Brain connects: high-throughput single cell projection mapping and spine quantification in the primate brain by synaptic barcoding
Active Cooperative Agreement Awarded Last action
- Award ID
- U01NS136398
- Recipient
-
The Broad Institute, Inc. UEI H5G9NWEFHXN4
- Awarding agency
-
National Institute of Neurological Disorders and Stroke
National Institutes of Health, Department of Health and Human Services
- Obligated
- $4,265,169
Award facts
- Assistance listing
- 93.279 Drug Use and Addiction Research Programs
- Completion date 74% complete
- Place of performance
- Cambridge, MA 02142, USA
Award details come from USAspending.gov.
Description
- Abstract
- The primate brain contains trillions of highly specific synaptic connections that are the building blocks of neural computation. While tools available in rodents have begun to shed light on how particular cell types and circuits participate in brain functions, these approaches are not currently possible to apply to primates. Recently, our labs have developed technology that: 1) delivers barcoded rnas to the presynaptic and postsynaptic compartments with high sensitivity and specificity using aavs; and 2) allows CNS-wide transduction by AAV through systemic injection. We will combine these innovations, together with our platforms for single-cell and spatial transcriptomics, to systematically map the projections of thousands of midbrain dopaminergic neurons across the marmoset brain. In addition, we will develop strategies for the rapid and scalable quantification of postsynaptic spines in individual cells, using known abundance in spine variation amongst the cells of the dentate gyrus as a validation. Together, these technologies will enable the routine quantification of presynaptic and postsynaptic phenotypes at a level of resolution and scale that has hitherto been impossible in primate
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $803,786 | |
| Amendment | $0 | |
| Amendment | $723,408 | |
| Amendment | $1,130,403 | |
| Amendment | $803,786 | |
| Original award | $803,786 |
Subawards
| Subrecipient | Amount | Awarded |
|---|---|---|
| Massachusetts Institute Of Technology | $231,527 | |
| Massachusetts Institute Of Technology | $333,986 |
Quick answers
Who received award U01NS136398?
U01NS136398 was awarded to The Broad Institute, Inc. by National Institute of Neurological Disorders and Stroke (National Institutes of Health) on August 31, 2024.
How much is award U01NS136398 worth?
$4,265,169 has been obligated on U01NS136398.
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