B. Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

Acquisition of a Tecan Fluent 1080, Infinite Microplate Reader, and a TeVacS for the Drug Metabolism & Pharmacokinetic (DMPK) Core at National Center for Advancing Translational Sciences (NCATS)

2. Background Information and Objective:

NCATS Drug Metabolism & Pharmacokinetic (DMPK) Core requires a Tecan Fluent in the main laboratory. The new Tecan Fluent platform will enhance the high throughput screening capabilities for not only the DMPK but for all of NCATS when screening compounds for Tier I ADME properties. The Tecan Fluent platform will be able to accommodate up to 6 plates on the bench top depth of the instrument. The system will offer one touch easy teaching for third party devices.

Attached to the new Tecan Fluent will be the Liquid Handling Arm (LHA) and Robotic Manipulating Arm (RMA) which will allow the system to transfer volumes down to 200nL and dispense different volumes simultaneously. This will save resources and time when running experiments. The Infinite Microplate Reader which will be attached to the new Tecan Fluent will be able to detect UV-Vis and fluorescence which we use in our DMPK laboratory daily when screening assays. Overall, the Tecan Fluent platform software will provide a dedicated operator interface for the easy daily running of the system. In addition, the Infinite Microplate Reader instrument software will provide luminescence and fluorescence-based detection, which is another highly used detection modality.

3. Generic Name of Product:

Tecan Fluent 1080

4. Purchase Description:

Item Product# Description Qty

Fluent 1080 AirFCA, MCA, with RGA 1 30042031 Fluent 1080 Base Unit, includes 1 year warranty 1 2 30042032 Full Door Panel, Fluent 1080 1 3 30042664 Door Lock Set, Fluent (1 pair) (for front shield) 1 4 30042582 Panel Set Base, Fluent 1080 1 5 30042588 Panel Set Top, Long Z, Fluent 1080 1 6 30042594 Top Cover for Std & Long Z RGA, Fluent 1080 1 7 30042715 Grid Segment, Extension, Rear, 20 Grids, New Instrument Only 4

Arm #1 AirFCA 8 30042200 Flexible Channel Arm (FCA), Air, 8-Tip 1

Arm #2 AirMCA

9 30042350 Multi-Channel Arm(MCA), Fluent 1 10 30051709 Head Adapter Set, includes 30032060 and 30032062 1 11 30032027 Rack, Head Adapter, MCA 384 2 12 30041305 Deck Segment, MCA384, Diti Active Carrier, Front 1

Arm #3 RGA

13 30042405 Robotic Gripper Arm (RGA), Extended z-rail 1 14 30042430 RGA Fingers, Eccentric, Standard, set of 2 1

Fluent 1080 Cabinet

15 30042035 Cabinet, Fluent 1080 1 16 30042054 Shelf, Cabinet, for Devices, Large 1 17 30042074 Door Lock Set, Fluent Cabinet (1 per door) 4 18 30149966 Mounting bracket for monitor arm 1

19 30100539 Ergotron 200 series Combo Arm (monitor + keyboard/mouse), black color, for EVO or Fluent 1

Deck Segments Included

20 30042712 Grid Segment, Side Wall, Left, 87mm 1 21 30042524 Deck Segment, Troughs, 100 mL, 8-Pos, with Waste Chute 1

22 30042738 Nest Segment, DiTi Waste Chute Through Deck, 4-Position, Landscape, 7mm 1

23 30126013 Grid Segment. 6 Grids, Multipurpose 1 24 30042725 Nest Segment, 6-Position, Landscape, 7 mm 7 25 30042781 Nest, for Fluent Deck Segment, Microplate/DiTi, 61mm 6

26 30187662 SHAKER QINTRSUMENTS BIOSHAKE Q1 3

27 30127737 Microplate Adapter, Flat, Q.Intsruments BioShake 3000, Standard, 2016 3

28 30136963 Driver, Q.Instruments, BioShake 3000, EVOware and Fluent Control 1

29 30214873 Edgeport (Digi) 4-port serial adapter, converts one USB port to 4 RS-232 serial ports. 1

30 30042863 Hotel, Fluent, 9-Position, Microplates or Tip Boxes, Adjustable Shelves 3

31 30042711 Grid Segment, Side Wall, Right, 87mm 1

Te-VacS

32 10760680 Vacuum Separation, High Throughput, Te-VacS 1 33 30042701 Grid Segment, 6 Grids 1

34 10760680 Base Plate, Te-VacS 1

35 10760626 Vacuum Block, Type C, Tall, Clear, Biology, for Top Skirted Cartridges (Qiagen), Te-VacS 1

36 30042799 Grid Adapter Set for Optional Devices, EVO Style Grids, Set of 2 1

Infinite Microplate Reader (UV-Vis/Fluorescence)

Computer and Accessories

37 30216478 Computer, Desktop, Windows 10 Professional 1 38 30123658 Monitor, Touchscreen, 22” LCD, Planar 1 39 30024944 Keyboard and Mouse, Wireless 1

Training

40 30216156 FluentControl (US) 4 Days 1

Installation and Software Overview

41 30181008 Introspect 1 year license, included at point of sale with new instrument purchase only 1

42 30042001 Fluent Gx Assurance Package 1

43 30001582 Installation and Software Overview, for Hardware installation (new and upgrade) 1

5. Salient Characteristics:

This equipment must:

Fluent General Platform

• The system must be able to accommodate up to 6 plates in the bench top depth of the instrument.

• System must be teach free for standard microplate modules.

• System must offer one touch easy teaching for third party devices.

• The system must offer a tailored and customizable run time environment separate from the setup software.

• The system must have an integrated controlled e.g. touch screen for daily operation/ operation without keyboard.

Liquid Handling Arm and Robotic Manipulating Arm

• System must be able to transfer volumes down to 200nl.

• Each tip must be able to dispense a different volume at the same time.

• Each pipetting channel must be able to individually access samples, one at a time.

• The disposable tip system must be able to offer nested tips for increased capacity and to dispose of the nesting wafers without use of gripper arm.

• High pipetting security must be available through sensitive and adaptive liquid level detection.

The system must recognize no liquid , not enough liquid, incorrect aspiration with a single liquid level detection and aspiration supervision event generating dual and confirmatory signals

• System must include an eight-tip liquid handling arm with variable distance between tips from 9 to 38mm. Each tip must be independently controlled and be used individually to access samples, one at a time.

• Pipetting in each tip of the 8 tips must be controlled with a precision motor which drives a plunger resulting in air displacement to enable sample pipetting.

• Pipetting system must NOT be driven by a liquid filled circuit activated by syringes. For simplified maintenance purposes, no liquid filled pipetting, no system fluid and waste bottles required.

Water displacement technologies will not be considered because of contamination potential which is deleterious to RNA extraction protocols.

• Pipetting arm internal channels must be protected by an in-line filter.

• Pipetting technology must include;

o cLLD – capacitive Liquid Level Detection for use with conductive liquids o AST – Adaptive Signal Technology

• The instrument must utilize disposable tips. All tip sizes must utilize the same disposable tip adapter. Disposable tips are available in 10, 50, 200 and 1000 µL sizes.

• Disposables tips are available from the original manufacturer AND 3rd party plasticware suppliers. Tips must NOT be restricted to a patented design. Disposable tips for the system must be available from at least 3 DIFFERENT manufacturing sources.

• The instrument must have a pipetting range from 0.5uL to 1000ul.

• All liquid handling parameters, such as air gap sizes, aspiration and dispense speeds and mixing must be accessible to the operator and must be easily accessible for change.

• The system must be able to utilize up to two independent arms for greater throughput through multitasking. A Liquid Handling arm as well as an independent robotic gripper arm to move labware and consumables around the work surface.

• For any reason, there must be the possibility to easily upgrade or exchange the arms of the robotic system at the customer’s site.

• System must be enclosed with Plexiglas protection shields, securing the work deck and user accidental tampering.

• The Robotic gripper should have access to the edges (side, front and rear) of the system to allow optimal device integration and minimize occupation of the liquid handling area.

• Operator must be able to stop the operation of the instrument by opening the front shield.

Software

• The system must provide a dedicated operator interface for the easy daily running of the system. The system will preferably offer a touch interface rather than keyboard and mouse.

• The system must allow support less experienced users to set up the system - such as embedded usage guides, SOPs, photos or videos

• Editing of the software protocol must be able to be navigated from a single program interface.

• System software is equipped with an instrument simulator that can be installed on many separate PC in order to program entire functional methods at a remote location while the actual instrument is running.

• The virtual simulator must visually reproduce instrument movements and respond with the same return commands as the actual instrument.

Accessories, Storage and Miscellaneous

• The system must be able to accommodate single tube-racks for microfuge tubes in an arrangement of 1x32 tubes.

• Instrument must have an open-deck arrangement where individual labware carriers are removable.

• Instrument must enable the possibility of carrier mounting to a lower surface below the primary worktable surface for form a “lower deck”.

• The system must have a front-mounted touchscreen where a Graphical User Interface may be used to run the method. This front-mounted touchscreen must be separate from the monitor used with the instrument control computer.

• The system must enable customized Graphical User Interfaces for showing run setup, inputting settings such as sample count, volume, number of plates, etc. into the script, as well as enable selection of worklist files from a Windows directory.

Integrated Peripherals

• The system can be integrated with a fully automated multimode microplate reader which includes Absorbance and Fluorescence.

• The system must include a shaking device with variable orbital settings and software-controlled speed.

• The system must have 3 cooling devices (+4C to 70C) critical for keeping precious reagents at 4C and enabling complex protocol incubations.

6. Quantity: One (1) configured as shown in Purchase Description

7. Delivery Date: Approximately 4-6 Weeks ARO

8. Period of Performance: One time delivery

9. Option(s): N/A