Procurement of All-In-One Fluorescence Microscope

PRE-SOLICITATION NOTICE OF INTENT
NON-COMPETITIVE

Solicitation: HHS-NIH-NIDA-SSSA-NOI-17-764

Title: Procurement of All-In-One Fluorescence Microscope

INTRODUCTION
PURSUANT TO FAR Subpart 5.2-Synopses of Proposed Contract Actions, THIS IS A PRE-SOLICITATION NOTICE OF A PROPOSED CONTRACT TO ACTION.

THIS IS A PRE-SOLICITATION NON-COMPETITIVE NOTICE OF INTENT TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME).

The National Institute on Drug Abuse (NIDA), Station Support Contracts and Simplified Acquisitions Branch (SS/SA) on behalf of the National Institute on Aging (NIA) intends to negotiate and award a contract for the All-In-One Fluorescence Microscope with Keyence Corporation of America, 669 River Drive, Suite 403, Elmwood Park, NJ 07407.

NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE
The intended procurement is classified under NAICS code 333314 - Optical Instrument and Lens Manufacturing with a Size Standard of 500 Employees.

REGULATORY AUTHORITY
The resultant contract will include all applicable provisions and clauses of the Federal acquisition Requlation (FAR) in effect through the Federal Acquisition Circular (FAC) 05-95, January 19, 2017.

STATUTORY AUTHORITY

This acquisition is conducted as non-competitive under the authority of 41 U.S.C. 253(c) under provisions of the statutory authority of FAR Subpart 6.302-1 - Only one response source and no other supplies or services will satisfy agency requirements.

DESCRIPTION OF REQUIREMENT

Project Description
The All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of transcription factors in cell differentiation and aging rates of human iPS cells. The instrument will allow live cell imaging as well.

The potential NIA applications of this microscope include, but are not limited to:
• Ongoing studies of senescence (Gorospe lab, LGG) and aging in mice (SLAM, de Cabo lab, TGB) which can be facilitated by the user-friendly, high throughput, semi-automated imaging of tissue microarrays.
• Single cell DNA damage assays (Comet assays) can be more efficiently imaged (Beerman lab, TGB).
• Mitochondrial volume reconstructions can be rendered for whole organisms, tissue culture or isolated mitochondria, complementing the use of Seahorse system for measuring mitochondrial ROS and ATP generation.(Tyler Demarest, Bohr lab, LMG). For example, cultures analyzed in Seahorse plates can be re-imaged with the Keyence microscope system.
• Wound invasion assays of NF-kB reporter expressing fibroblasts can be assessed on this setup as well. (Erik Martin, Sung lab, LMBI)

Purpose and Objectives
The purpose of this acquisition is to procure a small stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multi-well plates, coverslips and slides. The microscope must have bright field, fluorescence, and phase-contrast imaging with the capability for image stitching, Z-stack image capturing, and coordinate-specific condition setting.

Delivery Schedule
Within 30 days week after receipt of order (ARO).

CLOSING STATEMENT
This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice.

Responses to this solicitation must include clear and convincing evidence of the offeror's capability of fulfilling the requirement as it relates to the technical evaluation criteria. The price proposal must include the labor categories, an estimate of the number of hours required for each labor category, fully loaded fixed hourly rate or each labor category, breakdown and rationale for other direct costs or materials, and the total amount.

 

Salient characteristics: See attached Purchase Description for more details.

Quantity: One (1)

In addition the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov."
A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement.

All responses must be received by Monday, September 25, 2017 at 10AM, Eastern Standard Time, and reference number HHS-NIH-NIDA-SSSA-NOI-17-764. Responses may be submitted electronically to Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov.

Fax responses will not be accepted.

Extensions to the deadline will not be granted.

Please contact Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov or (301) 594-1928 with any questions.

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:
The purpose of this acquisition is to procure a small stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multi-well plates, coverslips and slides. The microscope must have bright field, fluorescence, and phase-contrast imaging with the capability for image stitching, Z-stack image capturing, and coordinate-specific condition setting.

2. Background Information and Objective: The All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of transcription factors in cell differentiation and aging rates of human iPS cells. The instrument will allow live cell imaging as well.

The potential NIA applications of this microscope include, but are not limited to:
• Ongoing studies of senescence (Gorospe lab, LGG) and aging in mice (SLAM, de Cabo lab, TGB) which can be facilitated by the user-friendly, high throughput, semi-automated imaging of tissue microarrays.
• Single cell DNA damage assays (Comet assays) can be more efficiently imaged (Beerman lab, TGB).
• Mitochondrial volume reconstructions can be rendered for whole organisms, tissue culture or isolated mitochondria, complementing the use of Seahorse system for measuring mitochondrial ROS and ATP generation.(Tyler Demarest, Bohr lab, LMG). For example, cultures analyzed in Seahorse plates can be re-imaged with the Keyence microscope system.
• Wound invasion assays of NF-kB reporter expressing fibroblasts can be assessed on this setup as well. (Erik Martin, Sung lab, LMBI)

3. Generic Name of Product: All-In-One fluorescence microscope

4. Purchase Description:
1 BZ-X710 BZX Fluorescence Microscope
2 BZ-X700E BZX Fluorescence Microscope
3 972318 Win10 PC for BZ-X Dell T3420
4 972072B BZX Accessory 23'' Monitor
5 BZ TRAINING BZ Training
6 BZ-H3XD BZX Software
7 BZ-H3XT BZX Software BZX Time Lapse
8 BZ-H3AE BZX Software BZX Analyzer Software
9 OP-87762 BZX Accessory BZX DAPI Filter
10 OP-87763 BZX Accessory BZX GFP Filter
11 OP-87765 BZX Accessory BZX Texas Red Filter
12 OP-87766 BZX Accessory BZX Cy5 Filter
13 972081 CFI PLAN FLUOR DL 4X LENS
14 971936 CFI PLAN FLUOR DL 10X LENS
15 971962 Nikon Lens (20x ELWD Phase) for BZ S PI FI ELWD ADM 20xC
16 971963 Nikon Lens (40x ELWD Phase) for BZ S PL FL ELWD ADM 40xC
17 972082 INUG2-KIW STAGE TOP INCUBATOR

 

5. Salient characteristics:
Microscope unit Basic optical system Inverted fluorescence phase contrast microscope
Objective lenses Nikon CFI 60 Series infinite optical system
Observation modes Bright field, Fluorescence (wide-field/sectioning), Phase contrast (PhL, Ph1, Ph2), Oblique illumination
Objective lens switching Six-mount electronic revolver
Image-formation optical system Fixed image-forming lens, electronic LC filter insertion / removal unit
Electronic XY stage 100×64 mm stroke, 1 um pitch min.
Electronic Z stage 8 mm stroke, 0.1 um pitch min.
Electronic filter turret Up to four filters can be mounted. Automatic position recognition and automatic excitation shutdown during filter replacement
Fluorescent incident illumination Optical sectioning system
Fluorescence dimming mechanism Electronic dimming (0.3% 5% 10% 20% 40% 100%)
Transmitted illumination optical system Operating distance : 30 mm, Pop-up mechanism (with automatic lamp off function)
Transmitted illumination mechanism Electronic switching, Electronic bright-field aperture (0% 20% 40% 60% 80% 100%) / Phase-contrast slit (Ph1 / Ph2)
Transmitted light source LED 3.7W , 50,000 hours (average)
Fluorescent incident light source Metal halide lamp 80W , 2,000 hours (average)
Specimen enclosure The stage is fully contained in a built-in darkroom.
Camera unit Image pick-up element 2 / 3 inch, 2.83 million pixel monochrome CCD (colourised with LC filter)
CCD cooling mechanism Peltier cooling : 5°C 41°F (at ambient temperature of -25°C -13°F)
Output signal, gradations Monochrome 14bit / 8bit , 8 bit for RGB each
Frame rate 15 fps for monochrome recording (up to 95 fps with binning), 8.5 fps for color recording
Binning On-chip binning (2×2、3×3、4×4、8×8、12×12)
Number of pixels in recorded image 4,080 × 3,060 max (12.5 million pixels, high-quality interpolation)
Video capture 8-bit monochrome: 15 fps for 1,280 x 960
With binning: 28 fps for 960 x 720, 40 fps for 640 x 480, 50 fps for 480 x 360, 75 fps for 240 x 180, 95 fps for 160 x 120
Color: 8.5 fps for 1,280 x 960
Electronic shutter Auto : 1 / 7500 to 60 sec. (77 increments)
Auto Exposure method Arbitrary area, average / peak
Gain 0dB、+6dB、+12dB、+18dB
White balance MANUAL, PUSH-SET
Black balance
Observation software Multi-color image capturing, Auto focus, Quick full-focusing, Scale display, Electronic revolver control, Electronic stage control
Controller Applicable OS Windows7 Professional 64bit SP1*1
PC interface USB2.0
Ambient temperature +15 to +35°C 59 to 95°F
Relative humidity 35 to 80% RH (No condensation)
Dimensions Head : 517 (H) × 340 (W) × 496 (D) mm*2 / Controller : 227.5 (H) × 125 (W) × 403 (D) mm
Weight Head : 33 kg / Controller : 4.8 kg
Power voltage 100 to 240 VAC ±10% 50 / 60 Hz
Power consumption 250VA
Overvoltage category II
Pollution degree 2
Software (optional) Sectioning Module Optical sectioning image mode
Advanced Observation Module Type Navigation, Image stitching, Z-stack image capturing, Coordinate-specific condition setting
Navigation Module Navigation
Image stitching Module Image stitching, Z-stack image capturing, Coordinate-specific condition setting
Time-lapse Module Time-lapse imaging, Video capturing
Advanced Analysis Software Image stitching, Haze reduction, Full focus
Measurement Module XY measurement, Area measurement, Brightness measurement (line profile, histogram)
3D Module Real-time 3D analysis, XYZ slicing, Maximum projection
Hybrid Cell Count Module Cell count (phase contrast, bright field, fluorescence images), Mask cell count
Macro Cell Count Module Macro Cell Count (batch analysis of multiple images)

6. Quantity: One (1)

7. Delivery Date: Within 30 Days after receipt of order (ARO)