FBO_4_Crispr_Mouse.pdf

DESCRIPTION: The National Institutes of Health- NIAAA intends to procure the services of a contractor to provide CRISPR services to genetically modify a mouse.

It is the intent of the National Institutes of Health- NIAAA under the authority of FAR 6.302-1 to procure this service on a sole source.. The proposed contract action is for a “continuation of / follow-on similar services for which the Government intends to solicit and negotiate with only one source under the authority of FAR 6.302. In order to comply with the requirements in this Special Notice # NICHD-22- 140, the responses should be based on our interpretation of the general requirements provided below However, interested persons may identify their interest and capability to respond to the requirement or submit quotations. This notice is not a request for competitive quotes but all quotes received within the time and date of publication of this " Notice” will be considered and evaluated by the Government. A determination by the Government not to compete with this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a future competitive procurement.

The Lab has been working on the CRISPR /Cas 9Gene Editing services with Jackson Laboratory for their research over the years and changing the method would cost time and funds to re-start the project using another vendor’s method. The Jackson Laboratory will generate and insert CRISPR Knock In plasmid, pCAG-Lox-STOP-Lox-mouse Adgrf1-IREStdTomato-bGHpolyA into the C57BL/6J mouse ROSA26 locus to enhance the Adgrf1 expression. This service includes plasmid design and reagent preparation (8- 10 weeks), embryo manipulation (4-5 weeks) and founder identification (4-6 weeks), CRISPR breeding service and sperm cryopreservation. The Jackson Laboratory mice are the most well-characterized and widely used mice available, and once the sperm cryopreservation process has been completed, the straws of sperm will be maintained in liquid nitrogen storage in three separate tanks at The Jackson Laboratory.

HOW TO RESPOND: In order to compete for this project, interested parties must be registered in the Central Contracting Registration/SAM and submit a price quotation by 3:00 pm, ET on June 21, 2022.

Offerors should submit one copy of the quote to MOE FILALI via email. Electronic transmissions to Moe.Filali@nih.gov are preferable but must be timely. It is the responsibility of the Offeror to ensure the proposal is received by the date and time shown above.

This is a “special Notice”/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is NICHD-22-140 and is issued as a Request for Quotation (RFQ). The solicitation/contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2019-05.

The offeror must include a completed copy of the provision of FAR Clause 52.212-3, Offeror Representations and Certifications – Commercial Items with its offer. The provisions of FAR Clause 52.212-1 Instructions to Offerors – Commercial Items; FAR Clause 52.212-2, Evaluation – Commercial Items; FAR Clause 52.212-4, Contract Terms and Conditions – Commercial Items; and FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders – Commercial Items

– Deviation for Simplified Acquisitions applies to this acquisition. FAR Clause 52.204-24, Representation Regarding Certain Telecommunication and Video Surveillance Services or Equipment;

FAR Clause 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment; FAR Clause 52.213-4 to reflect the changes in FAC 2019-05 and FAR Clause 52.244-6 to reflect the changes in FAC 2019-05. The offeror must include their Dun &

Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. The clauses are available in full text at http://www.arnet.gov/far. Interested vendors capable of providing the Government with the equipment specified in this synopsis should submit their quotation to the below address. The quotation must reference “Solicitation number”. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. Quotations must be submitted by email to Moe.filali@nih.gov.

The following Federal Acquisition Regulations clauses are incorporated by reference: FAR 52.212-3, Offeror Representations and Certifications-Commercial Items (Mar 2005), 52.212-4 (Sept 2005), 52.212- 5, Contract Terms and Conditions Required to Implement Statutes of Executive Orders-Commercial Items (Aug 2006). (6)(i) 52.222-26, Equal Opportunity (Apr 2002) (E.O. 11246). (18) 52.222-35, Equal Opportunity for Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (19) 52.222-36, Affirmative Action for Workers with Disabilities (Jun 1998) (29 U.S.C. 793). (20) 52.222-37, Employment Reports on Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (31) 52.232-33, Payment by Electronic Funds Transfer-Central Contractor Registration (Oct 2003) (31 U.S.C. 3332).

http://www.arnet.gov/far