Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Institute of Aging (NIA) requires the acquisition supplies and reagents for the Center for Alzheimer’s Disease and Related Dementias disease research for gene expression analysis. The items required are of Single Cell Gen Expression reagents and consumables for rapid single cell RNA-seq library generation.

2. Background Information and Objective: imaging technology improves to capture NIA is creating a national resource of isogenic sets of Alzheimer’s Disease and Related Dementias mutation iPSC lines, complimented by foundational phenotypic datasets from differentiated disease-relevant cells derived from these lines. It is anticipated that this resource will substantially accelerate the pace of mechanistic discovery regarding how Alzheimer’s Disease and Related Dementia mutations cause disease.

3. Generic Name of Product: Chromium Next GEM cell kits

4. Purchase Description:

Chromium Next GEM Automated Single Cell 3' Library & Gel Bead Kit v3.1, 24 rxn Chromium Next GEM Automated Single Cell 3' Library & Gel Bead Kit v3.1, 4 rxn Chromium Next GEM Chip G Automated Single Cell Kit, 48 rxns Chromium Next GEM Chip G Automated Single Cell Kit, 16 rxns Dual Index Kit TT Set A 96 rxns

5. Salient characteristics:

Vendor must be able to provide these specifications:

- Delivers paired full-length T-cell receptor alpha and beta chain sequences at the single cell level using universal and unbiased amplification strategies, thus avoiding amplification and detection bias associated with complex multiplex PCR while revealing deeper biological complexity of antigen specificity on a per-cell basis than single-chain (e.g., TCRβ) methods.

- The incorporation of a unique molecular identifier (UMI) in the template switch oligo enables transcript quantification and further reduces systematic bias by controlling for non-biological SNVs or other variants introduced by PCR and sequencing error.

- Enables the detection of germline and somatic variation across full-length antigen receptor V(D)J gene sequences.

- Identifies both productive and non-productive recombination events as well as adaptive immune cells with more than one productive T-cell receptor alpha or beta chain.

- Maps transcription start sites.

- Generates single cell information from tens of thousands of transcripts per cell.

- Upgrades existing short read sequencers to generate cell by cell 3’ mRNA counts for thousands of cells per run.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

- Produces up to 100K individual nanoliter reactions coupled with ~750,000 unique molecular barcodes.

- Allows detection of differential gene expression between individual cells.

- Provides stand alone and turn-key analysis pipelines and visualization software, open-source data analysis tools for rapid processing of Illumina sequencing data.

- Enables single cell 3’ counting of mRNA at 100X reduction in price compared to commonly used single cell platforms.

- Prepares up to 80,000 cells for single cell library preparation in 7 minutes.

- Must be compatible with NIA 10X Chromium Connect automated instrument.

6. Quantity:

Item Description (or equal) Catalog No. Quantity

Chromium Next GEM Automated Single Cell 3' Library & Gel Bead Kit v3.1, 24 rxn 1000141 2.00

Chromium Next GEM Automated Single Cell 3' Library & Gel Bead Kit v3.1, 4 rxn 1000147 1.00

Chromium Next GEM Chip G Automated Single Cell Kit, 48 rxns 1000136 1.00

Chromium Next GEM Chip G Automated Single Cell Kit, 16 rxns 1000146 1.00

Dual Index Kit TT Set A 96 rxns 1000215 1.00

7. Delivery Date: Within 90 days after contract award.

Delivery Location:

National Institute on Aging 9000 Rockville Pike, Building T44 Bethesda, MD 20892