CN-770000-16074-TA 20201.pdf

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Master Agreement FL|16074|369e4ae42feb2536e91a08e81a59e009
Contract number
16074
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Florida

About this file

This document is a Task Assignment Notification Form and Multi-Year Project Request from the Florida Fish and Wildlife Conservation Commission (FFWCC) awarded to the University of Georgia Research Foundation for a research project investigating Avian Vacuolar Myelinopathy (AVM) in Florida. The contract (FWC Contract No. 16074) involves researchers from the University of Georgia's Warnell School of Forestry and Natural Resources, with primary investigators including Susan B. Wilde and Mike Netherland from the Center for Invasive and Aquatic Plants.

The project focuses on researching the ecological risks associated with Aetokthonos hydrillicola, a cyanobacteria linked to mortality in freshwater vertebrates, particularly its association with the invasive aquatic plant Hydrilla verticillata. The research aims to understand the bacteria's distribution, toxicity, and impact on wildlife, especially snail kites and bald eagles. The contract is structured as a fixed-price agreement with a total task assignment value of $20,000, covering a three-year period from 2018 to 2020. Research objectives include sampling various lakes, evaluating toxin concentrations, creating site-specific and regional risk assessment models, and developing management recommendations for water resource and wildlife managers in Florida.

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Text version

FFWCC Multi Year Project Request

Date: April 30, 2018

Project Title:

Investigating risk and management of Avian Vacuolar Myelinopathy in Florida

Contact Information:

Need for research and outreach:

Aetokthonos hydrillicola Ah

Hydrilla verticillata Ah Hydrilla

Ah Hydrilla Haliaeetus leucocephalus

Ah

Ah Hydrilla

Ah Ah

Ah

Ah

Ah Ah

Ah

Ah

Research Efforts and Results:

1. Continue hydrilla and other SAV sampling from known Ah positive sites including

Tohopekeliga, East Lake Toho, Hatchineha, Cypress, Kissimmee, Istokpoga, and Ah negative sites including Okeechobee, and Stormwater Treatment Areas to assess water quality, water chemistry, seasonal levels of Hydrilla/Aetokthonos hydrillicola, and AVM toxin

2. Evaluate samples using remote sensing, microscopy, Zebrafish and HPLC

3. Determine locations at highest risk for AVM disease using density of Hydrilla/Ah and concentration of AVM toxin

4. Work with FFWCC, UF, and Audubon personnel to process and submit waterfowl, eagles, and snail kites for AVM histological analysis

5. Create site specific models of AVM risk assessment based on prevalence of hydrilla, water/sediment quality, A. hydrillicola, AVM toxin concentration and bird health.

A. hydrillicola

Objective 1: Seasonal and spatial analysis of A. hydrillicola and AVM toxin in Toho and additional lakes.

A. hydrillicola

A. hydrillicola

Aetokthonos hydrillicola

Evaluate samples using remote sensing, microscopy, PCR, and HPLC

Aetokthonos hydrillicola

Objective 3: Determine locations at highest risk for AVM disease using density of A.

hydrillicola and concentration of AVM toxin. A. hydrillicola

Aetokthonos hydrillicola

Objective 4: Create site specific models of AVM risk assessment based on prevalence of hydrilla, A. hydrillicola, AVM toxin concentration and bird health. A.

hydrillicola

Deliverables:

Schedule:

Continue hydrilla sampling from Lake Tohopekeliga and initiate additional lake SAV sampling from assess seasonal levels of AVM toxin Evaluate samples using microscopy, bioassays, and HPLC Determine locations at highest risk for AVM disease using density of A. hydrillicola and concentration of AVM toxin Create site specific models of AVM risk assessment based on prevalence of hydrilla, A.

hydrillicola, AVM toxin concentration and bird health.

Continue hydrilla sampling from Toho and additional lakes Evaluate samples using microscopy, bioassays, and HPLC Create site specific model of AVM risk assessment based on prevalence of hydrilla, A.

hydrillicola, AVM toxin concentration and bird health.

Create a regional AVM risk model incorporating water quality, sediment class, connectivity, and landscape level data.

Publications citing FFWCC support during 2016 18:

Rosthramus sociabilis.

doi:

Avian Vacuolar Myelinopathy

Presentations citing FFWCC support during 2016 2018:

Hydrilla verticillata

Total Salary $12,291

Total fringe: $1,697

Total supplies: $1,533.82

A. hydrillicola

Total travel: $1,200.00

Total Misc: $1,460.00

Total Budget: $20,000

Summary Proposed Budget Year 3

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