Questions_and_Answers_Amendment_0002.pdf

Solicitation: FDA_19-223-SOL-1211358 Amendment: 0002

Summary: The purpose of this amendment is to answer Contractor-posed question to the solicitation. The Government is providing responses below to all Contractor-posed questions received through April 25, 2019.

The questions posed does merit extending the response deadline. As a result, the response date will be extended from April 26, 2019 to May 3, 2019.

Questions and Answers:

Question #1: Can you provide the individual model descriptions for each of the individual model descriptions for each microscopes

Answer #1:

1. Zeiss CellObserver SD confocal microscope with TIRF: This system has Yokogawa CSU- X1 spinning disk confocal scan head with Zeiss TIRF (Total Internal Reflection Fluorescence) module. This system is equipped with Photometrics eVOLVE EMCCD camera and PECON large environmental chamber and used for live cell and tissue imaging.

2. Zeiss LSM710 NLO, point confocal multiphoton microscope: This system is equipped with custom motorized stage and Coherent chameleon infrared laser. This system is used for intra vital multiphoton microscopy for live tissue and small animal imaging.

3. Zeiss LSM710 34 channel confocal microscope system: This system is used for multicolor spectral confocal imaging for cell and tissue.

4. Zeiss Libra 120 transmission electron microscope: This electron microscope is used to obtain ultrastructure of virus and cells.

5. Zeiss LSM700 confocal microscope with Ultraviolet 405 laser: This system is used for multicolor confocal imaging with UV laser.