SOW_Final.docx

STATEMENT OF WORK

ANALYSIS OF THREE OXIDATIVE STRESS BIOMAKERS

SECTION 1.0 – BACKGROUND

The purpose of this action is to procure services from the Eicosanoid Core Laboratory at Vanderbilt University for the analysis of three oxidative stress biomarkers in 1229 urine samples.

SECTION 2.0 OBJECTIVE

The purpose of this study is to examine oxidative stress biomarkers as a potential mediators in the relationship between environmental chemical exposures and fertility endpoints in men and women from the Environment and Reproductive Health Study (EARTH). Specifically, the goals are to investigate: 1) the association between phthalate exposure and oxidative stress biomarkers; 2) the relationship between oxidative stress biomarkers and semen quality; and 3) the relationship between oxidative stress biomarkers and antral follicle count, an indicator of female fertility.

SECTION 3.0 - SCOPE OF WORK

The following tasks shall be performed independent of Government supervision, direction, or control:

· a. The contract shall receive shipment of urine samples (n=1,229) from the Harvard TH Chan School of Public Health and store at -80 degrees C until analysis;

· b. Analyze 1,229 urine samples for concentrations of 8-iso-prostaglandin F2α, 2,3-dinor-5,6-dihydro-15-F2t-isoprostane (primary F2-isoprostane metabolite), and prostaglandin F2α via established gas chromatographic/negative ion chemical ionization mass spectrometric (GC/NICI‐MS) methods (see Milne et al. 2007) using appropriate quality control procedures;

· c. Re-freezing and storage of urine samples at -80 degrees C until analysis of all samples is complete;

· d. Quality control and assurance checks of collected data Transfer of clean data to Dr. Ferguson at NIEHS and collaborator Dr. Russ Hauser at Harvard TH Chan SPH

· Shipment of remaining sample volume back to Dr. Hauser

SECTION 4.0 – DELIVERABLES

a. The contractor will provide the analyzed clean samples back to the program office.

SECTION 5.0 – GOVERNMENT FURNISHED INFORMATION

N/A

SECTION 6.0 – GOVERNMENT FURNISHED EQUIPMENT

DIR will provide 1,229 urine samples to the contractor for analysis.

Period of performance is 3 months.

SECTION 7.0 – PERFORMANCE AND DELIVERY

Award need date: 01/03/2017

The written report and acquired data will be sent to Dr. Ferguson by email at kelly.ferguson2@nih.gov.

Upon completion of analysis, all analyzed urine samples shall be returned to sender at the Harvard TH Chan School of Public Health.

SECTION 8.0 – CONFERENCES AND MEETINGS

N/A

SECTION 9.0 – TRAVEL

SECTION 10.0 – SECURITY

N/A