This Project Grant award from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) provides $448,150 in funding to the University of Massachusetts (UMass) to develop a general approach for rapidly modulating specific protein-protein interactions (PPIs) on living cell membranes. The key objectives are to establish a modular antibody- and aptamer-directed proximity labeling method for targeted DNA barcoding of cell membrane proteins, particularly human receptor...
This five-year $385,645 Project Grant from the National Science Foundation's Biological Sciences program (CFDA 47.074) will fund research investigating RNA-guided nucleases encoded by transposons across the three domains of life. The grantee, Columbia University, will examine an ancestral family of transposon-encoded enzymes that use guide RNAs to target and cut matching DNA sequences. These enzymes are directly related to CRISPR-Cas9 and CRISPR-Cas12 genome editing tools. The project aims to...
This $1,735,683 Project Grant award from the National Science Foundation's Biological Sciences program (CFDA 47.074) supports research aimed at determining the three-dimensional structure of the bacterial cell division protein FtsZ. The research will use advanced solid-state nuclear magnetic resonance spectroscopy (NMR) methods to study the structure, dynamics, and drug-binding capabilities of the FtsZ protein, which is considered a prime target for new antibiotic development. The project will...
The National Science Foundation Division of Molecular and Cellular Biosciences awarded a $785,201 Project Grant to The Regents of the University of Colorado to support research titled "Collaborative Research: Understanding Protein Mechanical Stability and Its Impact on Secretion." The two-year grant running from January 15, 2022 to December 31, 2024 will fund research under the Biological Sciences program (CFDA 47.074). The Biological Sciences program aims to promote progress in the...
The National Science Foundation (NSF) awarded a $740,797 Project Grant under the Biological Sciences federal grant program (CFDA 47.074) to the Regents of the University of California, San Francisco (UCSF) to develop and test new methodologies for the rational design of minimalistic light-switchable single-domain proteins. The goal is to engineer these proteins to bind specifically to target proteins in only one photoswitchable chromophore state, allowing for precise spatial and temporal control...
This $431,542 Project Grant from the National Science Foundation's Division of Chemical, Bioengineering, Environmental, and Transport Systems, under the Engineering (47.041) federal grant program, will fund research at Columbia University to define kinase-driven cellular response networks using single-cell genomics techniques. Over a five-year period from February 2022 to January 2027, the university will leverage high-throughput single-cell genomic methods to map the molecular changes cells...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) in the amount of $1,102,648 will support research to understand how dynamic molecular interactions of RNA binding proteins (RBPs) drive post-transcriptional gene expression changes during embryonic stem cell differentiation. The awardee, Columbia University, will use novel parallel approaches to globally map protein-protein interactions for...
This Project Grant award from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) provides $1,200,000 in funding to The Trustees of Columbia University in the City of New York to develop a time-resolved cryo-electron microscopy (cryo-EM) method and apply it to study the dynamics of diverse biological molecules. The proposed research seeks to enable visualization of the progression of biochemical reactions by capturing a sequence of intermediate states, similar to...
This Project Grant award from the National Cancer Institute (CFDA 93.396 Cancer Biology Research) provides $449,726 to the University of Illinois from January 2, 2025 to December 31, 2027 to develop novel tools that will enable the rapid and sensitive analysis of changes in protein interactions within living cells. The proposed approaches leverage light-regulated or engineered biotin ligases to dramatically reduce background signals and enable protein labeling within one minute or less. This...
This $300,000 EAGER (Early-concept Grants for Exploratory Research) project grant, awarded by the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074), will develop a novel experimental technique to selectively deplete specific proteins at unique genomic locations within cells. The proposed research aims to combine genomic technologies to first deplete the RDC protein complex at specific loci flanking transposable elements in Drosophila, and then extend the method to...