D_Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Institute of Aging (NIA) requires the acquisition is to purchase the Chromium Next GEM single cell Multiome ATAC and Gene expression kits.

2. Background Information and Objective:

The differences between individual cells can have profound functional consequences, in both unicellular and multicellular organisms. Developed single-cell ATAC-sequencing and single cell multimome ATAC+Gene Epression methods enable unbiased, high-throughput, and high-resolution epigenomic and transcriptomic analysis of individual cells. This provides an additional dimension to transcriptomic and epigenomic information relative to traditional methods that profile bulk populations of cells. Single-cell Multiome profiling of the transcriptome and epigenome at single cell resolution can transform the researchers understanding of biology, enhance the characterization of cell types and states, and gain deeper insights into underlying gene regulatory mechanisms with two readouts from every cell. Single Cell Multiome ATAC + Gene Expression and multiply our power of discovery. These single cell sequencing methods have revealed new biology in terms of the composition of tissues, the dynamics of transcription, and the regulatory relationships between genes. Rapid technological developments at the level of cell capture, phenotyping, molecular biology, and bioinformatics promise an exciting future with numerous biological and medical applications.

3. Generic Name of Product: Single cell Multiome ATAC + Gene Expression kits

4. Purchase Description: Chromium Next GEM Singl Cell Multiome ATAC+Gene Expression Reagent

Bundle, 16 rxns cat. # 1000283.

5. Salient characteristics:

a. Simultaneously profile gene expression and chromatin landscape from the same cell

b. Efficiently partition 500- 10,000 nuclei per channel, for up to 80,000 nuclei per run

c. Low microfluidic multiplet rate of <0.1% per 1,000 nuclei.

d. High sensitivity; similar performance to standalone Single Cell ATAC or Single Cell Gene

Expression Solutions performed on nuclei

e. Achieve deep characterization of cell types and states with linked transcriptomic and epigenomic analyses

f. Easy-to-use software for data analysis and visualization

g. Must be compatible with NIA 10X Chromium Connect automated instrument.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

6. Quantity: 4

Item Description (or equal) Catalog No. Quantity

Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns

1000283 4.00

7. Delivery Date: Within 30 days after contract award.

Delivery Location:

National Institute on Aging BRC building, Room 6C211A 251 Bayview Boulevard Baltimore, MD 21224.