Attachment_1_-_SOW.pdf

Statement of Work

DNA Sequence Analysis Using Illumina Next-Generation Sequencers

1. Background

The United States Air Force School of Aerospace Medicine (USAFSAM) has a requirement for vendor supported reagents / kits necessary to perform DNA sequence analysis using Illumina Next-Generation Sequencers (NGS) such as the HiSeq 2500, MiSeq and the MiSeqDX. The reagents are required to support various DHP-funded research projects requiring sequence analysis. These projects supports the mission at the USAFSAM by providing continual evaluation, validation, and transition assistance with new off-the-shelf technologies and genomics technologies. The evaluation of improved methods will help fill in critical gaps in troop protection, diagnostics, epidemiology and preventative medicine. The goal of these research studies is to evaluate potential causes for infections in the population by sequencing various samples to include environmental, human and entomological.

The results will be utilized to determine the most prevalent causative agent(s) in the tested samples and to determine failure rate of traditional testing.

2. Description of Effort

2.1. Objective

The primary objective of this effort is to allow for the rapid procurement of ALL vendor reagents and kits associated with the HiSeq 2500, MiSeq and the MiSeqDX sequencers in order to meet existing and future research protocols over the course of the next five (5) years.

Due to the rapid advancements in the field of genomic sequencing, it is highly anticipated that over the course of the next five (5) years, there will be numerous item/part number changes and new reagents/kits offered, to replace older products, as a result of product advancement. Paramount to achieving the primary objective is the ability to rapidly deliver supplies subsequent to item/part number changes as well as the addition of new reagents/kits offered by the vendor.

2.2. Requirements

Upon receipt of specific product requirements, the vendor shall deliver kits, reagents, and supplies associated with

HiSeq 2500, MiSeq and the MiSeqDX NGS systems with a minimum remaining shelf life of three (3) months.

Manufacturer Catalog Number Description

Illumina FC-401-3001 TruSeq SBS Kit v3-HS (200 cycles)

Illumina PE-401-3001 TruSeq PE Cluster Kit v3-cBot-HS

Illumina FC-402-4023 HiSeq Rapid SBS Kit v2 (500 cycles)

Illumina FC-401-3002 TruSeq SBS Kit v3-HS (50 cycles)

Illumina FC-402-4021 HiSeq Rapid SBS Kit v2 (200 cycles)

Illumina PE-402-4002 HiSeq PE Rapid Cluster Kit v2

Illumina GD-402-4002 HiSeq SR Rapid Cluster Kit v2

Illumina FC-402-4022 HiSeq Rapid SBS Kit v2 (50 cycles)

Illumina MS-102-2023 20-pack MiSeq Reagent Kit v2 (500-cycles)

Illumina MS-102-2022 20-pack MiSeq Reagent Kit v2 (300-cycles)

Illumina MS-102-2021 20-pack MiSeq Reagent Kit v2 (50-cycles)

Illumina MS-102-3003 MiSeq Reagent Kit v3 (600-cycle)

Illumina MS-102-2003 MiSeq Reagent Kit v2 (500-cycles)

Illumina MS-102-2002 MiSeq Reagent Kit v2 (300-cycles)

Illumina MS-102-3001 MiSeq Reagent Kit v3 (150-cycle)

Illumina MS-102-2001 MiSeq Reagent Kit v2 (50-cycles)

Illumina MS-103-1002 MiSeq Reagent Micro Kit v2 (300-cycles)

Illumina MS-103-1003 MiSeq Reagent Nano Kit v2 (500-cycles)

Illumina MS-103-1001 MiSeq Reagent Nano Kit v2 (300-cycles)

Illumina FC-151-1003 TruSeq-Methyl Capture EPIC Library Prep Kit (48 samples, 12 indexes)

Illumina FC-140-1003 Nextera Rapid Capture Exome (8 rxn x 12 plex)

Illumina RS-122-2203 TruSeq Stranded Total RNA Library Prep Kit High Throughput (96 samples, 96 indexes)

Illumina RS-122-2303 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)

Illumina RS-122-2503 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin High Throughput (96 samples, 96 indexes)

Illumina FC-144-1004 TruSeq Rapid Exome Library Prep Kit (96 samples)

Illumina FC-140-1005 Nextera Rapid Capture Expanded Exome (4 rxn x 12 plex)

Illumina FC-150-1006 TruSeq® Expanded Exome Kit (8rxn × 12 plex)

Illumina FC-140-1089 Nextera Rapid Capture Exome (8 rxn x 9 plex)

Illumina FC-140-1002 Nextera Rapid Capture Exome (4 rxn x 12 plex)

Illumina RS-301-2001 TruSeq RNA Access Library Prep Kit - Set A (12 indexes, 48 samples)

Illumina RS-301-2002 TruSeq RNA Access Library Prep Kit - Set B (12 indexes, 48 samples)

Illumina FC-121-1031 Nextera DNA Library Prep Kit (96 samples)

Illumina FC-144-1003 TruSeq Rapid Exome Library Prep Kit (72 samples)

Illumina FC-150-1004 TruSeq Exome Library Prep Kit (96 samples)

Illumina RS-304-1002 TruSight RNA Fusion Panel Set A (48 samples)

Illumina RS-304-1003 TruSight RNA Fusion Panel Set B (48 samples)

Illumina FC-140-1004 Nextera Rapid Capture Expanded Exome (2 rxn x 12 plex)

Illumina RS-122-2202 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set B (48 samples, 12 indexes)

Illumina RS-122-2201 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set A (48 samples, 12 indexes)

Illumina RS-122-2301 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set A (48 samples, 12 indexes)

Illumina RS-122-2302 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set B (48 samples, 12 indexes)

Illumina RS-122-2501 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin Set A (48 samples, 12 indexes)

Illumina RS-122-2502 TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin Set B (48 samples, 12 indexes)

Illumina FC-140-1086 Nextera Rapid Capture Exome (8 rxn x 6 plex)

Illumina FC-140-1001 Nextera Rapid Capture Exome (2 rxn x 12 plex)

Illumina FC-150-1003 TruSeq Exome Library Prep Kit (72 samples)

Illumina FC-144-1002 TruSeq Rapid Exome Library Prep Kit (48 samples)

Illumina RS-122-2103 TruSeq Stranded mRNA Library Prep Kit High Throughput (96 samples, 96 indexes)

Illumina FC-150-1005 TruSeq® Expanded Exome Kit (8rxn × 6 plex)

Illumina RS-122-2001 TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)

Illumina FC-132-1001 Nextera Mate Pair Library Prep Kit (12 indexes, 48 gel-free samples or 12 gel-plus samples)

Illumina RS-122-2002 TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)

Illumina RS-122-2001 TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)

Illumina FC-150-1002 TruSeq Exome Library Prep Kit (48 samples)

Illumina FC-151-1002 TruSeq-Methyl Capture EPIC Library Prep Kit (12 samples, 4 indexes)

Illumina FC-126-1003 TruSeq Synthetic Long-Read DNA Barcode Kit (4 barcode plates)

Illumina FC-131-1096 Nextera XT DNA Library Preparation Kit (96 samples)

Illumina FC-121-3003 TruSeq DNA PCR-Free HT Library Preparation Kit (96 indexes in plate format, 96 samples)

Illumina FC-121-4003 TruSeq Nano DNA HT Library Preparation Kit (96 indexes in plate format, 96 samples)

Illumina FC-140-1103 TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)

Illumina IP-202-1024 TruSeq ChIP Sample Preparation Kit - Set B (12 indexes, 48 rxns)

Illumina IP-202-1012 TruSeq ChIP Library Preparation Kit - Set A (12 indexes, 48 rxns)

Illumina RS-122-2101 TruSeq Stranded mRNA Library Preparation Kit Set A (48 samples, 12 indexes)

Illumina RS-122-2102 TruSeq Stranded mRNA Library Preparation Kit Set B (48 samples, 12 indexes)

Illumina FC-140-1000 Nextera Rapid Capture Exome (8 rxn x 1 plex)

Illumina FC-140-1083 Nextera Rapid Capture Exome (8 rxn x 3 plex)

Illumina 20015963 TruSeq® DNA PCR-Free HT Library Preparation Kit (96 samples)

Illumina 20015965 TruSeq® Nano DNA HT Library Preparation Kit (96 samples)

Illumina 20014279 SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)

Illumina RS-200-0012 TruSeq Small RNA Library Prep Kit -Set A (24 rxns) (Set A: indexes 1-12)

Illumina RS-200-0024 TruSeq Small RNA Library Prep Kit -Set B (24 rxns) (Set B: indexes 13-24)

Illumina RS-200-0036 TruSeq Small RNA Library Prep Kit -Set C (24 rxns) (Set C: indexes 25-36)

Illumina RS-200-0048 TruSeq Small RNA Library Prep Kit -Set D (24 rxns) (Set D: indices 37-48)

Illumina FC-144-1000 TruSeq Rapid Exome Library Prep Kit (8 samples)

Illumina FC-144-1001 TruSeq Rapid Exome Library Prep Kit (24 samples)

Illumina FC-121-1030 Nextera DNA Library Prep Kit (24 samples)

Illumina FC-140-1101 TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)

Illumina FC-150-1001 TruSeq Exome Library Prep Kit (24 samples)

Illumina FC-140-1102 TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)

Illumina RT-402-1001 TruSeq Targeted RNA Index Kit A (96 indexes, 384 samples)

Illumina RT-402-1002 TruSeq Targeted RNA Index Kit B (96 indexes, 384 samples)

Illumina RT-402-1003 TruSeq Targeted RNA Index Kit C (96 indexes, 384 samples)

Illumina RT-402-1004 TruSeq Targeted RNA Index Kit D (96 indexes, 384 samples)

Illumina FC-121-1012 Nextera Index Kit (96 indexes, 384 samples)

Illumina FC-131-2001 Nextera XT Index Kit v2 Set A (96 Indexes, 384 Samples)

Illumina FC-131-2002 Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

Illumina FC-131-2003 Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

Illumina FC-131-2004 Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

Illumina FC-131-1024 Nextera XT DNA Library Preparation Kit (24 samples)

Illumina FC-121-3002 TruSeq DNA PCR-Free LT Library Preparation Kit - Set B (12 Set B index tubes, 24 samples)

Illumina FC-121-3001 TruSeq DNA PCR-Free LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)

Illumina FC-121-4001 TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)

Illumina FC-121-4002 TruSeq Nano DNA LT Library Preparation Kit - Set B (12 Set B index tubes, 24 samples)

Illumina RT-401-1001 TruSeq Targeted RNA Index Kit (48 indexes, 48 samples)

Illumina 20015962 TruSeq® DNA PCR-Free LT Library Preparation Kit (24 samples)

Illumina 20015964 TruSeq® Nano DNA LT Library Preparation Kit (24 samples)

Illumina 20015948 TruSeq® DNA Index kit Plate v2 (96 indexes, 96 samples)

Illumina 20015949 TruSeq® DNA Index kit Plate (96 indexes, 96 samples)

Illumina FC-121-1011 Nextera Index Kit (24 indexes, 96 samples)

Illumina 20015960 TruSeq® DNA Index kit Set A (12 indexes, 24 samples)

Illumina 20015961 TruSeq® DNA Index kit Set B (12 indexes, 24 samples)

Illumina EGMK91396 TruSeq DNA Methylation Kit (96 reactions)

Illumina MRZB12424 Ribo-Zero rRNA Removal Kit (Bacteria) 24 reactions

Illumina MRZE724 rRNA Removal Kit (Epidemiology) 24 reactions

Illumina EGMK91324 TruSeq DNA Methylation Kit (24 reactions)

Illumina FC-110-3001 PhiX Control v3

Illumina FC-110-3002 NextSeq™ PhiX Control Kit

Illumina WG-316-1004 Infinium Multi-Ethnic Global-8 v1.0 Kit (384 Samples)

Illumina 20015239 Infinium PsychArray-24 v1.2 Kit (288 samples)

Illumina SC-201-1001 Infinium High-Speed Microplate Shaker (110/120V)

Illumina BD-60-601 Infinium MIDI Heatblock Insert

Illumina BD-60-402 Infinium Hybridization Chamber (1)

3. Delivery Schedule

The vendor shall deliver all items requested within seven (7) days after receipt of the order. These items will have a minimum remaining shelf life of no less than three (3) months. The Government understands that order volumes and specialty product lines may require extended delivery schedules and will coordinate as needed.

4. Delivery Address

The vendor shall deliver all items to Wright-Patterson Air Force Base in Ohio.