ATTACHMENT 5_SOW-APPENDIX 4.pdf

Appendix 4 - Upload Screen

NTP-EstrousData.xlsx

Example of Animal X Time data input screen

Finish reading Animal 101 slide before proceeding to Animal 102 animal bwt day1 day2 day3 day4 day5 day6 day7 day8 day9 day10 day11 day12

# 31.1 E E M D E E M D E E M D

# 27.4 E M D E E M D E E M D E

# 30.3 M D E E M D E E M D E E

# 31.7 E E D D D D E E E M D E

# 35.1 M D E E M D D E E M D E

# 31.8 M D E E M D E E M D E E

# 30.8 E E M D E E M D E E M D

# 32.4 E M D E E M D E E M D E

# 30.6 E E M D E E M D E E M D

# 28 D E E M D E E M D E E M

# 31.7 E M D E E M D E E M D E

# 30.7 E M D E E M D E E M D E

# 27.3 D E E M D E E M D E E M

# 34 M D E E E M D E E M D E

# 33 E M D E E M D E E M D E

# 33.2 E M D E E M D E E M D E

# 34.5 E E M D E E M D E E M D

# 27.8 E M D E E M D E E M D E

ATTACHMENT 5

Appendix 4 – Upload Screen NTP-ExtrousCyclingAlgorithm.xlsx input choices: P, E, M, D, NC, ND

NC = too few cells; ND = cannot determine stage is the female cycling?

starting with one of (P,E,M,D) does female return to initial stage? yes => cycling is observation period between 12 and 16 days?

yes => normal cycling call requires 2 cycles is the observation period 21 days?

yes => normal cycling call requires 3 cycles does the animal have only NC/ND between start and return? Yes => no cycling call is the cycle normal? normal length of each stage

RAT MOUSE

P 8H-12H 12H-2D

E 12H-2D 12H-3D

M 23H 21H-2D

D 2D 57H-3D

Yes = falls within specified ranges

Prolonged = stage is longer than normal range but animal proceeds to next stage

Persistent = stage is longer than normal and animal does not proceed to next stage cycle length = day after initial stage to first day of return to that stage EXAMPLE: EEMMDDE

..12345 qualitative:

cycle is regular: female has cycles with equal length =/- 1 day mean cycle length for female = average of cycle lengths for each female

Appendix 4 – Upload Screen NTP-ExtrousCyclingAlgorithm.xlsx cycle is irregular: female has cycles with different lengths mean cycle length for dose group = mean of individual mean cycle lengths, plus St Dev.