Project Grant DP2HD125963
- The National Institute of General Medical Sciences awarded $2.164 million to The Regents of the University of California, San Francisco on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop a fluorescent protein optical barcode CRISPR platform for genome-wide image-based phenotypic screening. The project will create novel CRISPR libraries using fluorescent proteins as barcodes to identify genetic perturbations in live cells and develop a...
- The National Institute of General Medical Sciences awarded The Regents of the University of California (UC Riverside) $420,520 on July 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to conduct mechanistic studies of CRISPR-Cas9 genome editing using molecular simulations and AI-driven approaches. The research integrates physics-based molecular simulations with artificial intelligence methods to advance understanding of CRISPR-Cas9 conformational dynamics,...
- The National Institute of Biomedical Imaging and Bioengineering awarded University of California, Berkeley $120,394 on May 1, 2026, under the Discovery and Applied Research for Technological Innovations to Improve Human Health program (CFDA 93.286) to develop genome editing delivery vehicles that amplify editing efficiency by enabling cells to produce and distribute CRISPR-Cas9 editing enzymes to neighboring cells in vesicles. The research addresses inefficient delivery of genome editing to cell...
- The National Institute of General Medical Sciences awarded the University of California, San Diego $526,805 on July 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop episomal single-cell reporter assays for measuring the activity of hundreds to thousands of regulatory elements with tissue-specific resolution in whole developing embryos. The research focuses initially on enhancers as critical regulatory elements controlling gene expression timing and...
- The National Institute of General Medical Sciences awarded The Regents of the University of California, San Francisco $562,484 on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop next-generation visualization and analysis software for multiscale molecular modeling. The recipient will create cutting-edge software tools that integrate visualizations and analyses of molecular structures and related biological information, including...
- The National Institute of General Medical Sciences awarded the University of California, Los Angeles $433,125 on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop imaging-based molecular recording methods that reconstruct the signaling history of individual cells during development. The funded research extends a signal-recording approach that uses regulated CRISPR base editors to generate mutations in engineered genomic target sites at rates...
- The National Institute of Neurological Disorders and Stroke awarded The Leland Stanford Junior University $618,680 on August 11, 2026, under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853) to develop ultrasound-controlled in vivo gene editing with photoswitchable CRISPR-CasMini technology. The project aims to enable noninvasive, cell-type-specific gene editing in the brain by combining two engineering innovations: a single-chain...
- The National Institute of General Medical Sciences awarded the University of California Santa Cruz $425,033 on July 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to map variant histone protein interactions and elucidate the molecular mechanisms governing those interactions through advanced proteomics and structural biology. Over the five-year performance period (July 1, 2026 through April 30, 2031), the principal investigator will use nucleosome...
- The National Institute of General Medical Sciences awarded The Regents of the University of California, San Francisco $445,049 on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop membrane-inspired lipid nanoparticles for cell-type-specific expression of programmable RNA. The recipient will design novel lipid nanoparticle formulations to achieve precise, scalable delivery of biomolecules to specific cell types, combining bio-inspired...
- The National Institute of Biomedical Imaging and Bioengineering awarded The Trustees of Princeton University $597,419 on August 15, 2026, under the Discovery and Applied Research for Technological Innovations to Improve Human Health program (CFDA 93.286) to develop scalable and deployable multiplexed nucleic acid detection using CRISPR-based Cas13 technology. The research combines miniaturized CRISPR-based nucleic acid detection with microfluidic systems to enable highly multiplexed pathogen,...
The National Institutes of Health Office of the Director awarded The Regents of the University of California, San Francisco $779,000 on September 1, 2026, under the Trans-NIH Research Support program (CFDA 93.310) to develop CRISPR-CHROMEXM, a next-generation imaging platform for direct visualization of single-molecule gene regulation during vertebrate embryogenesis. The project will integrate catalytically inactive Cas9 with the existing ChromExM (chromatin expansion microscopy) method to enable sequence-resolved 3D visualization of chromatin structure, transcriptional activity, and regulatory factor localization within embryos at single-nucleosome resolution using standard confocal microscopes. The recipient will apply this platform to investigate heterochromatin emergence during exit from pluripotency in vertebrate embryos and functionally test the role of histone modifications in driving chromatin compaction and transcriptional repression. Deep learning-based image analysis will reveal how chromatin organization regulates gene activity during cell-fate transitions. The work aims to establish CRISPR-CHROMEXM as a broadly accessible platform for molecular-resolution imaging of gene regulation in vivo. Performance occurs in San Francisco, California with a period of performance from September 1, 2026, through May 31, 2031.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $11.9k | 8/28/26 | ||
| Not listed | $0 | 8/27/26 | ||
| Not listed | $767.1k | 8/26/26 |