Awards issued prior to january 20, 2025, were funded under previous administrations and may not reflect the priorities and policies of the current administration.** we propose to develop a dynamic…

Awards issued prior to january 20, 2025, were funded under previous administrations and may not reflect the priorities and policies of the current administration.** we propose to develop a dynamic risk model and climate suitability model based on existing climate data and current observational/experimental data to be generated through these research objectives: (1) document the distribution of HLB and ACP in cold-hardy citrus growing regions of south georgia north florida and east-central california (2) evaluate preference of ACP to cold-hardy citrus cultivars (3) determine the titer of clas in trees following exposure to cold and freezing events and (4) tolerance and acclimatization in ACP populations to cold and determine underlying mechanisms in the insect's body to these adaptations. The proposal addresses three priority areas stated by the citrus disease subcommittee (CDS) and ecdre program: (#2) regional management or eradication of ACP (#3) predictive models of psyllids movement and dispersal early detection of HLB and (#9) greater understanding of the ecology and interactions of the citrus production system and HLB disease complex. Citrus production acreage in areas with cool winter temperatures is increasing and the sustainability of cold-hardy citrus production in parts of california florida and georgia. The lack of scientific studies on the effect of cold on both clas and ACP populations leaves cold-hardy citrus production in US at risk and must be addressed. Our proposed objectives will provide basic information on the influence of cold on vector and pathogen biology which would help in developing region-specific risk models to help in decision-making process for effective sampling surveillance and future expansion of groves.