Project Grant R01NS146952
- The National Institute of Neurological Disorders and Stroke awarded Trustees of Boston University $595,774 on June 1, 2026, under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853) to develop far-red microscopy techniques and genetically engineered voltage indicators for deep-tissue imaging of large neuronal populations in the mouse brain. The project develops two complementary microscope systems optimized for high-speed voltage imaging at...
- The National Institute of Mental Health awarded the University of Washington a cooperative agreement totaling $2,305,031 on March 9, 2026, to engineer red fluorescent calcium and neuromodulator sensors for multiplexed neuronal imaging. This work is funded under the Mental Health Research Grants program (CFDA 93.242). The project leverages the Opto-Mass sensor screening platform and machine learning models to accelerate fluorescent sensor development, addressing limitations in dynamic range,...
- The National Institute of Neurological Disorders and Stroke awarded Massachusetts Institute of Technology $651,069 on May 2, 2026, under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853) to develop spatially multiplexed imaging techniques for visualizing complex signaling networks in neurons and brain cells. The research advances a method called Signaling Reporter Islands (SIRIs) that enables simultaneous imaging of multiple cellular signals...
- The National Institute of General Medical Sciences awarded the University of Washington $2,102,360 on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop ultrahigh multiplexing fluorescent probes and methods for spatial transcriptomics of tissue samples. The funded work encompasses design, synthesis, and characterization of over 1,000 spectrally and DNA-barcoded fluorescent probes conjugated with DNA oligonucleotide barcodes to enable single-molecule...
- The National Institute of General Medical Sciences awarded the University of Washington $891,007 on June 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop multiplexed optical sensors for redox profiling in human induced pluripotent stem cell models of disease and drug response. The funded research program develops next-generation, multiplexed optical platforms for quantitative redox phenotyping and applies them to disease modeling and drug screening in...
- The National Institute of Neurological Disorders and Stroke awarded William Marsh Rice University $105,652 on September 10, 2026, under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853) to develop genetically encoded voltage indicators (GEVIs) optimized for low-phototoxicity widefield one-photon imaging of neuronal activity. The project addresses a critical need for tools that capture rapid voltage changes underlying neuronal signaling without...
- The National Institute of Neurological Disorders and Stroke awarded Vanderbilt University $557,161 on July 10, 2026, under the Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative (CFDA 93.372) to develop a novel bioluminescence resonance energy transfer (BRET) system for optimizing and imaging in vivo recording of neural activity in freely behaving animals. The project runs through June 30, 2029, with performance in Nashville, Tennessee. The award supports...
- The National Institute of General Medical Sciences awarded Seattle Children's Research Institute $2.096 million on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop an AI-driven platform for generating conditionally stable nanobodies for intracellular detection and protein manipulation applications. The project spans four years through April 30, 2030, and will deliver three core outputs. First, an expanded AI-based nanobody stability prediction...
- The National Institute of Neurological Disorders and Stroke awarded Rosalind Franklin University of Medicine & Science $396,015 on January 1, 2026, under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853) to support molecular characterization of genes regulating CAV2 voltage-gated calcium channels and their roles in channel trafficking and degradation. The research uses C. elegans as a model organism to identify and characterize genes...
- The National Institute of General Medical Sciences awarded the University of Washington $380,371 on March 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop computational methods that infer cellular history from single-cell sequencing data, enabling analysis of disease progression in tissues that cannot be sampled longitudinally, such as the brain in Alzheimer's disease or the heart in cardiomyopathy. The research deploys three integrated approaches:...
The National Institute of Neurological Disorders and Stroke awarded the University of Washington $676,113 on September 1, 2026, under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853) to develop improved red genetically-encoded calcium indicators (GECIs) for neural activity monitoring. The award funds machine-learning-guided protein engineering to overcome performance limitations in current red fluorescent GECIs such as JRCaMP1 and JRGECo1, which suffer from reduced brightness, dynamic range, kinetics, and photostability compared to green counterparts and exhibit blue-light photoactivation artifacts complicating multiplexed imaging with optogenetic tools. The project pursues three integrated aims: machine-learning-driven optimization to predict single-site mutations improving brightness, calcium sensitivity, decay kinetics, and dynamic range, trained on approximately 1,000 characterized variant datasets; construction and high-throughput screening of combinatorial mutational libraries at up to four key sites to generate synergistic mutation combinations; and in vivo validation of optimized red GECIs using two-photon and three-photon microscopy in awake and anesthetized preparations. The work is performed in Seattle, Washington, with a period of performance extending from September 1, 2026, through May 31, 2031.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $676.1k | 8/31/26 |