Ultrahigh throughput Sequencing system
INTRODUCTION This is a Small Business Sources Sought (SBSS) notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability and type of qualified Small Business companies technically capable of meeting this potential government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. The notice is strictly for research and information purposes only. The information requested will assist the Government in determining the appropriate acquisition method including small business socio-economic set-aside possibilities and to determine the availability of qualified Small Business companies technically capable of meeting the Government's potential requirement. All Small Business companies with the capability and availability to perform the requirement under the applicable NAICS code are invited to submit a response to this notice. NORTH AMERICAN CLASSIFICATION SYSTEM (NAICS) CODE The NAICS code applicable to this requirement is 334516 Analytical Laboratory Instrument Manufacturing with size standard 1000 employees. BACKGROUND The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability. The National Center for Advancing Translational Sciences (NCATS) is a translational science center whose mission is to catalyze the generation of innovative methods and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions. To establish and move stem cell technologies forward tough a more centralized effort, NIH has launched the Stem Cell Translation Laboratory (SCTL) within the NCATS One of the major SCTL goals is to perform deep characterization of a large number of human pluripotent stem cell lines in order to gain better understanding of the heterogeneity within the pluripotent phenotype, as part of the Helping to End Addiction Long-term (HEAL) Initiative. This effort requires an ultrahigh toughput Sequencing system for onboard cluster generation and sequencing which provides a powerful, high toughput genomics solution and empowers users to perform studies at the toughput and sample efficiency that meets their research objectives. Purpose and Objectives This Small Business Sources Sought notice seeks to determine eligible sources capable of achieving the essential features of this potential requirement, which is to obtain one (1) High-toughput Sequencing system and accessories on a BRAND-NAME-OR-EQUAL basis to the Illumina NovaSeq 6000 and accessories manufactured by Illumina, Inc located at 5200 Illumina Way, San Diego CA 92122. The NovaSeq 6000 provides simple, streamlined production-scale sequencing of genomes, exomes, transcriptomes, and numerous other library types. The system delivers a groundbreaking combination of toughput, speed, and flexibility to complete next-generation sequencing (NGS) projects. The Xp Flow Cell Dock is required when using the NovaSeq Xp workflow that allows SCTL to load sequencing libraries into individual flow cell lanes. Project Requirements Offerors submitting a response to this Small Business Sources Sought notice shall possess the ability to achieve the essential government features specified herein. Products offered must include those salient physical, functional, or other characteristics of the NovaSeq 6000 system and accessories that are manufactured by Illumina, Inc. deemed essential in meeting the government's needs. The NovaSeq 6000 Sequencing System will serve as the end point for all of the SCTL sequencing experiments, therefore, it is imperative that this system is compatible in all aspects (form, fit, and function) with existing systems presently installed. Specifically, the system must be compatible with the existing 10x Genomics Comium Controller which is used for single-cell RNA sequencing and single-cell DNA copy number variant sequencing, as well as the existing Fluidigm C1 Single-Cell Auto-Prep System, which is a single-cell RNA sequencing system used by SCTL staff. Specifically, the following are considered essential features of this potential requirement: Multiple flow cell configurations (independently run one or two flow cells at a time, individually start or stop runs, mix and match flow cell types) enable sequencing of ~80 Gb-6000 Gb per run, providing adjustable output based on project needs. Contains streamlined workflows, automated onboard cluster generation, including automated exclusion amplification (ExAmp) chemistry to ensure consistent, reproducible run performance. RFID-encoded consumables and load-and-go reagent cartridges for user-friendly experience; reliability of matching run configurations with proper flow cell and consumable kit minimizes potential incompatibilities. Highest Output: Up to 20 billion clusters and 6000 Gb of output per 2 150 bp run (dual NovaSeq S4 flow cell run). Instrument Toughput: Generate > 3000 Gb per day using a 2 150 bp dual NovaSeq S4 flow cell run; Tunable output using multiple flow cell configurations. Output Range: ~80 Gb-6000 Gb per run depending on flow cell type, number of flow cells, and read length options. Sequencing Flexibility: Ability to run one or two flow cells at a time, choose between four flow cell types, run different flow cell types simultaneously, start and stop flow cells independently, choose from multiple read length kit configurations for each flow cell type, and run any read length up to the maximum number of cycles per kit. Sequencing methods must include: whole-genome resequencing, targeted resequencing, whole-genome de novo sequencing, sequencing of bisulfite-treated DNA, RNA sequencing, genotyping by sequencing, nucleosome positioning and comatin structure studies, ChIP-Seq, sequencing of ancient DNA samples, single-cell applications, linked long-read applications, and additional applications as developed. Products offered must be essentially equal to the specified supply described in this solicitation, which specifically includes the following unit name and Illumina manufacturer Part Number: NovaSeq Xp Flow Cell Dock; Item No.: 20021663 NovaSeq 6000 Sequencing System; Item No.: 20012850 In accordance with FAR Clause 52.217-9 OPTION TO EXTEND THE TERM OF THE CONTRACT, the following option years are applicable to this potential requirement: NovaSeq 6000 Gold Support Plan; Item No.: 20020011. CAPABILITY STATEMENTS/INFORMATION SOUGHT Interested parties are expected to review this notice to familiarize itself with the requirements of this project. Failure to do so will be at your firm's own risk. Respondents must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in this announcement; (d) quantity; (e) shipping, handling, and/or installation instructions, and/or any special delivery conditions after receipt of an order. Respondents that believe that they are manufacturers or authorized re-sellers of the equipment specified in this announcement must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in the purchase description; (d) quantity; (e) estimated price or cost; (f) shipping, handling, and/or installation charges; and delivery date after receipt of order. The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc.) pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. The information submitted must be in outline format that addresses each of the elements of this requirement. A cover page and an executive summary may be included but is not required. The response is limited to ten (10) pages. The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents' technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. Respondents must reference the subject announcement number on all correspondence related to this Small Business Sources Sought notice. The response must be submitted to Hunter Tjugum electronically, at hunter.tjugum@nih.gov, and be received prior to the closing date specified in this announcement. CONCLUDING STATEMENTS Disclaimer and Important Notes. This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s). . 75N95019Q00075 Department of Health and Human Services National Institutes of Health
1/30/19, 5:25 PM Ultrahigh Throughput Sequencing System and Dock
(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested on a Full and Open competitive basis and a written solicitation will not be issued. (ii) This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures; FAR Subpart 13.5-Test Program for Certain Commercial Items; and FAR Part 12-Acquisition of Commercial Items and is expected to exceed the simplified acquisition teshold of $250,000.00. A fixed-price type of contract is contemplated for any resulting contract entered because of this solicitation. (iii) The solicitation document and incorporated provisions and clauses are those in effect tough Federal Acquisition Regulation (FAR) Federal Acquisition Circular (FAC) Number/Effective Date: 2019-01 / January 22, 2019. (iv) The North American Industry Classification System (NAICS) code for this procurement is 334516 Analytical Laboratory Instrument Manufacturing with size standard 1,000 employees. (v) Background The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability. The National Center for Advancing Translational Sciences (NCATS) is a translational science center whose mission is to catalyze the generation of innovative methods and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions. NIH has launched the Stem Cell Translation Laboratory (SCTL) within NCATS to establish and move stem cell technologies forward tough a more centralized effort. One of the major SCTL goals is to perform deep characterization of a large number of human pluripotent stem cell lines to gain better understanding of the heterogeneity within the pluripotent phenotype, as part of the Helping to End Addiction Long-term (HEAL) Initiative. This effort requires an ultrahigh toughput Sequencing system for onboard cluster generation and sequencing which provides a powerful, high toughput genomics solution and empowers users to perform studies at the toughput and sample efficiency that meets their research objectives. Purpose and Objective: The purpose of this requirement is to obtain one ultrahigh toughput sequencing system and dock on a BRAND-NAME-OR-EQUAL basis. The brand name items are the NovaSeq 6000 sequencing system and NovaSeq XP Flow Cell Dock manufactured by Illumina, Inc located at 5200 Illumina Way, San Diego CA 92122. The NovaSeq 6000 provides simple, streamlined production-scale sequencing of genomes, exomes, transcriptomes, and numerous other library types. The system delivers a groundbreaking combination of toughput, speed, and flexibility to complete next-generation sequencing (NGS) projects. The XP Flow Cell Dock is required when using the NovaSeq XP workflow that allows SCTL to load sequencing libraries into individual flow cell lanes. Product Description: Products offered must include those salient physical, functional, or other characteristics that are deemed essential in meeting the government's needs. The NovaSeq 6000 Sequencing System or its equal will serve as the end-point for all of the SCTL sequencing experiments; therefore, it is imperative that this system is compatible in all aspects (form, fit, and function) with existing systems presently installed. Specifically, the system must be compatible with the existing 10x Genomics Comium Controller which is used for single-cell RNA sequencing and single-cell DNA copy number variant sequencing, as well as the existing Fluidigm C1 Single-Cell Auto-Prep System, which is a single-cell RNA sequencing system used by SCTL staff. In addition, the following are considered salient characteristics features of this requirement: Multiple flow cell configurations (independently run one or two flow cells at a time, individually start or stop runs, mix and match flow cell types) enable sequencing of ~80 Gb-6000 Gb per run, providing adjustable output based on project needs. Contains streamlined workflows, automated onboard cluster generation, including automated exclusion amplification (ExAmp) chemistry to ensure consistent, reproducible run performance. RFID-encoded consumables and load-and-go reagent cartridges for user-friendly experience; reliability of matching run configurations with proper flow cell and consumable kit minimizes potential incompatibilities. Highest Output: Up to 20 billion clusters and 6000 Gb of output per 2 150 bp run (dual NovaSeq S4 flow cell run). Instrument Toughput: Generate > 3000 Gb per day using a 2 150 bp dual NovaSeq S4 flow cell run; Tunable output using multiple flow cell configurations. Output Range: ~80 Gb-6000 Gb per run depending on flow cell type, number of flow cells, and read length options. Sequencing Flexibility: Ability to run one or two flow cells at a time, choose between four flow cell types, run different flow cell types simultaneously, start and stop flow cells independently, choose from multiple read length kit configurations for each flow cell type, and run any read length up to the maximum number of cycles per kit. Sequencing methods must include: whole-genome resequencing, targeted resequencing, whole-genome de novo sequencing, sequencing of bisulfite-treated DNA, RNA sequencing, genotyping by sequencing, nucleosome positioning and comatin structure studies, ChIP-Seq, sequencing of ancient DNA samples, single-cell applications, linked long-read applications, and additional applications as developed. One-year service plan. Products offered must be essentially equal to the specified supply described in this solicitation, which specifically includes the following unit name and Illumina manufacturer Part Number: NovaSeq Xp Flow Cell Dock; Item No.: 20021663 NovaSeq 6000 Sequencing System; Item No.: 20012850 In accordance with FAR Clause 52.217-9 OPTION TO EXTEND THE TERM OF THE CONTRACT, the following option years are applicable to this requirement as two (2) additional one-year optional periods of performance: NovaSeq 6000 Gold Support Plan; Item No.: 20020011: Quantity two (2) Shipping and handling cost must be included in any quotation submitted in response to this solicitation. Offerors must indicate if any applicable extended warranty coverage is only offered at time of purchase in accordance with the Offeror's standard commercial practice, or if warranty may be tendered on an optional year basis, priced on a per annum basis, for a total contract warranty period term not to exceed five (5) years. If Offeror's standard commercial warranty is less than five years, offeror shall also include pricing for extended warranty coverage/preventive maintenance, for a total period of up to five (5) years. Delivery, Inspection and Acceptance: Offerors should indicate an estimated delivery time in full after receipt of an order and any other training or factory warranty schedules in accordance with standard commercial practice, or as applicable. Inspection and acceptance will be performed at a Government facility located at 9800 Medical Center Drive, Rockville, MD 20850. The Government expected build time and delivery is 90 days after contractor receipt of any contract resulting from this solicitation. (vi) The provision at FAR clause 52.212-1, Instructions to Offerors - Commercial Items, applies to this acquisition. The following is provided as an addendum to FAR clause 52.212-1, Instructions to Offerors: Installation and any onsite training should be quoted on separate line items. Shipping and handling cost must be included in any quotation submitted in response to this solicitation. Offerors should indicate an estimated delivery time in full after receipt of an order and any other training or warranty schedules applicable. Offerors must indicate if any applicable extended warranty coverage (EWC) is only offered at time of purchase in accordance with the Offeror's standard commercial practice, and the length of time of such warranty. SMALL BUSINESS SUB CONTRACTING PLAN: The HHS SUBCONTRACTING PLAN TEMPLATE is attached to this solicitation. Pursuant to NIH's priority of Small Business participation, Offerors shall be required to submit a subcontracting plan in accordance with the terms of the clause entitled "Small Business Subcontracting Plan," at FAR Clause No. 52.219-9 prior to contract award. (vii) The provision at FAR clause 52.212-2, Evaluation - Commercial Items, applies to this acquisition. The following is an addendum to 52.212-2. Responses received to this RFQ will be evaluated on the basis of Lowest Price Technically Acceptable (LPTA). Offeror's submitting a response must provide a completed Price Quote to be evaluated and considered for award. Price quotes must indicate fixed unit pricing, shipping cost, total cost, estimated delivery date, and warranty and training considerations. Responses will be evaluated based on contractor's ability to meet the government's SALIENT CHARACTERISTICS stated in this notice and on ability to deliver required supplies. Responses to this solicitation must include clear and convincing evidence of the Offeror's capability of fulfilling the requirement as it relates to the essential government requirements stated in this solicitation. Under the lowest-price technically acceptable (LPTA) process, Offerors' will be determined to be "technically acceptable" or "technically unacceptable" based on an evaluation of responses against the following factors to determine if, as submitted, the proposal is technically acceptable. (1) Technical Capability: Offerors shall clearly indicate possessing the technical capability to achieve the essential salient characteristic features of this requirement. The Offeror must indicate in its quotation the ability to provide all of the requirements. The Offeror must indicate in its quotation the ability to meet the delivery requirements. Offerors submitting a response with documented technical specifications and evidence of possessing the technical knowledge, qualifications and capability and management structure needed in successfully achieving this requirement will be determined to be technically acceptable. Respondents that believe that they are manufacturers or authorized resellers of any BRAND-NAME product specified in this announcement must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in the purchase description; quantity; estimated price or cost; shipping, handling, and/or installation charges; and delivery date after receipt of order. (viii) The provision at FAR Clause 52.212-3, Offeror Representations and Certifications Commercial Items, applies to this acquisition. (ix) The provision at FAR Clause 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. An addendum to FAR Clause 52.212-4, Contract Terms and Conditions-Commercial Items, Offeror Representations and Certifications Commercial Items, applies to this acquisition. The addendum includes the following FAR Clauses, provisions, terms and conditions: (a) 52.211-6 Brand Name or Equal (Aug 1999); (b) 52.217-9 Option to Extend the Term of the Contract (Mar 2000). (x) The provision of FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders Commercial Items applies to this acquisition and the clause at 52.22251, Exemption from Application of the Service Contract Act to Contracts for Maintenance, Calibration, or Repair of Certain EquipmentRequirements (Nov 2007) (41 U.S.C. 351, et seq.) applies to this acquisition. See attached for the appendix to 52.212-5. In addition, the following FAR provisions are applicable to this solicitation and incorporated by reference: 52.204-7 System for Award Management (Oct 2018), 52.204-13 System for Award Management Maintenance (Oct 2018), 52.204-16 Commercial and Government Entity Code Reporting (Jul 2016), 52.204-18 Commercial and Government Entity Code Maintenance (Jul 2016). (xi) There are no additional contract requirement(s) or terms and conditions applicable to this acquisition. (xii) The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. (xiii) Responses to this solicitation must include sufficient information to establish the interested parties' bona-fide capabilities of providing the product or service. The PRICE QUOTE shall include: unit price, list price, shipping and handling costs, delivery days after contract award, delivery terms, prompt payment discount terms, F.O.B. Point (Destination or Origin), product or catalog number(s); product description; and any other information or factors that may be considered in the award decision. Such factors may include: past performance; special features required for effective program performance; trade-in considerations; probable life of the item selected as compared with that of a comparable item; warranty considerations; maintenance availability; and environmental and energy efficiency considerations. Responses to this solicitation must include clear and convincing evidence of the offeror's capability of fulfilling the requirement as it relates to the technical evaluation criteria and a price proposal. Offerors must include a completed copy of the provision at 52.212-3, Offeror Representations and Certifications Commercial Items, with their offer, or provide a copy of the valid certifications registrations and Representation and Certifications from the System of Award Management (SAM) applications at www.SAM.gov, which may be indicated on the Offeror's cover sheet. In addition, the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All Offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." All responses must be received no later than the date specified in this announcement. Late responses will not be accepted. All responses must reference the subject solicitation number. Responses must be submitted electronically to hunter.tjugum@nih.gov. Facsimile responses will not be accepted. For information regarding this solicitation, contact Hunter Tjugum by email at hunter.tjugum@nih.gov or by phone at 301-827-5304.. 75N95019Q00075 Department of Health and Human Services National Institutes of Health
3/26/19, 3:59 PM