PreSol_Notice_Digital_PCR_System.pdf

Presolicitation Notice

Notice Number:

N02CO47038-50

Issued By:

National Cancer Institute (NCI), Office of Acquisitions (OA) http://www.nci.nih.gov

Key Dates:

Posted Date: July 10, 2014 Response Date: July 25, 2014 at 10:00 AM ET

This requirement is assigned NAICS code 334516 with a size standard of 500 employees.

A. Description:

The National Cancer Institute (NCI) plans to procure two Digital PCR Systems. The system will be housed in the CCR Genomics Core, and the other within the Genetics Branch, both are part of the

Center for Cancer Research, NCI. The Contractor shall provide a platform that shall partition each ddPCR reaction mix into 20,000 nanoliter-sized droplets, stream emulsified droplets in single file past a two-color optical detection system in a serial manner, as well starting accessories like oil for setup of the instruments.

B. PURPOSE AND SALIENT CHARACTERISTICS

The purpose of this acquisition is to purchase an Droplet Digital PCR System

Automated Droplet Generator:

• Automatic droplet generator to partition a 20ul PCR reaction into 20,000 nano-liter sized droplets, and generation is in 2 minutes.

• Droplet generation should require generator oil for each reaction.

• Generator should be able to support automated droplet generation from up to 96 samples simultaneously, while also being compatible with processing smaller numbers of samples.

• Process of droplet generation should be automated, and include a liquid handling system to accurately pipet reagents and samples to reduce user-to-user variability and to save time.

• Instrument should be enclosed with appropriate filters to reduce contamination.

• System should be compatible with both Taqman hydrolysis and EvaGreen interchelating

DNA dye chemistries.

• Each droplet is 1nl and is approximately 120um.

Droplet Reader:

• Droplet reader analyzes a full 96-well plate in less than 2.5hours.

http://www.nci.nih.gov/

• Droplet reader performs excitation and detection of up to two fluorescent dyes per droplet.

FAM, HEX (or VIC), and EvaGreen dyes can be used.

• Droplet reader illuminates and detects fluorescence from each individual droplet. The system should illuminate and differentially detect emission using two filtered counters, one for each fluorescent channel.

• Droplet reader automatically detects fluorescence from both channels during the reading process.

• System has a linear dynamic range of 5 orders of magnitude for detection of input samples.

• System can detect one copy of target sequence from DNA samples.

• System enables distinction between four and five copies of input target nuclear acid between samples.

• Precision of +/- 10% can be achieved.

• One 96-well plate can be used to analyze up to 1.5million droplets.

• System enables distinction between four and five copies of input target nuclear acid between samples.

• Precision of +/- 10% can be achieved.

• Compatible with both TaqMan probe chemistry and interchelating DNA binding chemistry like EvaGreen.

• DNA from unread droplets can be recovered for downstream applications.

• One 96-well plate can be used to analyze approximately 1.5million droplets.

Software:

• Displays fluorescence amplitude values per droplet for FAM, HEX (or VIC) and EvaGreen dyes.

• Shows multiples data for up to two fluorophores per droplet.

• Computes concentration (copies/ul) for each sample.

• Calculates copy number for target of interest using reference target for copy number variation analysis.

• Calculates fractional abundance of mutant target in wildtype background for mutation detection.

• Software should automatically set thresholds across the entire plate or for individual wells, but users should also be able to set thresholds manually.

• Should be able to merge results from replicate wells.

• Should determine absolute copy number of target DNA, taking into account the fraction of negative droplets.

• Allows data to be exported as a .csv file (for use in Excel or other programs).

• Allows graphs and tables to be copied to clipboard for printing from software menus.

Plate Sealer:

• A single plate support block that can hold a 96- and 384-well plates, to aid in heat sealing.

• Provides consistent and uniform sealing across an entire microplate.

• Semi-automated heat sealer ready to seal in less than 5 minutes.

• Minimizes sample evaporation from the PCR workflow and is compatible with a variety of heat sealing films and foils and a wide range of PCR plates.

• Should have an intuitive touch-screen user interface.

• Allow sealing temperature and time to be modified with minimal effort.

C1000 Thermal Cycler:

• Able to accommodate different throughput needs with easily interchangeable reaction modules.

• Each PCR module has a fully adjustable heated lid that supports a wide range of vessels and sealers, including low-profile and standard-height PCR plates.

• Quick and easy protocol programming, to enable easy generation of customized standard, fast, and ultrafast protocols.

• Has an advanced file management system using a USB flash drive compatibility to allow universal protocol transfer and unlimited data storage.

• Can quickly and easily identify optimal annealing temperatures by use of the programmable temperature gradient.

The NCI CCR anticipates award of a firm fixed price, non-competitive order to BioRad Laboratories to provide the equipment and accessories described in Section B. Only BioRad Laboratories has the equipment and software to perform the tasks describe above.

The supplies herein are being procured in accordance with the simplified acquisition procedures as authorized by FAR Part 13.106-1 (b)(1). Sources Sought Notice SS-N02CO47038-50 was posted on

FedBizOpps.gov on 6/25/2014 and did not generate any responses. The North American Industry

Classification System code is 334516 and the business size standard is 500 employees.

The equipment shall be purchased within 1 week after the close of this Presolicitation Notice.

This procurement will purchase the following equipment:

DESCRIPTION QUANTITY

QX200 Digital PCR Reader 2 each

QX200 Droplet Generator 2 each

Automated Droplet Generator 2 each

Laptop Computer for ddPCR system 2 each

PX1 PCR Plate Sealer 1 each

Droplet Reader Oil 2 each

C1000 Touch Cycler w/96W DP RM 1 each http://www.bio-rad.com/evportal/destination/product?catID=M9ZRW9E8Z http://www.bio-rad.com/evportal/destination/product?catID=027f88f2-615e-424a-803a-9baa28b55cab

This notice is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement. Capability statements must be received in the contracting office by 10:00 AM ET, on July 25, 2014. For further information, please contact Andrew May, Contracting Officer via electronic mail at Andrew.May@nih.gov. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government.

Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. No faxed or emailed capability statements will be accepted. In order to receive an award, contractors must be registered and have valid certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA) thru sam.gov. Reference: N02CO47038-50 on all correspondence.

Inquiries concerning this Notice may be directed to:

Andrew May Contracting Officer 301-624-8747 Andrew.May@nih.gov