Statement_of_Work_3816402.pdf

Statement of Work

The Obesity and Diabetes Research Section (ODCRS) of the NIDDK in Phoenix performs research studies investigating the etiology of obesity and diabetes. Our recent work from protocol 08DKN140 “Predicting

Adaptive Thermogenesis” has uncovered a thrifty phenotype of less energy efficient, obese individuals who lose less weight during 6 weeks of supervised calorie restriction. The future success of dietary weight loss efforts may be influenced by the energy expenditure response to caloric restriction.

In order to further investigate the etiology of this phenotype, we are interested in determining if there are differences in baseline adipose and muscle tissue efficiencies and/or changes in these tissue efficiencies in the 12 volunteers from our pivotal study. In this regard, we hope to perform RNA sequencing (RNA-seq) of adipose and muscle tissue obtained through biopsy before and after weight loss. RNA-sequencing, or whole transcriptome shotgun sequencing, uses the capabilities of next-generation de-sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.

We do not have the capacity to perform RNA-sequencing at the ODCRS, and need to find a Laboratory that can perform the necessary screening for us. The lab will perform RNA-sequencing on samples from adipose and muscle tissue biopsies. In order to maintain the integrity of the data, analysis must be done at the same lab. The Lab will analyze samples from 12 volunteers, pre- and post-weight loss (48 samples total 24 adipose, 24 muscle). The lab will use a 2x100 bp Hi-seq module for 6 samples per lane with a total RNA 1 ug of each sample. Data will be provided to us in the form of an Excel spreadsheet within 60 days.