Project Grant DP2GM140937

This Project Grant award from the National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) provides $452,375 to The Trustees of Columbia University in the City of New York to develop a platform for rapid and efficient generation of multi-site sequence variants using template-guided gene synthesis. The project aims to advance this synthesis approach by optimizing predictive algorithms, experimental protocols, and robotic automation to produce kilobase-sized DNA constructs up...

This Project Grant award from the National Institutes of Health's National Human Genome Research Institute provided $768,678 to Molecular Assemblies, Inc. to develop a fully enzymatic oligonucleotide synthesis cycle using engineered template-independent polymerases and a novel phosphate dNTP blocking group. The award supports the goals of the Human Genome Research program (CFDA 93.172) to accelerate genome research and its application to human health. Specifically, the awardee will engineer...

The National Institutes of Health, through the National Institute of General Medical Sciences (CFDA 93.859 Biomedical Research and Research Training), awarded a $1,638,033 Project Grant to the Scripps Research Institute in La Jolla, California. The purpose of this grant is to develop a CRISPR-free mammalian recombineering system that enables large-scale DNA insertions, deletions, and substitutions without the introduction of double-strand breaks or DNA scars. This technology has the potential to...

The National Human Genome Research Institute (NHGRI) awarded a $650,625 Project Grant (CFDA 93.172 - Human Genome Research) to The Broad Institute, Inc. to develop a novel platform called Helicase-Assisted Continuous Evolution (HACE) for performing multiplexed locus-specific hypermutation within the mammalian genome. This platform will utilize a nick-directed helicase fused to a deaminase domain to enable continuous mutagenesis across large genomic regions. The HACE system will be directed to...

The Massachusetts General Hospital will receive $3,024,000 through a three-year Project Grant from the National Institutes of Health Trans-NIH Research Support program to develop scalable methods for engineering and characterizing novel CRISPR-associated protein variants. The goal is to address limitations of current CRISPR-Cas enzymes by optimizing machine learning-guided directed evolution to create a catalog of customizable DNA editors with improved properties like expanded target...

The National Human Genome Research Institute (NHGRI) awarded a $400,000 Project Grant (CFDA 93.172 - Human Genome Research) to the University of Washington to develop new single-cell multi-omic technologies for studying the relationship between three-dimensional genome organization and gene expression. Specifically, the grant will fund the development of CARE-Seq, a novel method to jointly measure whole-genome chromatin interactions and gene expression in the same single cells from complex...

This Project Grant award from the National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) aims to develop a revolutionary DNA sequencing platform that surpasses existing technologies in accuracy and cost. The $384,418 award to Xgenomes Corp. will fund the development of a super-resolution single-molecule sequencing method that can read short sequence "words" rather than single nucleotides, enabling highly accurate and cost-effective genomic analysis. The...

This federal Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), provides $2,280,602 to Boston Children's Hospital to develop and refine computational tools to advance the safety and efficacy of genome editing. The key products and services to be delivered include: 1) Enhancing the CRISPRME tool to better predict off-target effects of genome editing across diverse human genomes, 2) Developing a new tool called...

The National Human Genome Research Institute (NHGRI) awarded a $224,101 Project Grant (CFDA 93.172 - Human Genome Research) to The Washington University to develop novel in vivo high-throughput technologies to study gene regulatory elements and disease-associated variants in mice. The key products and services to be delivered under this 4-year project grant include: Developing MPRA (Massively Parallel Reporter Assay) technology in mice using AAV6 virus and PiggyBac transposase system to...

This Project Grant from the National Science Foundation's Technology, Innovation, and Partnerships program totaling $100,000 will support research to develop next-generation clustered regularly interspaced short palindromic repeat (CRISPR) systems for curing inherited diseases. Specifically, the awardee, the University of California, Berkeley, will work to circumvent in vivo delivery challenges with CRISPR-Cas9 by developing miniature RNA-guided genome editors smaller than Cas9 that can be...