D_Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: This request is for the purchase of a DELTA V Plus

Isotope Ratio Mass Spectrometer (IRMS) with a EA-Isolink for the purpose of measuring stable isotope ratios that may serve as useful biomarkers in nutritional epidemiology and creating cutting-edge research for understanding the influence of diet on aging and diseases. The IRMS has been proven to work well in research settings, in particular, for Baltimore Longitudinal Study of Aging (BLSA) study samples and results are currently run on a Delta V Advantage IRMS with EA and used in the continuity of current research sample and results with the intent of complementing existing data and future research needs.

2. Background Information and Objective: The National Institute on Aging (NIA), Intramural

Research Program (IRP), Translational Gerontology Branch (TGB) needs a DELTA V Plus IRMS with an EA-Isolink to conduct research. This instrument will be useful to a wide range of studies within NIA/IRP as related to epidemiological studies such as BLSA; Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing (GESTALT); clinical intervention studies such as the Ketone Study; animal studies; and even in vitro studies.

Stable Isotope Ratios are not affected by alterations to molecular structure, such as autoclaving or freeze/thaw issues. Therefore, stored samples from more than 50 years ago (such as those historic BLSA samples), can be analyzed without issues. BLSA samples have been run on the DELTA V Advantage IRMS with EA, as a result we would like to continue collection of data to complement the existing data. In addition, this machine is the gold standard for measuring stable isotopes and is being used by other international experts in this field. Therefore, results from our future studies can be easily compared to others.

3. Generic Name of Product: DELTA V Plus Isotope Ratio Mass Spectrometer (IRMS) with an EA-Isolink

4. Purchase Description: DELTA V Plus Isotope Ratio Mass Spectrometer (IRMS) with a EA-

Isolink shall include a universal triple collector, Hydrogen Cup (HD) collector, computer package with ISODAT software suite with Flash IRMS elemental analyzer for Elemental Analyzer (EA) Isolink Carbon (C), Nitrogen (N), Sulfur (S), Oxygen (O), and Hydrogen (H) with single reactor for C,N,S. AS 1310 Autosampler with tray for 105 samples for EA Isolink.

Vendors must be able to provide these specifications:

• 3kV acceleration voltage

• 1-96 amu range @ constant 3kV

• Mass Analyzer entrance slit 200 μm

• Mass Resolution (@ 10% valley separation) ≥ 110 m/Δm (CNOS)

• Effective magnetic deflection Radius 191 mm

• Fore vacuum Pump is contained within chassis to maintain a noise level at or below

48 dB

• Continuous flow sample consumption of 0.06 nmol/s CO2 generates 1.5 V (5 nA) on m/z 44

• Continuous flow Sensitivity of 1000 molecules per ion (m/z 44 on CO2)

• High sensitivity electron impact ion source self-aligning and plugs into a front flange of an analyzer block

• Dual Inlet and He carrier gas Continuous Flow applications

• Filament for all gas species

• Gas introduced to the ion source at ground potential

• Instrument diagnostics via software suite

• Differential pumping for less ion beam scattering in the fight tube

• Amplifier housing is held under vacuum

• Amplifiers register up to 50 V

• Computer switchable amplifier gain

• "Plug and measure" peripheral recognition

• Evacuated amplifier housing for lowest amplifier noise

• All analog digital conversion happens already in the amplifier housing under vacuum

• Computer controlled ion source potentials and parameters (fully integrated into the analytical process)

• Diagnostic package built into the software

• Equal treatment of all peaks in software (sample and reference)

Features of the ConFlo IV

• Five reference gases always available

• Computer-controlled reference gas intensities

• Auto-determination of linearity, stability, and H3+ factor

• Integrated system monitoring and self-diagnosis

• Computer-controlled auto-dilution of sample gases based on TCD signals from the

Flash EA

• an automated method developer that automatically matches reference peak height and sample peak height during the analysis

• Continuous and unattended analysis utilizing 3 preparation devices

• Software controlled standby and startup of Flash EA

• 2 high flow (EA) and 1 low flow (GC) gas inputs; automatically selectable from the computer

• An independent furnace operating up-to 1100oC for N, C and S analysis, with a 15-year parts warranty

• Ramp EA oven, the EA oven that can go from 60-240oC that can increase throughput and accuracy

• An independent Furnace operating up-to 1450oC for O and H analysis

• A Helium Management module (HeM) that delivers very low helium consumption for each sample analyzed (patented)

• Temperature regulated electronic flow controllers of helium carrier/reference gas and oxygen gas

• A Thermal Conductivity Detector, in a thermo-regulated environment, with a 15-year parts warranty

• Two MAS Plus autosamplers each supplied with one 32-position sample tray

• An automated switching device between C,N,S and O,H mode

• Automated wake-up, start-up, auto-off, auto-Standby, Leak Test and safety cut-off function

• Data Handling Software

• Flash IRMS Elemental Analyzer (EA) fully integrated with a software suite

• Automated standby mode with zero helium consumption

• Sequence delay timer for sample throughput management during maintenance periods

• Auto-stop function

5. Salient characteristics: N/A

6. Quantity: One (1)

7. Delivery Date: Ninety (90) days after receipt of completed purchase order

8. Period of Performance: N/A

9. Option(s): N/A