Salient_Characteristics__Requirement_of_Equipment.pdf

SALIENT CHARACTERISTICS

1. Characteristics:

A. ≤ 20” x 25” dimensional footprint B. Complete system that includes all components for operation.

C. The system must be completely alignment free. Laser and optical alignments must be fixed alignments (no stepper motors) and require no user alignment.

D. The cell sorter must have gel coupled quartz cuvette where the laser beams interrogates the cells.

E. The cell sorter must have a removable nozzle which can be sonicated. This nozzle must be keyed and require no optical/laser system alignment when replaced.

F. The cell sorter must automate system startup including de-bubbling the sheath fluid, automate optimization of the drop-drive frequency, sheath pressure and amplitude.

G. The cell sorter must Blue 488nm laser and Red 640nm laser with 8 parameters FSC, SSC and 6 colors. System must be upgradeable to add a 3rd laser with 3 additional PMT’s for a total 11 parameters FSC, SSC and 9 colors.

H. The cell sorter must have spatially separated beams each with its own unique optical channel.

I. The cell sorter optical filters must have an electronic identifier chip. This identifier chip communicates with computer updating system configuration after a filter has been changed.

J. The cell sorter must have temperature control of the sample and the sort tube holder.

K. The cell sorter must have the ability to sort into a temperature controlled holder which supports

6-, 24-, 48-, 96- and 384-well plates.

L. The cell sorter must have guaranteed on-site emergency service within 48 hours of initial request.

M. The ability to gate and sort two or more populations in real-time and simultaneously.

N. Must be high-throughput, capable of acquiring 40,000 and sorting 10,000 events per second.

O. Needs High-sensitivity and resolution rated at a minimum of 80 MESF (Molecules of

Equivalent Soluble Fluorochrome) for FITC and 30 MESF for PE. This enables the discriminating and detecting of cells with low fluorescence, rare events, small particles and cellular DNA.

P. The system must be capable of saving the data in FCS file industry-standard format.