This Project Grant award, valued at $497,870 and provided by the National Cancer Institute (CFDA 93.394 - Cancer Detection and Diagnosis Research), aims to develop a novel genetically-encoded molecular imaging approach for tracking genome-edited immune cells in vivo. The project will leverage bacterial metallophores as contrast agents to non-invasively monitor the distribution, turnover, and survival of these engineered cells using positron emission tomography (PET) imaging. The first phase will focus on developing and evaluating the mammalian expression vector for the bacterial transporter protein, which will then be incorporated into CAR-T cells for in vivo reporting. The second phase will assess the in vivo pharmacokinetics, biodistribution, and PET imaging of the metallophore/radioisotope probes and genome-edited cells in animal models. If successful, this innovative strategy could enable precise, real-time tracking of genetically-modified immune cells, which is critical for advancing cell-based therapies. The project includes a $497,870 sub-award to the Children's Hospital Medical Center in Cincinnati to support this research.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $497.9k | 9/17/24 |
Grant Number | Description | Subgrantee | Prime Award | Dollars Obligated (Click to sort descending) | Updated At (Click to sort ascending) |
|---|---|---|---|---|---|
01639900002S | Childrens Hospital Medical Center | Project Grant R01CA279962 | $14.6k | 4/15/25 |