CSS_Attachment_No._01-Purchase_Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

This requisition is to acquire ACEA Biosciences xCELLigence RTCA CardioECR instrument, or a brand name equal item, for TRND biology.

2. Background Information and Objective:

Detecting muscle contractility is typically a very high-labor, low-throughput endeavor.

Techniques such as microscopy, manual patch-clamp and even high content plate readers can provide a wealth of information, but only for individual cells, and at an extremely low throughput. For that reason, these platforms are not suitable for our high-throughput laboratory or appropriate for the innovative mission of NCATS.

In recent years, there have been a number of improvements in the field, allowing the traditional patch-clamp method to be replaced with multiple point or whole well reads at a higher throughput that is more amenable to TRND. In fact, a few of the technologies have been evaluated and purchased by TRND and other laboratories at NCATS, including Axion MEAN, FLIPR, Opera and the FDSS 7000.

The newest technology has combined MEA and contractility recording allowing for a more comprehensive evaluation of excitation and contraction coupling. This technology is the first to simultaneous measure cardiomyocyte contractility and electrical activities in a 48-well plate format. The innovative pacing feature also allows for longer term measurement of cardiomyocyte viability, which has positive implications for important cytotoxicity screens performed at TRND.

3. Generic Name of Product:

Real-time measurement of cardiomyocyte contractility, viability, and electrical activity instrument.

4. Purchase Description: The NCATS requires one (1) brand name or equal real-time measurement of cardiomyocyte contractility, viability, and electrical activity instrument equivalent to the following:

• Brand name: xCELLigence RTCA CardioECR.

• Manufacturer: ACEA Biosciences, Inc.

• Catalog number: 380601210.

• 30.0 cm x 37.5 cm x 14.0 cm (W x D x H).

• Quantity: one (1).

5. Salient characteristics: The Brand name equal item must meet the following salient physical, functional, or performance characteristics of the brand name item to be acceptable for award:

Solicitation No. NIHDA201800513 Attachment No. 01

• The system should have complimentary shipping.

• The system should have complimentary installation.

• The system should have complimentary training.

• Instrument should not exceed the following dimensions: 30cm x 40cm x 15cm (W x D x H)

• The system should allow simultaneous impedance and field potential (FP) measurement while cardiomyocytes are being paced (stimulation range: ± 2.5 V).

• The system should have impedance-based, real time dynamic monitoring of beating cardiomyocytes on a 1-2 millisecond timescale.

• The system should provide real time, field potential measurement for the integrated ion channel activity of cardiomyocytes at 10 kHz sampling rate.

• The system should offer electrical pacing function.

• The system should able to be placed in a standard tissue culture incubator and withstand the high temperature and humidity.

• The software should be programmable to acquire beating data on a millisecond timescale, at intervals ranging from less than one minute to weeks for assessment of both acute and long-term cardio toxic effects.

• The system should have the ability to be electrically interfaced with a 48-well microtiter plate

• The system should be compatible plates with a standard footprint (complies with ANSI/SBS 1-2004 requirements, and the spacing of the wells in column is 9 mm center-to-center as per the ANSI/SBS 4-2004 standard for 96-well microplates).

• There should be two types of recording electrodes in each well: (1) two sets of interdigitated impedance electrodes enable real-time measurement cell viability and contractile activities. (2) two point field potential electrodes allow for extracellular field potential measurements, which can be performed in simultaneously with impedance recording.

• System plates should have the ability to be imaged through an electrode-free area using a standard laboratory microscope.

• System should come with fully integrated data acquisition software for operating the system. There should be flexible experiment setups, real time data acquisition, and basic data analysis functions such as – slope and Cell Index doubling time over a specific time period, EC50 (Half Maximal Effective Concentration) and IC50 (Half Maximal Inhibitory Concentration) values at a given time point, maximum Cell Index, minimum Cell Index, AUC (area under the curve) and time dependent IC50/EC50 determination, beating rate, beat amplitude, normalized beating rate/amplitude, and beating period/duration.

• Analysis Software should have an offline data analysis program for handling data acquired by the aquisition software.

• Analyis software should feature automatic report functions such as automatic statistics for cell status QC before compound addition, stable beating characteristics (including Cell Index, Maximal Cell Index, and AUC, beating rate and amplitude, Firing Rate and Amplitude).

• Analysis software should permit analysis of experimental data from different wells, time points and even from different experiments.

• The system should be able to measure cell proliferation, cell quality control, compound mediated cytotoxicity, cell-mediated cytotoxicity, cell adhesion and spreading, functional monitoring and receptor tyrosine kinase and GPCR (G Protein-Coupled Receptor) signaling, viral mediated cytopathic effect (CPE), parasite motility and biofilm formation.

• The system should not require calibration for proper function.

6. Quantity: One (1) xCELLigence RTCA CardioECR instrument, or brand name equal item.

7. Delivery Date: Within fifty (50) days after receipt of order (ARO). Delivery shall be made to:

ATTN: Wei Zheng

NCATS/NIH

9800 Medical Center Dr., Bldg. C Rockville, MD 20850

8. Option(s): N/A.

PURCHASE DESCRIPTION