Statement_of_Work.pdf

STATEMENT OF WORK

TITLE: GC-MS System

I. BACKGROUND INFORMATION

The National Institute of Standards and Technology (NIST), Material Measurement Laboratory

(MML), Biomolecular Measurement Division (BMD) in Gaithersburg, Maryland is responsible for the development of chemical databases to accomplish NIST’s mission to carry out collection, compilation, critical evaluation, publication and sale of standard reference data in accordance with

Public Law 90-396. 90th Congress, H.R. 6279, July 11, 1968. The chemical databases provide standard reference data for laboratories, both within NIST and to the worldwide scientific community. The Mass Spectrometry Data Center (MSDC), a component of the BMD, is responsible for the development of the NIST/NIH/EPA Mass Spectral Library and special Mass

Spectral Libraries (hereinafter, the Libraries) (http://www.nist.gov/srd/nist1a.cfm, http://chemdata.nist.gov/dokuwiki/doku.php?id=peptidew:start). This development includes generating and analyzing data obtained by Gas Chromatography-Mass Spectrometry (GC-MS).

II. OBJECTIVE

The objective of the project is to facilitate the expansion of the NIST/NIH/EPA Mass Spectral

Library by adding a GC-MS System to the MSDC’s laboratories.

III. SCOPE

The contractor shall provide a GC-MS system meeting the specifications listed below. The

Contractor shall install the instrument, provide training, and provide maintenance.

IV. SYSTEM REQUIREMENTS

The Contractor shall provide one Agilent brand name GC-MS System, 5977B EI/CI MSD

(G7078BA) / 7890B Series GC (G3440B), or equal. The Contractor shall also provide one

GERSTEL brand name autosampler, MultiPurpose Sampler Robotic for Liquid

Injection (GER 100025-000-02), or equal. The salient characteristics are as follows:

A. Mass Spectrometer

1. Mass analyzer: Single quadrupole

2. Electron ionization (EI) mode and chemical ionization (CI) mode

3. Sensitivity: S/N 1500:1 (EI) with 1 pg Octafluoronaphthalene (OFN), scanning 50-300 u at 272 u ion.

4. Instrument Detection Limit: 10 fg (EI).

5. Scan speed: The scan speed of the instrument must be up to 12,500 u/sec.

6. Mass range: The instrument must scan from 2 u – to 1000 u.

7. Ion source temperature: Ion source temperature must have the capability to heat up to 350°C.

8. The heated quadrupole: The quadrupole must able to be heated up to 200°C.

9. Scan increment (step size): Scanning at the increments of 0.1 u.

10. Tune: The Mass Spectrometer must have standard and optional spectra tuning capabilities.

“Standard tune” means a standard response over the entire scan range, and “optional tune” can discriminate low masses and show high mass peaks with greater intensities.

11. Oil free foreline pump.

B. Gas Chromatograph

1. The Gas Chromatograph must offer the capability to install two inlet ports.

2. Inlet sealing system must allow for quick easy liner changes without using tools.

3. The inlet vent trap line must be replaceable for the effective cleaning.

4. Oven dimensions must be not less than: 27 x 30 x 15 cm and not more than 35 x 35 x 25 cm.

5. Oven operating temperature range (Ambient+5 °C to 450°C).

6. Oven temperature set-point resolution shall be 0.1 °C.

7. Split/splitless capillary inlet shall have the split ratio up to 7,500:1, and maximum temperature 400°C capability.

C. Autosampler

1. XYZ robotic sample handling with syringe concept.

2. Mounted on the GC-MS System.

3. Injection on either GC inlet or both.

4. Capable of sample preparation during GC analysis of the preceding sample.

5. Must include a solvent wash station.

6. Capable of vial capacity of up to 648 x 2 mL vials.

7. Must include compatible 54 x 2-mL trays.

8. Liquid injection techniques including standard and sandwich using air or solvent.

9. Universal syringe holder capable of holding 1 µL – 1000 µL syringes. Injection volume range of .1 µL – 1000 µL.

10.

11. Software controlling the autosampler must be compatible with, and integrated with, the

Agilent ChemStation Data Analysis software controlling the GC-MS System.

D. Computer System and Software

The instrument must include a computer system and LaserJet printer. The instrument must include a software package that controls the instrument, controls data acquisition, includes data mining tools, and includes processing tools. The software package must also include a post-acquisition software tool, compatible with the data acquisition and processing software, that aids in compound identification. The software package must calibrate the mass position of the peak and calibrate the MS line shape to increase mass accuracy and spectral accuracy to aid in compound identification.

E. Safety Features

1. The mass spectrometer shall have an automatic interrupt system. In addition, analyzer temperature zone and operating voltages shall be interlocked with vacuum pump speed to prevent instrument damage due to operation at excess pressure.

2. The Gas Chromatograph must have: (a) a hydrogen safety shutdown system that monitors the hydrogen flow and shuts down the gas flows and heaters if a leak is detected, and (b) an oven explosion protection built into the oven door. The Mass Spectrometer analyzer shall not fragmentize in the event of a hydrogen deflagration.

3. The Gas Chromatograph and Mass Spectrometer must have a United States safety certification by an accredited agency.

V. DELIVERY

The instrument shall be delivered within three months from the date of the award of this requirement in Building 221 at the National Institute of Standards and Technology (NIST), 100

Bureau Drive, Gaithersburg, MD campus. FOB destination.

VI. INSTALLATION

The Contractor shall install the instrument in Building 221 at NIST Gaithersburg, MD.

Installation shall include, at a minimum, uncrating/unpacking of all equipment, set-up and hook-up of the instrument, start-up, demonstration of specifications, and removal of all trash. The

Contractor shall demonstrate that the instrument is running in accordance with the System

Requirements above. The contractor shall provide samples for on-site demonstration of the performance of the GC-MS instrument for the specifications listed above. Installation shall take place between 8:30 am and 5:00 pm Eastern Time, Monday through Friday, except Federal

Holidays (hereinafter “normal duty hours”), and shall be coordinated with the NIST Technical

Point of Contact (TPOC). Installation shall be completed no later than two weeks from the date of delivery.

VII. TRAINING

The contractor shall conduct training at NIST, Gaithersburg, MD, for up to ten NIST personnel covering normal operation, system/solution functions, data administration, troubleshooting, and routine maintenance. Training shall be provided during normal duty hours, and shall be coordinated with the NIST TPOC to ensure maximum availability of NIST personnel. The training shall be completed no later than 30 days after installation.

VIII. WARRANTY AND SERVICE PLAN

The contractor shall warrant the instrument for a period of one year, to commence after delivery, installation, training, and acceptance of the instrument by the government. The warranty shall include unlimited repair visits in the event to of instrument failure during normal duty hours. The contractor shall cover expenses required for repairs, including parts, labor, travel, and per diem.

The contractor shall also provide a four-year service plan, to commence after the expiration of the one-year warranty. The contractor shall provide maintenance and repairs under the service plan, as follows:

1. The contractor shall respond to an unlimited number of requests for service calls in the event of instrument failure. The contractor shall respond to service calls during normal duty hours.

2. The contractor shall cover all expenses for the maintenance and repair of the instrument, including all parts, labor, travel, and per diem.

3. The contractor shall provide all consumables and supplies required for repair, including normal wear-and-tear items such as liners, seals, lamps and tubing when needed.

4. The contractor shall repair/replace all parts required for the operation of the instrument.

5. All repairs must result in the instrument meeting manufacturer’s specifications and the system requirements stated above.

6. The contractor shall provide maintenance and repair by factory-trained technicians using parts, supplies, and consumables authorized for use by the manufacturer.

IX. PLACE OF PERFORMANCE

Installation, training, and warranty/maintenance services shall be performed at the National

Institute of Standards and Technology (NIST), 100 Bureau Drive, Gaithersburg, MD campus, during normal duty hours.

X. GOVERNMENT FURNISHED PROPERTY

The government will not furnish any property to the Contractor in fulfillment of this requirement.

XI. GENERAL INFORMATION

Security: NIST is a restricted campus. An identification badge is required for access for entry into buildings and also is shown to the armed Security Police when entering the campus. The

Government will submit the name of a contractor employee to the NIST Office of Security in order for a contractor employee to enter the NIST campus to perform on-site preventive maintenance or repairs of the instrument.

Identification Badges: Contractor employees shall comply with NIST identification and access requirements. The Contractor employee is responsible for absences due to expired identification and access documents. Each Contractor employee shall wear a visible identification badge provided by the NIST Security Office. The badge must show the full name, title, and if required by NIST, the words “Contractor” in front. The Contractor employee shall turn in the NIST identification badge and vehicle pass to the TPOC, or Contracting Officer (CO) upon termination of their services under this contract.

Vehicle Registration: All Contractor employees must register their vehicles with the NIST

Security Office to gain access to the campus. A valid driver’s license, Government-furnished civilian ID, proof of insurance and current registration must be presented to the NIST Security

Office, at which time a NIST vehicle pass will be issued. The pass shall be displayed on the vehicle’s rear view mirror in accordance with instructions. The Contractor employee shall follow NIST procedures for removal and turn-in of the vehicle pass upon termination of services under this contract.