Self-administrated multiplex radiation indicator (samurai) kit for field triage biodosimetry

Active Cooperative Agreement Awarded Last action

Award ID
U01AI189429
Recipient
Awarding agency
Obligated
$1,081,320

Award facts

Assistance listing
93.310 Trans-NIH Research Support
Completion date 25% complete
Funding agency
Office of the Director National Institutes of Health, Department of Health and Human Services
Place of performance
Phoenix, AZ 85004, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
After a mass-casualty radiological or nuclear accident, the first triage will need to distinguish exposed from non- exposed individuals to organize the countermeasure response. In this objective, there is a need to develop a bioassay that can screen tens or hundreds of thousands of individuals in a timely manner directly on field. Our team identified radiation responsive proteins and transcripts in leukocytes that can discriminate samples irradiated with dose < 2 GY from ≥ 2 GY. In this application, we propose to design, develop, and test a "sample- in-answer-out" radiation exposure assay, named self-administrated multiplex radiation indicator (samurai) kit. The samurai kit is a self-administered, unpowered, and cost-efficient point-of-care paper-based vertical flow immunoassay (VFI) system based on a safe "tube and cap" user interface that allows the isolation and multiplex detection of both proteins and RNA from leukocytes. The handheld platform will consist of a sample preparation module (SPM) coupled to a detection (VFI) module and will be able to process sample from collection to data reporting in less than 30 min. To achieve this goal, we propose the following aims: #1) develop biodosimetry protein and transcript bioassays for paper-based format. This will involve the design and testing of sandwich immunodetection for proteins and the development of an assay chemistry allowing transcript detection using an amplification-free approach based on the detection of DNA:RNA hybrids; #2) design and test a SPM to extract proteins and gene transcripts from leukocytes. The SPM will be designed to load fingerstick-collected whole blood, lyse red blood cells, capture leucocytes by anti-CD45 antibodies and isolate RNA and proteins; #3) fabricate a VFI module to process sample directly from the SPM and generate a quantifiable signal on a multiplex paper membrane to assess irradiation status. A capillary-driven pre-existing VFI module will be adapted to fit to the SPM and transfer sample through the reaction pads and to the VFI multiplex membrane to generate a visible colorimetric signal that could ultimately be captured with commercial smartphones and analyzed by our verifast software APP. The ability to detect simultaneously protein and RNA biomarkers will make the samurai kit useful, not only for the preliminary triage during radiobiological accident, but for a large variety of applications, broadening its appeal to industry partners.

Transactions

Action Obligated Date
Amendment $539,267
Original award $542,053

Subawards

Subrecipient Amount Awarded
The Trustees Of Columbia University In The City Of New York Self-administrated Multiplex Universal Radiation Indicator (Samurai) Kit for Field Triage Biodosimetry $195,607

Quick answers

Who received award U01AI189429?

U01AI189429 was awarded to University Of Arizona by National Institute of Allergy and Infectious Diseases (National Institutes of Health) on August 1, 2025.

How much is award U01AI189429 worth?

$1,081,320 has been obligated on U01AI189429.

Which agency awarded U01AI189429?

National Institute of Allergy and Infectious Diseases (National Institutes of Health) awarded U01AI189429, with funding from Office of the Director (National Institutes of Health).

On GovTribe

See this award's full record

  • Every transaction and subaward
  • The recipient's other awards and teaming partners
  • Similar grant awards
  • Ask GovTribe AI about this award
Try for free Login