This $1,539,200 Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) supports the development of an integrated technology platform to enhance the characterization of functional oligonucleotides. The award will fund the creation of a front-end ionization technique and microfluidics device to dramatically improve the sensitivity and structural resolution of mass spectrometry analysis for nucleic acids....
This $1,035,125 Project Grant award from the National Institutes of Health (NIH) Research Infrastructure Programs (CFDA 93.351) will fund the acquisition of a NovaSeq X Plus high-throughput DNA sequencing system at the Dana-Farber Cancer Institute (DFCI) Molecular Biology Core Facilities (MBCF). The new sequencing instrument will complement DFCI's existing Illumina sequencing infrastructure, enabling cost-effective and rapid delivery of whole genome sequencing, methylation profiling, single-cell...
This Project Grant award, valued at $394,857.00, was provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859). The grant supports the development of a system to protect and concentrate large DNA molecules for genome analysis, with the goal of enabling more comprehensive study of structural variations associated with various diseases. The key products and services to be delivered under this award include: 1)...
The National Human Genome Research Institute (NHGRI), through its Human Genome Research Federal Grant Program (CFDA 93.172), awarded a $2,040,824 Project Grant (R01HG013861) to the University of Massachusetts (UMass) to develop an enzymatic system for site-specific incorporation of modified RNA bases. The research aims to build on advances in co-immobilized flow transcription to enable the synthesis of RNA with position-specific substitutions, facilitating studies of RNA structure, dynamics, and...
This $1,675,000 five-year Project Grant award from the National Institute of General Medical Sciences (NIGMS) under CFDA Program 93.859 (Biomedical Research and Research Training) supports two key research areas at the University of Massachusetts Medical School (UMass Medical): Investigating how the tumor marker protein Ki-67 protects chromosomes from DNA damage during mitosis, including mapping the specific genome locations impacted, determining the critical amino acid residues involved, and...
This Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), provides $406,500 to Directseq Biosciences, Inc. to advance high-throughput mass spectrometry-based sequencing (NGMS-SEQ) methods for direct sequencing and quantitative mapping of RNA modifications. The key objectives are to: 1) enhance NGMS-SEQ techniques to enable high-throughput direct sequencing of various tRNA samples, including physiologically...
This Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), is for the development of an exhaustive de novo RNA sequencing method using next-generation mass spectrometry (NGMS-seq). The $215,088 award to Directseq Biosciences, Inc. aims to 1) further advance NGMS-seq to completely sequence all RNA species in a sample, including comprehensive identification and quantification of RNA modifications, and 2) test the...
This federal Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) provides $230,312.00 in funding to the University of Massachusetts Medical School (UMass Medical) to conduct research investigating RNA interference and related gene regulatory mechanisms over evolutionary time. The research aims to explore three key questions: 1) how cells distinguish "licensed" from potentially dangerous...
The University of Massachusetts Medical School was awarded a $591,414 Project Grant from the National Science Foundation Division of Molecular and Cellular Biosciences under the Biological Sciences federal grant program (CFDA 47.074) for the period February 1, 2023 through January 31, 2028. The grant will support research to uncover the regulation of low fidelity mRNA splicing through novel genomics and computational tools to identify and quantify erroneously spliced RNAs. This will provide a...
The National Human Genome Research Institute (NHGRI) awarded a $409,699 Project Grant (CFDA 93.172 - Human Genome Research) to the Regents of the University of Michigan to develop integrated LC-MS/MS and nanopore sequencing platforms for the direct and quantitative detection of RNA modifications. This collaborative project involves sub-awards to the University of Oregon and Northeastern University to contribute expertise in nanopore sequencing, RNA purification, and computational analysis. The...