REDACTED JUSTIFICATION AND APPROVAL.pdf

Project description. The FACSymphony S6 Cell Sorter is a versatile 48 parameter flow cytometer equipped with 5 fixed-aligned, air-cooled lasers.

Additionally, the FACSymphony S6 can simultaneously sort 6 unique cell populations into assorted collection vessels including numerous tubes and multi-well plates. The FACSymphony S6 has an intuitive user interface (BD FACSDiVa software) that enables on screen instructions and automated tasks allowing easy operation of the instrument. This instrument can easily handle complex sorting studies such as cells expressing fluorescent proteins (GFP, dTomato, mCherry), phenotypic markers, and/or DNA content at the single cell level. The FACSymphony S6 Cell Sorter is compatible with our current instruments allowing for panels and sorting protocols to be easily transferred to this instrument.

Finally, the system has an independent aerosol management (AMS) system to provide both personnel and product protection.

Requirement type.

1. Research & development (R&D)

2. R & D support services

3. Support services (non-R&D)

4. X Supplies/equipment

5. Information technology (IT)

6. Construction

7. Architect-engineer (A & E) services

8. Design-build

Other (specify): ____________________

Type of action.

X New requirement Follow-on Other (specify): ______________________

Proposed contract/order type.

X Firm-fixed-price Other fixed-price (specify, e.g., fixed-price award-fee, fixed-price incentive-fee): __________ Cost-plus-fixed-fee Other cost reimbursement (specify, e.g., cost-plus-award-fee, cost-plus-incentive-fee): ___________ Time and materials Indefinite delivery (specify whether indefinite quantity, definite quantity, or requirements): ___________ Other (specify): ___________ Completion Form Term form

Acquisition identification number. 75N96023Q00046 Intent to Sole Source Synopsis posted on 5/22/2023 via SAM.gov.

Total estimated dollar value and performance/delivery period. The estimated dollar value is

. We are requesting an award on or before 9/30/23. The anticipated delivery for this item is estimated at 6 months a this is a long lead item.

4. Identification of the statutory authority permitting other than full and open competition.

X This acquisition is conducted under the authority of 41 U.S.C. 3304(a)(1) and FAR Subpart 6.302-1, only one responsible source and no other supplies or services will satisfy agency requirements. Specifically, competition is limited to an item peculiar to one manufacturer (brand-name justification), in accordance with FAR Subpart 11.105. In addition, the procedures in FAR Subpart 13.5, Simplified Procedures for Certain Commercial Products and Commercial Services, are being used in accordance with 41 U.S.C. 1901, Simplified Acquisition Procedures.

This acquisition is conducted under the authority of section 4202 of the Clinger-Cohen Act of 1996.

This acquisition is conducted under the authority of the Services Acquisition Reform Act of 2003 (41 U.S.C. 428a).

5. Demonstration that the proposed contractor(s) unique qualifications or the nature of the acquisition requires use of the authority cited.

a. Name and address of the proposed contractor(s).

Becton Dickinson & Co BD Biosciences Business Unit One Becton Drive Franklin Lakes, NJ 07417 Tel: (855) 236-2772 Fax: (408) 954-2736

b. Nature of the acquisition and proposed unique qualifications of the contractor(s).

The Flow Cytometry Center (FCC) requests the purchase of a BD FACSymphony S6 Cell Sorter to replace our current 13-year-old FACSAriaII flow cytometer located in the Flow Cytometry Center at NIEHS. As our current FACSAriall serves as both a cell sorter and a cell analyzer, it is critical that the requested instrument will also need to function in both capacities. The requested cell sorter needs to have at a minimum 48 versatile parameters equipped with at least 5 fixed-aligned, air-cooled lasers, including a 355 nm, 405 nm, 488 nm, 561 nm, and 637 nm laser lines. Additionally, the instrument needs to have the capability of detecting 10 parameters from the 355 laser, 14 parameters from the 405 nm laser, 9 parameters from the 488 nm

10. Listing of sources, if any, that expressed an interest, in writing, in the acquisition.

No other sources have expressed an interest, in writing, in the proposed acquisition.

11. Statement of the actions, if any, the agency may take to remove or overcome any barriers to competition before any subsequent acquisition for the required supplies or services.

Prior to any subsequent acquisition for the required item, we will continue conduct Market Research, in accordance with FAR Part 10, to determine if any other vendor's products come to the market that are capable of meeting the Government's requirements.