This $987,578 federal Project Grant award from the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) supports research to understand how receptor tyrosine kinase (RTK) proteins mediate cellular signaling in response to diverse extracellular ligands. The primary objectives are to: 1) acquire comprehensive data on ligand-specific potencies, efficacies, and signaling preferences for the epidermal growth factor receptor (EGFR), 2) perform a physical-chemical...
This federal Project Grant award of $452,192 from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) supports the development of pan- and phospho-specific nanobodies for investigating MAPK and PP2A signaling at Vanderbilt University. The goal of this 4-year research project, commencing on June 1, 2025, is to advance the understanding of the reversible protein phosphorylation processes that control cellular homeostasis. The...
This $213,284 Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) under CFDA Program 93.859 (Biomedical Research and Research Training) supports research by Yale University to investigate how the cell membrane environment impacts the structure and function of the epidermal growth factor receptor (EGFR), a receptor tyrosine kinase that plays a crucial role in cellular processes and cancer development. The key objectives are to: 1) Use kinetic assays and defined...
The University of Texas Southwestern Medical Center was awarded a $574,000 Project Grant from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859). The 5-year grant, effective May 1, 2025 through February 28, 2030, will support the center's research on understanding the general principles of receptor tyrosine kinase (RTK) signaling through structural and functional studies. The research aims to elucidate how...
This Project Grant award from the National Cancer Institute (CFDA 93.396 Cancer Biology Research) to Vanderbilt University provides $335,944 from September 1, 2024 to August 31, 2027 to further develop, refine, and validate the university's proprietary free-solution assay (FSA) technology and compensated interferometric reader (CIR) platform. The FSA-CIR system represents a novel, label-free, solution-phase, ultra-sensitive, and enzyme-free technology that can quantify molecular interactions...
This federal Project Grant award from the National Cancer Institute (CFDA 93.396 Cancer Biology Research) provides $391,531 to Yale University to develop technology for directing phosphorylation of specific protein kinase substrates. The goal is to engineer a kinase mutant that can selectively phosphorylate a designer allele of one of its substrates, using knowledge of kinase phosphorylation site specificity. The researchers will establish the feasibility of this approach using the tumor...
This federal Project Grant award of $449,196 from the National Cancer Institute (CFDA 93.395 Cancer Treatment Research) supports research at The University of Texas Southwestern Medical Center to investigate mechanisms of resistance to tyrosine kinase inhibitor (TKI) therapy in cancer. The research aims to determine how TKI treatment induces rapid sumoylation (a type of post-translational modification) of receptor tyrosine kinases (RTKs), which changes their function from kinases to adaptor...
This federal Project Grant award, worth $630,000.00, was provided by the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) to the University of New Mexico (UNM). The grant will support the development of an advanced optical microscopy system that combines recent breakthroughs in imaging techniques, including MINFLUX and modal imaging. This combined system will enable the observation of fast protein interactions and dimerization kinetic rates within the living cell...
This Project Grant award, provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859), aims to resolve the interaction network of cell surface receptors and quantify their dynamics in live cells. The $733,870 award to Texas Tech University System will fund research focused on: (1) investigating how ligand binding and cellular environment affect the local network of receptor tyrosine kinase (RTK) interactions,...
This Project Grant award from the National Cancer Institute (CFDA 93.396 - Cancer Biology Research) will support the development of a novel approach to detect and report on the expression of multiple cytokine genes simultaneously in tissues. The $234,919 award to Yale University, to be completed by August 31, 2027, will combine CRISPR-mediated transcriptional activation with genetically-encoded fluorescent reporters to enable the dynamic, high-sensitivity and multiplexed monitoring of cytokine...