Project Grant 2204126

Award Date 7/1/22
Completion Date 6/30/25
Dollars Obligated $1.4M
Federal Grant Program
47.049
Assistance Type
Project Grant
Place of Performance
Knoxville, TN 37996, USA
Similar Awards
The National Institute of General Medical Sciences (NIGMS) awarded a $344,400 Project Grant (CFDA 93.859 - Biomedical Research and Research Training) to The University of Texas Southwestern Medical Center to develop new tools and approaches for improving cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) imaging techniques. Specifically, the grant aims to: 1) Develop a sample preparation strategy to reduce electron-induced radiolysis of frozen-hydrated biological...
This is a $260,138 federal Project Grant awarded by the National Science Foundation (NSF) under the Mathematical and Physical Sciences program (CFDA 47.049). The grant was awarded to Virginia Polytechnic Institute & State University (Virginia Tech) on September 1, 2024, with a projected completion date of August 31, 2027. The grant funds collaborative research to understand how variations in the molecular composition of lipids and sterols govern the material properties and emergent...
This $1,444,500 Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) aims to develop new cryogenic electron microscopy (cryo-EM) methods to visualize the structural heterogeneity of ribosomes at the single-molecule level in cells. The award, effective September 2, 2024 through August 31, 2027, will enable the principal investigator to pursue strategies to extend a novel technique called 2D template...
This two-year, $219,836 National Science Foundation Integrative Activities Project Grant supports training in cryogenic electron microscopy (cryo-EM) for researchers at the University of Nebraska-Lincoln. The grant enables a research team to acquire hands-on cryo-EM training from experts at the Pacific Northwest Center for Cryo-EM, a user facility operated by Oregon Health & Science University and Pacific Northwest National Laboratory. Upon completing the training, the researchers will...
This $299,396 National Science Foundation project grant supports research into the role of lipids in modulating synaptic plasticity of ion channels. Funded under the Biological Sciences program (CFDA 47.074), the two-year award to the University of Tennessee will elucidate the microscopic mechanisms involved in ion transport across membrane-associated protein channels. Researchers will focus on dynamic and structural features of biological membranes and leverage non-invasive scattering...
The National Science Foundation (NSF) awarded a $1,200,000 Project Grant under the Biological Sciences (CFDA 47.074) federal grant program to The Trustees of Columbia University in the City of New York. The grant supports the development of time-resolved cryo-electron microscopy (cryo-EM) techniques to study the dynamics of diverse biological molecules like RNA polymerase, GLP1R, and the AcrB multi-drug efflux transporter. The research aims to expand the application of this method beyond the...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training program (CFDA 93.859) provides $792,584 to Old Dominion University and its affiliated research foundation to develop multi-resolution docking methods and computational modeling tools for cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET). The 5-year project aims to advance flexible fitting and refinement of AlphaFold2 models, improve...
This Project Grant award of $410,939 from the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) supports research at Clemson University to elucidate the biophysical mechanisms of membrane protein folding and stability. The project will use advanced single-molecule force spectroscopy techniques, such as atomic force microscopy, to probe how membrane proteins interact with cell membranes, maintain proper orientation, and achieve their final folded state during...
The National Science Foundation (NSF) awarded a $1,200,000 Project Grant under the Biological Sciences program (CFDA 47.074) to the University of Washington to conduct research on liquid-liquid phase separation in lipid membranes. The 4-year project aims to: 1) test if asymmetric lipid vesicles phase separate similar to flat membranes; 2) develop new methods to determine lipid ratios in coexisting membrane phases and analyze lipid compositions of vesicles; and 3) use Raman imaging and...
This federal Project Grant award of $600,000 from the National Science Foundation's (NSF) Mathematical and Physical Sciences Program (CFDA 47.049) supports research by Markus Deserno of Carnegie Mellon University to investigate how the asymmetry of cell membranes affects the phase behavior of their lipid constituents. The research aims to develop theoretical and computational techniques to elucidate how factors like differential stress and cholesterol partitioning impact lipid mixing in...

This three-year, $1,368,422 project grant from the National Science Foundation's Mathematical and Physical Sciences program (CFDA 47.049) supports the development of cryogenic electron microscopy (cryo-EM) to study phase separation in lipid membranes. Frederick A. Heberle of the University of Tennessee and M. Neal Waxham of the University of Texas Health Science Center at Houston will optimize cryo-EM imaging techniques to visualize nanoscale lipid raft domains in cell membranes. Goals include determining the minimum detectable raft size, developing freely available analysis tools, and training students. A $273,688 subaward to UT Health supports all aspects of cryopreservation, cryo-imaging, cryo-tomography, and initial image processing led by Dr. Waxham to integrate his work with Dr. Heberle's analysis programs. Insights on lateral heterogeneity in complex lipid-protein mixtures could further understanding of cell membrane organization and function.

Generated 1/7/24, 2:32 AM