SF_18_Request_for_Quotation_4241155.pdf

REQUEST FOR QUOTATION

(THIS IS NOT AN ORDER)

THIS RFQ IS IS NOT A SMALL BUSINESS SET-ASIDE

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IMPORTANT: This is a request for information and quotations furnished are not offers. If you are unable to quote, please so indicate on this form and return it to the address in Block 5a. This request does not commit the Government to pay any costs incurred in the preparation of the submission of this quotation or to contract for supplies or service. Supplies are of domestic origin unless otherwise indicated by quoter. Any representations and/or certifications attached to this Request for Quotation must be completed by the quoter.

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4241155NIDA-2016-RFQ-543

NIH, National Institute on Drug Abuse (NIDA), SSSA Branch

Danielle R. Brown

This is a request for quotation to provide deep sequencing services of DNA, RNA, chromatin immunoprecipitation (ChIP), and ATAC sequencing of samples obtained from primary human peri-pheral blood cell subsets.

Deep sequencing will be carried out with an up-dated Hi-Seq machine to identify low-abundance intergenic non-coding RNAs and examine epigenetic changes in primary human peripheral blood cell subsets.

The deep sequencing will begin immediately following the award.

301 480-2385 National Institute on Drug Abuse

6001 Executive Boulevard

Bethesda

20892MD

09/08/2016

09/13/2016

09/30/2016

HHSN-NIH-NIDA-SSSA-RFQ-2016-543

REQUEST FOR QUOTATION

Immunosenescence and Inflammation Associated with Aging.

REQUEST NUMBER: HHSN-NIH-NIDA-SSSA-RFQ-2016-543

DATE ISSUED: September 8, 2016

ISSUED BY CONTRACTING OFFICE: National Institute on Drug Abuse (NIDA), Neurosciences Consolidated Operation Acquisition Center (COAC), Office of Acquisition (OA), Station Support Contracts and Simplified Acquisition Branch (SS/SA)

FOR INFORMATION CONTACT: Danielle R. Brown at Danielle.brown2@nih.gov and (301) 480-2385.

NORTH AMERICAN CLASSIFICATION SYSTEM (NAICS) CODE: 541380 – Testing Laboratories

SMALL BUSINESS SIZE STANDARD: $15 Million

SET-ASIDE STATUS: There is no set-aside status; other than small business.

Competition Status: This requirement is non-competitive.

Non-Competitive (Single-sole Source (Including brand-name)) Determination:

This notice of proposed acquisition is posted as an intent to award a purchase order on a non-competitive basis to the Johns Hopkins Microarray Facility located on the 3rd floor of the Miller Research Building (MRB), 733 North Broadway, Room 351, Baltimore, MD 21205.

DATE QUOTATION DUE: Monday, September 12, 2016 at 12 Noon All responses to this request for quote must be received by Monday, September 12, 2016 at 12 Noon Eastern Standard Time and must reference the request number cited above. Responses must be submitted electronically to the contact person cited above. Fax responses will not be accepted.

IMPORTANT: This is a request for information and quotations furnished are not offers. If you are unable to quote, please so indicate in a reply email to the contract specialist cited herein.

This request does not commit the Government to pay any costs incurred in the preparation of the submission of this quotation or to contract for supplies or service. Supplies are of domestic origin unless otherwise indicated by quoter. Any representations and/or certifications attached to this Request for Quotation must be completed by the quoter.

mailto:Danielle.brown2@nih.gov

Description of Requirement:

The requested procurement order is to contract deep sequencing of RNA and chromatin immunoprecipipitation (chip) cell subsets obtained from human peripheral blood mononuclear cells from volunteers for ongoing projects (BLSA, GESTALT, normal donors). Additionally, deep sequencing will be carried out to measure chromatin accessibility changes with age and chromatin changes conformational changes (ChIA-Pet).

The proposed project will examine age-associated changes in gene expression and epigenetic state of 10 cell subsets purified from human peripheral blood. This is a collaborative venture that involves several NIA/IRP laboratories, including, LMBI, TGB, LMG, and LG. Cytapheresis packs will be collected from 30 volunteers of varying age-groups and cells fractioned by a combination of magnetic bead purification and flow cytometry. These cells will be used to isolate RNA and carry out ChIP and ATAC. Purified RNA and DNA will be provided to Dr. Hao for deep sequencing. Additionally, ChIA-Pet will be used to obtain chromatin structural information. ATAC and ChIA-Pet libraries will be prepared at the NIA and provided to the contractor.

DESCRIPTION

1) DNA Seq

a. Quantity – 36

b. Description – Sequence ATAC-Seq/ChIA-pet library up to 130 million paired end

2x75bp

2) RNA Seq

a. Quantity – 60

b. Description – RNA QC, Truseq stranded mRNA library preparation, and sequence to 20-25 million single 50 bp reads per sample

3) ChIP Seq

a. Quantity – 30

b. Description DNA QC, library preparation with barcodes, sequenced to approximately 20-25 million single 50 bp reads

QUOTER TERMS AND CONDITIONS:

1. Payment Discount Terms:

2. Open Market; GSA FSS Contract Number; Other Government Agency (OGA) Contract

Number, Government-wide Agency (GWAC) Contract Number; or National Institute of Health (NIH) Blanket Purchase Agreement (BPA) Number:

3. DUNS No:

4. Name of Company:

5. Street Address, City, State, Zip code:

6. Name of Person Authorized to Provide Quote:

7. Telephone Number and Email Address of Person Authorized to Provide Quote:

ADDITIONAL TERMS AND CONDITIONS, PROVISIONS, AND REPRESENTATIONS

The FAR Clauses are incorporated by reference and are provided in full text at https://www.acquisition.gov/

1. The provisions of FAR Clause 52.212-1 Instructions to Offerors—Commercial Items (Oct

2015) is applicable to this solicitation.

2. The provision of FAR Clause 52.212.2 Evaluation—Commercial Items (Oct 2014) is applicable to this solicitation. Lowest price technically acceptable approach will be used to evaluate quotes.

3. The provisions of FAR Clause 52.212-3, Offeror Representations and Certifications— Commercial Items (July 2016), is applicable to this solicitation.

4. The provision of FAR Clause 52.212-4, Contract Terms and Conditions—Commercial Items (May 2015) is applicable to this solicitation.

5. The provisions of FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders—Commercial Items (July 2016) is applicable to this solicitation. There are no specific clauses applicable to this solicitation.

SUMMARY STATEMENT:

Responses to this notice must include sufficient information to establish the interested parties’ bona-fide capabilities of providing the product or service. The response shall include: the unit price, list price, shipping and handling costs, delivery days after contract award, delivery terms, prompt payment discount terms, F.O.B. Point (Destination or Origin), product or catalog number(s); product description; and any other information or factors that may be considered in the award decision. Such factors may include: past performance; special features required for effective program performance; trade-in considerations; probable life of the item selected as compared with that of a comparable item; warranty considerations; maintenance availability;

and environmental and energy efficiency considerations.

https://www.acquisition.gov/