Project Grant from the National Science Foundation (NSF) Division of Integrative Organismal Systems under the Biological Sciences grant program (CFDA 47.074). The grant will fund a collaborative research project to investigate how prophages (viruses that infect bacteria) influence the structure and function of animal microbiomes, specifically in Ciona robusta (an invertebrate chordate) and Danio rerio (zebrafish). The project will...
- This Project Grant award from the National Science Foundation (NSF) under the Biological Sciences (CFDA 47.074) Federal Grant Program provides $987,566 to the University of California, Los Angeles (UCLA) to conduct research on viral self-assembly, from test tube to cell cytoplasm. The award period runs from December 15, 2024 to November 30, 2028. The key objectives of this project are to elucidate the differences between in vitro and in cellulo viral processes by progressively adding molecules...
- This Project Grant award from the National Science Foundation's (NSF) Division of Molecular and Cellular Biosciences, under the Biological Sciences program (CFDA 47.074), provides $833,999.00 to Georgia Tech Research Corporation (Georgia TECH) to investigate how defined microbiomes respond to viruses over short and long timescales. The project aims to test the hypothesis that robust microbiome functions are promoted by microbial communication systems and costs of virus resistance. This...
- This National Science Foundation Project Grant of $365,556 supports collaborative research between mathematicians, biologists, and engineers at the University of Wisconsin-Madison to develop new mathematical tools and biological experiments essential to understanding virus spread and extinction. Funded through the NSF's Biological Sciences program (CFDA 47.074), the research will establish an integrated experimental-mathematical framework to study how spatial structure affects viral evolution...
- The National Science Foundation (NSF) Division of Molecular and Cellular Biosciences awarded the University of Florida (UF) a $750,000 Project Grant under the Biological Sciences program (CFDA 47.074) to investigate the mechanism of superinfection exclusion (SIE) by Citrus Tristeza Virus (CTV), a plant virus model system. The project aims to elucidate the role of a viral long non-coding RNA in the SIE phenomenon, which influences viral pathogenesis and evolution. Key research activities...
- This $400,000 Project Grant award from the National Science Foundation's (NSF) Mathematical and Physical Sciences (CFDA 47.049) program supports research to model the complex dynamics of viruses and bacteria in ancient ocean-like environments. The project aims to identify "keystone" viruses that significantly influence microbial community structure and biogeochemistry in these stable, oxygen-free lake ecosystems. The research will develop innovative mathematical methods to predict...
MECHANISMS AND DYNAMICS OF SUPERINFECTION EXCLUSION IN MICROVIRAL PROPHAGE POPULATIONS - MECHANISMS AND DYNAMICS OF SUPERINFECTION EXCLUSION IN MICROVIRAL PROPHAGE POPULATIONS PROJECT SUMMARY THE MICROVIRIDAE, SMALL, SINGLE-STRANDED DNA (SSDNA) VIRUSES THAT INFECT BACTERIA ARE AMONG THE MOST ABUNDANT AND STABLE COLONIZERS OF THE HUMAN GUT. THEREFORE, COMPETITION FOR HOST CELLS BETWEEN LYSOGENIC MICROVIRUSES IS EXPECTED TO BE FIERCE. HYPERVARIABLE REGIONS (HVRS) MEDIATING PROPHAGE DEFENSE AGAINST OTHER VIRUSES (SUPERINFECTION EXCLUSION, SIEX) AS WELL AS OVERCOMING THESE DEFENSES HAVE EVOLVED NUMEROUS TIMES CONCURRENTLY WITH THE EVOLUTION OF LYSOGENY IN DIFFERENT MICROVIRAL LINEAGES. WE THUS HYPOTHESIZE THAT MICROVIRUSES HAVE ALSO EVOLVED ONE OR MORE ENTIRELY NOVEL WAYS BY WHICH THEY PREVENT VIRAL SUPERINFECTIONS, AND THAT THESE MECHANISMS HAVE PROFOUND INFLUENCE ON THEIR GENOME DYNAMICS. IN ADDITION, THERE IS EVIDENCE THAT THESE MECHANISMS MIGHT HAVE BEEN ADAPTED BY BACTERIA TO BECOME ANTI-PHAGE DEFENSE SYSTEMS. OUR PROJECT'S OBJECTIVES ARE THUS TO UNDERSTAND A) THE ORIGIN(S) OF MICROVIRAL SIEX AND B) THE INFLUENCE OF HVR DIVERSITY ON SIEX DYNAMICS. TO THIS END, WE WILL COMPARE THE BASIC MECHANISMS OF SIEX IN INDEPENDENTLY EVOLVED MICROVIRUS PROPHAGES, A PUTATIVE DEFENSE SYSTEM, AND POTENTIAL SIEX-LIKE MECHANISMS IN LYTIC MICROVIRUSES THAT MIGHT HAVE SERVED AS EXAPTATIONS IN PROPHAGE SIEX. SECONDLY, WE WILL EXPERIMENTALLY TEST THE EFFECT OF HVRS ON SIEX, IN PARTICULAR WHETHER SPECIFIC MUTATIONS ALLOW THE AVOIDANCE OF SIEX AND HOW DIFFERENT HVR TYPES INTERACT WITH EACH OTHER. THIS WILL BE DONE THROUGH SYNTHESIS OF VARIANTS OF THE MODEL MICROVIRUS ENTEROGOKUSHOVIRUS CARRYING ALL KNOWN HVR TYPES. PAIRWISE INFECTION ASSAYS WILL THEN DETERMINE WHICH HVRS CAN BLOCK OR OVERCOME SIEX OF OTHER HVRS. THIS STUDY WILL PROVIDE CONSIDERABLE INSIGHTS INTO THE BIOLOGY OF THE MICROVIRIDAE AND ALSO INSIGHTS INTO THE INFLUENCE OF SIEX ON THE POPULATION DYNAMICS OF PROPHAGES IN GENERAL. ADDITIONALLY, IT WILL CONTRIBUTE TO OUR GROWING KNOWLEDGE OF THE ARSENAL OF PHAGE DEFENSE MECHANISMS IN BACTERIA.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $0 | 2/3/26 | ||
| Not listed | $71.5k | 4/7/25 | ||
| Not listed | $71.5k | 4/7/25 | ||
| Not listed | $71.7k | 2/20/25 | ||
| Not listed | $71.7k | 2/20/25 |