Attachment_No._01-Purchase_Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Center for Advancing Translational Sciences (NCATS) requires a brand name or equal single-cell western blot system and two-color scanner equivalent to the following: ProteinSimple Milo and InnoScan 710.

The ProteinSimple Milo and InnoScan 710 two-color scanner package system is a single-cell western platform for the detection of protein expression in thousands of single cells in a single run. It allows for 1 minute SDS-PAGE separation on each single-cell lysate on-chip. The ProteinSimple Milo and two-color scanner package system provides more a single-cell proteome information than is possible with any other single-cell technique for use in analysis of pluripotent stem cells and differentiated counterparts.

2. Background Information and Objective:

The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability, conducting, supporting and making medical discoveries that improve people's health and save lives. The National Center for Advancing Translational Sciences (NCATS) is a translational science center that prides itself on its tremendously productive pipeline and is innovative in several ways. NCATS brings together a diverse range of scientists, including medicinal chemists, biologists, toxicologists, and engineers, in to ultimately translate basic science into real products and services that help improve people's lives.

To establish and move stem cell technologies forward through a more centralized effort, the National Institutes of Health (NIH) has launched the Stem Cell Translation Laboratory (SCTL) within NCATS. Part of the NIH Common Fund, the goal of the SCTL is to bring induced pluripotent stem cell (iPSC) technology closer to clinical application, drug discovery and regenerative medicine. Through the SCTL, NCATS will provide researchers across various disciplines and organizations with the ability to establish collaborations to advance the translation of regenerative medicine applications. The single-cell western blot system and two-color scanner is required to advance this mission.

RFQ No. NIHDA201800448 Attachment No. 01

3. The Generic Name of Product:

Single-Cell Western Blot System and Two-Color Scanner.

4. Purchase Description:

NCATS requires one (1) brand name or equal single-cell western blot system and two-color scanner equivalent to the following:

Brand name: Milo Single-Cell Western and InnoScan 710 with on-site training and systems training kit.

Manufacturer’s name: ProteinSimple Model, type, catalog number:

P300 (system and scanner), quantity: one (1).

A800 (training), quantity: one (1).

035-106 (training kit), quantity: one (1).

5. Salient Physical, Functional, or Performance Characteristics:

Brand name equal items must meet the following salient physical, functional, or performance characteristics of the brand name item to be acceptable for award:

• Ability to capture >1000 cells, lyse, and run an SDS-PAGE separation of every single cells and immobilize the separated proteins in a fully automated fashion, in less than 5 minutes.

• Includes image analysis software to quantify the acquired data.

• Must be compatible with cell diameters of 7-25um.

• Must allow single-cell protein separation of 15-175 kDa.

• Allow multiplexing of at least 4 proteins per single cell.

• Provides as low as 30% differences in same spectral channel.

• The accompanying microarray scanner must scan at least two colors ( 532nm and 635nm), a resolution of 3-40um/pixel, confocal detection, real-time autofocus and streamline compatibility with the analysis software.

• The Contractor must also provide hands-on training on the equipment.

• Include a system training kit with all the reagents necessary for a hands-on wet lab training session.

6. Quantity:

One (1) complete system package including on-site training and systems training kit.

7. Delivery Date:

Delivery shall be a one-time delivery of the system and training within one hundred twenty (120) days after receipt of order (ARO). Delivery shall be made to the below address FOB destination:

ATTN: Ilyas Singec

NCATS

9800 Medical Center Drive, Building B Rockville, MD 20850

Installation and on-site training shall occur shortly after delivery. The exact date and time will be determined by NCATS and the contractor.