Attachment 1-Requirements Document.docx

STATEMENT OF WORK/REQUIREMENTS DOCUMENT

Title: Supercontinuum light source with filters Requesting Lab: PML

I. BACKGROUND INFORMATION

Purpose/Objective: The objective of the gene therapy manufacturing project is to develop new methods to better characterize gene therapy particles at high throughputs. Emerging gene therapy treatments use gene delivery nanoparticles, such as viral vectors and non-viral carriers, to cure intractable diseases. These manufactured particles suffer heterogeneity issues that hinder treatment efficacy and safety. This project aims to develop light-scattering microscopy methods to enable rapid, multi-attribute measurements of individual gene therapy nanoparticles’ physical characteristics. Such measurements will speed the development of new gene therapy treatments and improve their efficacy and safety. The supercontinuum light source and tunable filters are required to meet this objective.

II. SCOPE

The Contractor shall deliver a quantity of one (1) of a broadband, high power supercontinuum light source, quantity one (1) of a wide bandwidth tunable filter with single-mode fiber output, and quantity one (1) of a multiline, narrow bandwidth, fast switching tunable filter with single-mode fiber output, inclusive of FOB Destination delivery, warranty, necessary accessories to integrate the filters with the light source, and necessary drivers and software to control the filters and light source via a computer.

III. MINIMUM REQUIREMENTS

The Contractor shall provide a system that meets all technical specifications identified below. All items must be new. Used or remanufactured equipment will not be considered for award. Experimental, prototype, or custom items will not be considered. The use of “gray market” components are not authorized for sale in the U.S. by the Contractor is not acceptable. All line items shall be shipped in the original manufacturer’s packaging and include all original documentation and software, when applicable.

Line Item 0001: Super continuum laser Description: The Contractor shall provide the following VIS-NIR supercontinuum light source that meets the following minimum specifications. This item is also known as a white light laser.

Quantity: 1 A. Technical Specifications

1. The supercontinuum light source shall have a broadband spectral output covering the wavelength range of 420 nm to 1100 nm or broader.

2. The output of the light source shall have a single spatial mode throughout the 420 nm to 1100 nm spectral region or broader.

3. The time-averaged continuous spectral power density output shall be greater than or equal to 1 mW/nm throughout the 450 nm to 500 nm wavelength range and greater than or equal to 2 mW/nm throughout the 500 nm to 1100 nm wavelength range. The required power densities are necessary to achieve fast frame rates and large fields of view for the microscopy apparatus.

4. After an initial warm up time, the output power shall have a nominal stability of 1% or smaller.

5. The light output shall be collimated with a beam diameter no larger than 5 mm throughout the 420 nm to 1100 nm spectral region.

6. The light output shall be directly compatible with both tunable filters mentioned below (Line Items 0002 and 0003), with no significant alignment steps required.

7. The supercontinuum light source shall be controllable both with and without a computer.

8. The supercontinuum light source shall operate on 120 VAC for all components.

9. The supercontinuum light source shall include all necessary power supplies, cables, adapters, etc. for normal operation.

10. An installation and user manual shall be included with the supercontinuum light source which fully describes proper set-up, installation, and usage.

Line Item 0002: Wide bandwidth tunable filter Description: The Contractor shall provide the following tunable filter that meets the following minimum specifications. Bandpass filters capable of meeting these specifications typically operate by making use of short-pass and long-pass linear variable filters.

Quantity: 1 A. Technical Specifications

1. The filter must be directly compatible with the supercontinuum light source above (line item 0001) with no significant alignment steps required. For laser safety, there must be no free space laser beam between the light source and output of the filter.

2. The filter shall have a spectral range of 420 nm to 800 nm or broader.

3. Across the entire 420 nm to 800 nm spectral range, the minimum bandwidth achievable must be 10 nm or smaller with a transmission of 60% or greater.

4. The maximum bandwidth achievable must be the entire 430 nm to 800 nm spectral range, or greater.

5. Light at wavelengths longer than 1100 nm must be blocked by the filter with an optical density of 3 or greater.

6. Wavelength tuning must take no more than 2 s for a 10 nm step.

7. After an initial warm up time, the output power shall have a nominal stability of 1% or smaller.

8. The filter must maintain the single-spatial mode of the input light source.

9. The filter must maintain the polarization state of the input light source (typically unpolarized).

10. The output must be reconfigurable by NIST to allow free-space output of the collimated beam, coupling into a single-mode optical fiber, and coupling into a multimode optical fiber. Attaching different accessories and minimal alignment steps are acceptable when switching output configurations, but these accessories must be included.

11. The system must come with a single-mode optical fiber (non-polarization maintaining) that maintains single-mode characteristics over the entire 420 nm to 800 nm spectral range. The fiber length shall be 2 m or greater.

12. The single-mode optical fiber must have FC/APC termination.

13. The filter shall be controllable with a computer.

14. Software shall be included to operate the filter by a stand-alone graphical user interface, and by a software development kit with libraries in the programming language C, Python, Matlab, or similar.

15. The filter shall operate on 120 VAC for all components.

16. The filter shall include all necessary power supplies, cables, adapters, etc. for normal operation.

17. An installation and user manual shall be included with the filter which fully describes proper set-up, installation, and usage.

Line Item 0003: Multiline, fast-switching wavelength filter Description: The Contractor shall provide the following tunable filter that meets the following minimum specifications. Bandpass filters capable of meeting these specifications typically operate by making use of acousto-optic tunable filters in order to have the short switching time required.

Quantity: 1 A. Technical Specifications

1. The filter must be directly compatible with the supercontinuum light source above (line item 0001) with no significant alignment steps required. For laser safety, there must be no free space laser beam between the light source and output of the filter.

2. The filter shall have a spectral range of 420 nm to 1100 nm or broader. Up to two separate acousto-optic crystals may be used to cover this spectral range. A wavelength gap of no more than 50 nm is acceptable between crystals.

3. At least 8, separate and individually tunable filter lines must be usable.

4. If two crystals are used, it must be possible to switch between the two at least as fast as the switching time specified in specification 7 below.

5. Across the entire 420 nm to 1100 nm spectral range, the minimum bandwidth of each line must be 8 nm or smaller with a transmission of 60% or greater.

6. Light at wavelengths longer than 1100 nm must be blocked by the filter with an optical density of 3 or greater.

7. Changing the position of a line and turning a line off or on must take no more than 0.1 ms. This fast switching time is necessary to cycle wavelengths in the microscopy apparatus while recording videos at frame rates exceeding 1000 frames per second.

8. After an initial warm up time, the output power shall have a nominal stability of 1% or smaller.

9. The filter must maintain the single-spatial mode of the input light source.

10. The output must be reconfigurable by NIST to allow free-space output of the collimated beam, coupling into a single-mode optical fiber, and coupling into a multimode optical fiber. Attaching different accessories and minimal alignment steps are acceptable when switching output configurations, but these accessories must be included. Note that if two outputs are provided, one for each acousto-optic crystal, these options must be available for both outputs.

11. The system must come with a polarization-maintaining single-mode optical fiber that maintains single-mode characteristics over the entire 420 nm to 1100 nm spectral range. The fiber length shall be 2 m or greater. Note that if two outputs are provided, one for each acousto-optic crystal, two-such fibers are required.

12. The polarization-maintaining single-mode optical fiber(s) must have FC/APC termination.

13. The filter shall be controllable with a computer.

14. Software shall be included to operate the filter by a stand-alone graphical user interface, and by a software development kit with libraries in the programming language C, Python, Matlab, or similar.

15. The filter shall operate on 120 VAC for all components.

16. The filter shall include all necessary power supplies, cables, adapters, etc. for normal operation.

17. An installation and user manual shall be included with the filter which fully describes proper set-up, installation, and usage.

IV. DELIVERABLES

V. PLACE OF PERFORMANCE

National Institute of Standards and Technology Building 217 Room G122 100 Bureau Dr.

Gaithersburg, MD 20899

VI. PERIOD OF PERFORMANCE/LEAD TIME

6 weeks ARO

VII. DELIVERY TERMS

Delivery shall be F.O.B Destination and shall occur in accordance with the delivery due dates provided in the above table.

FOB Destination means: The contractor shall pack and mark the shipment in conformance with carrier requirements, deliver the shipment in good order and condition to the point of delivery specified in the purchase order, be responsible for any loss of and/or damage to the goods occurring before receipt and acceptance of the shipment by the consignee at the delivery point specified in the purchase order; and pay all charges to the specified point of delivery. The contractor shall deliver all Line Items to:

National Institute of Standards and Technology Shipping and Receiving 100 Bureau Drive, Building 301 Gaithersburg, MD 20899 Aaron Goldfain. Building 220, Room A317.

VIII. INSPECTION & ACCEPTANCE

In addition to the inspection and acceptance terms articulated in 52.212-4, the Government reserves the right to perform such performance tests and evaluations as defined below to verify specified system performance. Such tests and evaluations, if performed, shall be conducted within the environment that the system is to be operated. The Contractor has the right to be present during the tests and evaluations, if performed, at the Contractor’s expense.

Performance Tests:

1. Visual inspection

a. General inspection of the instrument and components to ensure everything has been included and there is no damage.

b. The instrument will be operated using manufacturer recommended procedure.

2. Test the performance of the supercontinuum laser (item 0001) when used with the wide bandwidth tunable filter (item 0002).

a. Measure the power output as a function of wavelength with the bandwidth set to 10 nm.

b. Verify that the specified bandwidths are accessible (see requirements 3 and 4 for line item 0002 above).

c. Measure the output power stability.

d. Measure the time required to change the filter wavelength by a 10 nm step.

3. Test the performance of the supercontinuum laser (item 0001) when used with the multiline, fast switching bandwidth tunable filter (item 0003).

a. Measure the output power over the 420 nm to 1100 nm spectral range for a single output line.

b. Verify that 8 bands can be used simultaneously.

c. Measure the output power stability.

d. Measure the time required to change the line wavelength and turn on and off line wavelengths.

NIST may choose at its discretion to forego this part of acceptance testing.

A visual inspection of the equipment will be performed by the NIST TPOC to identify surface defects or any form of indication that any equipment was damaged during transport to NIST. The Government shall have sole discretion to require repair or replacement of damaged and/or nonconforming supplies at no cost to the Government. The Government at any time prior to acceptance shall reject the equipment due to defects and/or nonconformance. The vendor is responsible for latent defects discovered any time after final inspection. However, the extent of its liability shall be prorated over the useful life of the equipment.

Ownership of the equipment shall transfer to NIST upon acceptance by the Government.

The Government will test, inspect, and accept or reject the equipment within 30 working days of the receipt of the equipment unless otherwise indicated above. The Government reserves the right to conduct quality assurance testing to confirm that a given instrument(s) meets the manufacturer’s and/or the Government’s performance specifications. It is anticipated that the equipment will meet all manufacturer’s specifications and/or the Government’s performance specifications identified in the most recent operations and maintenance manual for each piece of equipment and/or in this document.

IX. WARRANTY

The contractor shall warranty the entire system for a period of a minimum of two (2) years after receipt of the equipment and shall be in accordance with terms in FAR 52.212-4. Warranty shall commence upon acceptance of the system by the Government and at a minimum shall include the following: coverage of the supercontinuum light source and both tunable filters.

X. PAYMENT SCHEDULE

Advance payment is not authorized. The Contractor must invoice in arrears according to the payment schedule. The Contractor shall be paid, in accordance with Net 30-day payment terms, upon receipt and acceptance of a proper invoice, in accordance with the following schedule:

1. 100% after installation and acceptance by the TPOC of fully installed system, AND

1. After the successful completion of the testing requirements set forth in this document under section set forth in this document, AND

1. After successful demonstration by the instrumentation that it performs IAW the technical requirements set forth in this document AND

1. After receiving an invoice submitted properly, IAW the purchase order terms and conditions.

NOTE: Partial shipments and partial invoices will not be accepted, unless otherwise requested and accepted by the Contracting Officer prior to award offer. Proposed payment schedules shall be submitted with vendor’s response to the RFQ for consideration.