Leica TCS SP8 AOBS Filter Spectral Confocal System

Leica TCS SP8 AOBS FilterFree
Spectral Confocal
System including:
DMi8 CS Automated Inverted Microscope
Trinocular
Motorized Focus with AFC
Spill protection for DMi8
Optical Outfit EL6000 + motorized ICT
Transmitted Light Brightfield Detector
XY Scanning stage inverted (LMT200 Upgrade)
Scan optics module HIVIS no rotation
Tandem scanner 8 kHz
X1 Port Emission Access
X1 Port adaptation for fibers (APD) with APD
detection Unit
Three Internal Detector Channels (PMT)
Two Internal Detector Channels (HyD)
Laser 405 nm AOTF Flexible
Laser Kit ROYBV for AOBS
SPR for 690nm Laser
Premium Workstation, Monitor
This configuration does not include an antivibration
table or compressor. Customer agrees
to provide a sufficient vibration isolation table
that is properly floated at time of installation
The focus system « Adaptive Focus Control »
(AFC) maintains a userspecified
focus plane over
time. This dramatically improves the stability of
longterm experiments. Confocal module with patented filterfree
spectral
Leica SP detector for up to five individually
regulatable channels. Highly efficient spectral
separation by unique prism design. Equal brightness between channels due to Wshaped slit design. Leica patented AcoustoOptical
Beamsplitter (AOBS) provides optimal spectral
flexibility with up to 8 laser lines simultaneously.
Highly synergistic in combination with prism and
Leica SP detector. Unique selectivity for optimal
image contrast, undistorted recording of emission
spectra for ideal dye separation and exquisit signal
efficiency resulting in maximal sample viability.

Scan optics with HIVIS coating for maximal
transmission from 400800 nm.

True Confocal Scanning system (TCS) with unique
X2Y design warrants planar, parallaxfree
illumination enabling the largest field of view.
Switchable between resonant and nonresonant
mode for optimal scanning speeds. Using
resonant scanning a higher fluorescence quantum
yield and reduced photobleaching are achieved.
The continuosly adjustable speed of the nonresonant
scanner (1 1800 Hz) is ready for single
molecule imaging, image correlation and imaging
of rapid dynamics alike (up to 72 frames / second
at 512 x 32 scan format). The maximal image
resolution of 64 Megapixels allows sampling
optimally, also with low magnification lenses.

Leica HyD detector povides supersensitive
photon detection making it ideal for low light and live cell
imaging. Thanks to its very low dark noise it
produces brilliant imagery rich in contrast and
fidelity for finest details. The simple geometry of
the GaAsP photocathode and the hybrid design
minimize losses within the detector setting it apart
from any PMT, including GaAsP PMTs, in terms of
photon detection efficiency. The new Leica HyD
provides a timegating option for optimal contrast
in conjunction with the optional WLL and improved
superresolution with gated STED (optional).

Completely redesigned UV / 405 nm light path
offers highly stable 405 and 355 nm laser
incoupling.

Premium HP workstation with high performance
GPU for fast handling of large data sets. High
brilliance 30” LCD screen for the best presentation
of image data.

LAS AF 3 imaging software provides intuitive
control over the hardware of the SP8AOBS. Image
acquisition, processing and quantification are all offered in one extendable package.

All responsible sources may submit a response that will be considered. The Government reserves the right to make a decision whether this requirement is available for competition. All responses shall be submitted no later than 2:00 PM eastern standard time August 14, 2016 to Lisa Hinton 5601 Fishers Lane, Suite 3A68, Rockville, MD 20852 email, hintonl@mail.nih.gov.