Sources_Sought_Notice_N02RC54421_05.pdf

SOURCES SOUGHT NOTICE/REQUEST FOR INFORMATION:

If any responsible source believes it can provide equipment and software that meets the requirements they may submit their capability statements via email (PDF format; 15 Page Limit, these pages exclude resumes) for consideration by NCI to Heidi Crawley, Contracting Officer, at Heidi.crawley@nih.gov by the 9 am ET, July 22, 2015. No collect calls or facsimile transmissions will be accepted.

Notice Number:

SS‐ N02RC54421-05

Issued By:

National Cancer Institute (NCI), Office of Acquisitions (OA) http://www.nci.nih.gov

Key Dates:

Capability Statement Due Date: July 22, 2015 by 9:00 AM ET

This Small Business Sources Sought Notice (SBSS)/Request for Information is for information and planning purposes only and shall not be construed as a solicitation or as an obligation on the part of the National Cancer Institute (NCI).

The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) the availability of any source that believes it can provide equipment and software that meets the requirements; (3) whether sources are small businesses;

HUBZone small businesses; service‐disabled, veteran‐owned small businesses; 8(a) small businesses;

veteran‐owned small businesses; woman‐owned small businesses; or small disadvantaged businesses; and (4) sources' size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set‐ aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This requirement is assigned NAICS code 334516 with a size standard of 500 employees.

A. BACKGROUND

The National Cancer Institute (NCI) plans to procure a Flow Cytometry Cell Analyzer. The LGI Flow

Cytometry Core Facility in building 37 supports the Center for Cancer Research (CCR) scientific research community by providing instrumentation and expertise to perform state‐of‐the art cell analysis and cell sorting. The laboratory currently houses three cell sorters and four cell analyzers. These instruments are used daily serving over 200 scientists, roughly 70 different Principal Investigators annually. To maintain this facility at the forefront of flow cytometry technology and to accommodate increasing demands of CCR investigator Giorgio Trinchieri, M.D., Cancer and Inflammation Program Director, Laboratory of Experimental Immunology Chief and a new tenure track scientist in his laboratory, Romina Goldszmid, Ph.D. the facility requires the addition of this advanced, multi‐laser flow cytometry cell analyzer.

B. PURPOSE AND SALIENT CHARACTERISTICS

Contractor to provide Flow Cytometry Cell Analyzer with 21 parameters, designed to be expandable and capable of detecting 2 light scatter parameters and 18 fluorchromes simultaneously by 5 separate lasers. The proposed instrument will be further upgraded by removing the following parts; three photomultiplier tubes (PMTs), an octagon housing, Diva electronics (Card Cage, 5 DAQ, 5 SCI, 2‐Remote

Preamp) and optical filters needed to detect the Brilliant Violet fluorochromes BV650, BV711, BV786 and Sirigen filter and mirrors for the Violet laser.

Lasers:

Blue laser: solid‐state Coherent OBIS 488nm (60mw) with the ability to detect 2‐colors and Side Scatter

Red laser: solid‐state Coherent OBIS 640nm Red laser (40mw) with the ability to detect 3 colors

Yellow‐Green laser: solid‐state Coherent 561nm yellow‐ green laser (50mw) with the ability to detect 5 colors

Violet laser: solid‐state Coherent OBIS 405nm violet laser (100mw) with the ability to detect 6 colors

UV laser: solid‐state 355nm UV laser (60mw) with the ability to detect 3 colors from the UV laser.

Optical Filter Sets:

Blue laser:

o SSC o FITC (505LP, 530/30) o PerCP-Cy5.5 (685LP, 695/40)

Red laser:

o APC (670/30) o AF700 (710LP, 730/45) o APC-Cy7 (750LP, 780/60)

Yellow-Green laser:

o PE (586/15) o PE-Texas Red (600LP, 610/20) o PE-Cy5 (635LP, 670/30) o PE-Cy5.5 (685LP, 710/50) o PE-Cy7 (750 LP, 780/60)

Violet laser:

o BV421, DAPI (450/50) o BV510 (495LP, 525/50) o BV605 (595LP, 610/20) o BV650 (635LP, 670/30) o BV711 (695LP, 710/50) o BV786 (750LP, 780/60)

UV laser:

o BUV396 (379/28 o BUV496 (450LP, 515/30) o BUV737 (690LP, 740/35)

Additional Filter Set o BV805 (770LP, 820/60) o DAPI (410LP, 450/50)

Computer Components:

A Z230 computer workstation with Microsoft Windows 7 Professional, 32 bit OS US and two 19-inch NEC Color LCD Display Monitors with the following or better and is required;

Processor: Intel Xeon E3-1240v3 3.4 GHz (up to 3.8 GHz) 8MB 80W GT0 4C HT CPU RAM: 4GB DDR3-1600 ECC (2x2GB) Unbuffered RAM Video card: AMD FirePro V3900 1GB DL-DVI(I) Graphics 1st Hard drive: HP 500GB SATA 7200 1st HDD (1st slot) 2nd Hard drive: HP 1TB 7200 RPM SATA 2nd HDD (2nd slot) DVD drive: 16X SuperMulti DVDRW SATA 1st ODD 1st Ethernet Card: Integrated Gigabit (10/100/1000) Ethernet 2nd Ethernet Card: Intel Ethernet I210-T1 PCIe NIC A power conditioner is required to protect the computer and instrument in the event of a power surge.

Instrument Specifications:

Must have the capability of being operated by multiple users for a variety of applications in immunobiology and molecular immunology.

Must have the ability to customize and configure fluorescence detectors, including user access to, and ability to easily change the 25mm diameter fluorescence band pass filters and accompanying long pass filters in the filter holders designed to be interchangeable between the existing and proposed instrument.

Must have simultaneous detection of eighteen (18) fluorochromes from five (5) lasers, plus

Forward Scatter (FSC) and Side Scatter (SSC).

Must be upgradable to the following optional laser wavelengths and powers: 445nm-75mw, 514nm-20mw, 561nm-150mw, 685nm-40mw, 730nm-30mw, 750nm-20mw, and 785nm- 50mw.

Must have the ability to incorporate five (5) spatially separated lasers with automatic time delay calculation using instrument set-up software.

Must have variable detector configurations using reflected light trigon and octagon PMT arrays that use 25mm band pass filters.

Must have the capability to detect 80 MESF particles on the FITC channel.

Must have the capability of resolving .5um beads from noise on Side Scatter Channel.

Optical design must be able to deliver greater than 80% of stated laser power at the flow cell.

Must be able to have a Fluorescence Linearity Doublet/Singlet Ratio of 1.95-2.05 for CEN Stained with PI.

Must incorporate all digital electronics to provide data acquisition rates up to 40,000 events per second with beads and 10,000 events per second with cells.

Must incorporate manual (not motor driven) sample change capability, and provide for sample carryover of less than 0.1% within 5 seconds.

Must have integrated laser power control software that provides for turning power on/off and adjustable power level control for each of the 5 lasers independently.

Must have five (5) lasers with the following wavelength (nm) and power output maximum (milliwatts):

1. 405 nm, 100 milliwatts

2. 488 nm,100 milliwatts

3. 640 nm, 100 milliwatts

4. 561nm, 150 milliwats

5. 355nm, 15 milliwats

Must be able to support installation of BD High Throughput Sampler (HTS) which uses a fully integrated automatic sample injection from microliter plates (96 or 384 well) with operation controlled by the cytometer software.

Must use BD FACSDiva 8.0 or higher for data acquisition and analysis on the instrument.

Must include a table with heavy wheels capable of supporting the instrument.

The selling price of the requested instrument includes the trade-in of BD LSRII S/N #H1076.

WARRANTY:

Twelve-month warranty will begin upon installation or thirty days after the date of shipment, whichever occurs first.

TRAINING:

Purchase price for each system shall include two training provisions. Training must take place within one year from the date of installation. The contractor shall provide lodging, breakfasts at the hotel, and lunches at the Training Center during the training.

C. HOW TO SUBMIT A RESPONSE

If any responsible source believes it can provide equipment and software that meets the requirements they may submit their capability statements via email (PDF format; 15 Page Limit, these pages exclude resumes) for consideration by NCI to Heidi Crawley, Contracting Officer, at Heidi.crawley@nih.gov by the 9 am ET, July 22, 2015. No collect calls or facsimile transmissions will be accepted.

Respondents must include DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HUBZONE, etc) pursuant to the applicable NAICS code.

Disclaimer and Important Notes:

This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, an RFQ may be published on FedBizOpps.

However, responses to this notice will not be considered adequate responses to a solicitation(s).

Point of Contact:

Inquiries concerning this Notice may be directed to:

Heidi Crawley Contracting Officer 301‐624‐8764 Heidi.crawley@nih.gov