Purchase Description.pdf

Purchase Description

1. Statement of Need and Purpose:

The purpose of this acquisition is to acquire a multi-fiber photometry system for in vivo fluorescence detection in the brains of behaving animals. This equipment will expand Research in the Integrative Neuroscience Section’s (INS) capability to perform in vivo fiber photometry experiments to assess cell-type specific function and connectivity in mouse brain. The system will allow INS to perform simultaneuous fluorescecence recordings from up to 100 brain regions and/or animals, with both green and red emission channels, and an isosbestic control.

2. Background Information and Objective:

Research in the Integrative Neuroscience Section (INS) probes the neural circuit basis of normal working memory, and characterizes the neural circuit and working memory dysfunction in a mouse models of genetic susceptibility to schizophrenia and related disorders. INS members use in vivo physiology to record the function of discrete cells and circuits in freely moving mice, and use optogenetic tools to control this function in real-time during behavior. INS focuses on understanding the neural computations within, and circuit-level interactions between, the prefrontal cortex (PFC), hippocampus, and thalamus of the mouse. The ability to conduct optical recordings of fluorescent indicators of neural activity in the brains of freely moving rodents is required for the research conducted in the INS.

The purpose of this acquisition is to acquire a multi-fiber photometry system for in vivo fluorescence detection in the brains of behaving animals. This equipment will be critical for the central mission of the Integrative Neuroscience Section (INS) to study the neural basis of normal and disordered learning and memory processes. This equipment is required to expand the section’s capability to perform in vivo fiber photometry experiments to assess cell-type specific function and connectivity in the mouse brain.

3. Generic Name of Product:

Multi-Fiber Photometry System

4. Purchase Description:

The Contractor shall provide the NINDS INS with the hardware and software brand name or equal to Neurophotometrics FP 3002 Second Generation Multi-Fiber Photometry System with FOC_BF_200um/1.25mm hardware.

5. Salient characteristics

Quantity Essential Features / Salient Characteristics

1 Multi-Fiber Photometry System:

- Record, simultaneously, from up to 100 brain regions.

- Ultra-stable scientific LEDs at 470 nm (eg GCaMP), 560 nm (eg RCaMP), and 415 nm (isosbestic).

- Integrated knife’s edge image splitter and custom sCMOS camera. High sensitivity at 100hz.

- Integrated laser (450 nm) for optogenetics stimulation (red-shifted opsin compatible).

- TTL inputs and outputs.

- Ability to synchronize clocks across multiple devices.

- +/- 1 us resolution.

- Real-time processing.

- Built-in function generator for driving opto stim.

1 637 Fiber Coupled Laser:

- 637 Fiber Coupled Laser integrated into photometry system with removable adaptor.

100 Hardware:

- Black ceramic ferrule for increased efficiency and reduced autofluorescence.

- 1.25 mm ferrule diameter, 200 um fiber core diameter.

- NA= 0.37, length = 10 mm.

1 Service:Onsite Installation and Training:

- Onsite installation

- 1-2 days for training on the system

- Training shall include test recording of animals and test data on different types of experiments.

- Installation shall be performed by an authorized service provider of the equipment.

6. Quantity:

1 system

7. Delivery Date:

The contractor shall deliver the equipment within twelve (12) weeks after contract award.

Delivery must be FOB Destination and delivery must be performed during normal working hours between 8:00 AM to 5:00 PM EST Monday through Friday).

Installation and Training shall occur within 5 weeks of delivery.

Total of 17 weeks after receipt of order.

8. Period of Performance:

Base: 24 Months

The Contractor shall provide a two (2) year warranty on all equipment. All products shall be warranted to be free from material defects in workmanship or material under normal use and service for the warranty period. The contractor shall provide software upgrades during the warranty period. Periodic preventative maintenance visits cannot be included in warranty.

9. Option(s):

None