SOW(RNAseq)_Raj Majumdar.docx

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RNA analysis of sugar beet samples
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001205405000658_RMajumdar
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Statement of Work United States Department of Agriculture (USDA) Agricultural Research Service (ARS), Northwest Irrigation and Soils Research Laboratory (NWISRL) For RNA analyses of sugar beet samples

1. Objective

The United States Department of Agriculture (USDA), Agricultural Research Service (ARS) requires analysis of RNAs originating from sugar beet samples infected with pathogens, for results requested per each sample batch. There will be a total of 115 sugar beet samples to be sent in the fall.

2. Scope The purpose is to acquire the required analysis of sugar beet samples infected with virus/fungus/bacteria, for results requested per each sample batch. There will be a total of 115 total samples amongst which, 63 samples are related to small RNA sequencing (sRNAseq) and 52 samples for long (regular transcripts) RNA sequencing (RNAseq). We anticipate a turnaround time of 4-6 weeks. We will submit samples for Task 1 and Task 2 separately.

Task 1 - Analysis of 63 sugar beet samples (curly top infected) submitted in Fall of 2020

TEST

sRNAseq library preparation sRNAseq library quality control sRNA library sequencing and generation of sequencing data sRNA sequencing data analysis

Task 2 - Analysis of 52 sugar beet samples (Rhizoctonia and Leuconostoc infected) submitted in Fall of 2020

TEST

RNAseq (regular transcripts) library preparation

RNAseq library quality control

RNA library sequencing and generation of sequencing data

RNA sequencing data analysis

3. Deliverables We anticipate a turnaround time of 4-6 weeks. We will require a report at the end of that time, and an invoice for the completed work.

4. Government Furnished Resources None

5. Administrative Considerations None

6. Period(s) and Place of Performance

Period of Performance Tasks 1-2 – Sept 22nd, 2020 through Nov 30th, 2020

Place of Performance – USDA, ARS, NWISRL in Kimberly, ID

7. Security Requirements Disclosure of USDA information. USDA must approve any press releases or interviews with industry prior to action.

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