Attachment 1 - Purchase Description_Zeiss 900-980 scanning system or equal.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Title: Zeiss LSM900 and LSM980 system or Equal

1. Statement of Need and Purpose: The purpose of this acquisition is to procure laser point-scanning confocal microscope systems designed for imaging stained and live tissue. The confocal laser microscope will be used to image Drosophila brain and other tissue stained with multiple antibodies with/or fluorescent in situ probes. The primary purpose is to be able to heavily multiplex different fluorophores on the same brain/ tissue and take high-quality images to find out dendrite morphology change. The multi-photon scanning system will be used to take live imaging of Drosophila brain with a fluorophore probe. With our current multi-photon laser turned to 920nm, the system has a wide range of detection, which can simultaneously scan and collect fluorophore movement in the live brain. The collected data will be used for the analysis of dynamic change at the early dendrite development stage.

2. Background Information and Objective:

Dendrite Morphogenesis and Plasticity Section( DMPU) is a section of NIH/NINDS.

Applying the Drosophila larval visual circuit, DMPU investigates the structural and functional plasticity change to find the mechanisms underlying the neurolonal development. We need to perform large-scale genetic screens on drosophila with a confocal system to take high-quality images to determine the dendrite morphology change at the cell level. Furthermore, analyze these candidate genes using in vivo live imaging with a multi-photon scanning system. The primary approach is understanding the molecular path to regulate dendrite morphogenesis and structural plasticity and learning the consequent function change due to deficits in homeostatic neuronal plasticity.The confocal laser microscope and multi-photon scanning system described above will allow the DMPU to screen large-scale genetically modified drosophila containing fluorescent reporters to learn certain gene functions and lively tracing fluorescent signal movement in the larva brain.

1. Generic Name of Product: confocal laser scanning microscope system and multi-photon scanning system

4. Purchase Description: One microscope system and one multiphoton scanning system

( including scanning stages, widefield fluorescence, transmitted light, objectives, LSM, supportive workstation and software)

Brand-name item: LSM900 and LSM980 system

Manufacturer: Carl Zeiss

5. Salient characteristics: : The vendor shall provide equipment brand name or equal to

LSM900 and LSM980 system with the following material characteristics:

Confocal scan head:

• Must have ability for multitrack imaging, no cross-talk between different channles, by linewise or framewise switching of the laser excitation line.

• Must have freely rotatable scan field (0-360 degree in 0.1° steps) and free xy offset (crop functionality).

• Must have up to 4 laser lines could be used simultaneously.

Available Lasers :

405 nm, solid state laser, 5 mW

488 nm, solid state laser, 10 mW

561 nm, solid state laser, 10 mW

639 nm, solid state laser, 5 mW

• Must have different scan speed levels (34/26 levels when including bi-directional scans).

512 x 512 pixels at max. 8 frames/sec

256 x 256 pixels at max. 13 frames/sec

512 x 16 pixels at max. 160 frames/sec

6513 lines/sec( multi-photon scanning system)

• Must have absolute linear scanner movement to ensure equal pixel dwell-times as a prerequisite for any quantitative study.

• Could variable scan field size (hardware-zoom) from 0.5 to 40 in 0.1 steps for magnifications up to and beyond the optical limits of resolution

• Must have PMT detectors with gain calibration and linearization.

• Could acquire multidimensional image with combinations of Z-Stack, Time Series, Multi-positions (including software based autofocus options)

• Must have reliable separation of different fluorescent dyes even with highly overlapping emission spectra.

Confocal microscope:

• must be fully motorized stage including: Motorized Z, Nosepiece, Port selection

• Must have following microscope objectives:

-EC Plan-Neofluar 10x/0.30 WD

-Objective PApo 40x/1.3 Oil DIC WD=0.20

-Objective C-Apo 40x/1.2 W Corr( for multi-photon scanning system)

• Must have wildfield fluorescence and transmitted light

• Must have Image format from 4x1 to 6k x 6k( Confocal laser microscopy systerm) pixels, and 4x1 to 8192x8192( multi-photon scanning system) pixels with up to

16 bit depth (65536 gray values) in all PMT channels.

multi-photon System

• Must have automatic bleach control in Time Series experiment to design the entire experimental protocol. All scan strategies can be applied (line, spline, frame, z-line, z-spline, Z-Stack, ROIs, z-ROIs) in the time series.

• Must have Multiple (up to 99) freely definable regions of interest (ROIs). Have pixel precise control of the laser intensity and signal detection. Prevention of photo damage outside the ROIs boundaries when work on living cells, FRET, FRAP, uncaging, photoactivation and photoconversion experiments

• Detector could acquire 9.7nm spectral resolution for a full spectral scan.

• The TwinGate main beamsplitter utilizing 2 separate 13 position MBS wheels allowing up to 50 different laser combinations while maintaining a typical laser suppression of OD 7 or greater.

• Must have optimum sensitivity to have evenly spectral dispersion over the entire visual spectral range (380-750 nm).

Workstation/Software:

• Have premium workstation to support confocal microscope working.

• Have fully motorized set-up of the optical path and is configured using ZEN software.

• The acquisition and analysis of spectral data is fully integrated in the basic software (ZEN).

• Spectral data can be stored in the spectral database and later used for reference-based linear unmixing.

• Could create customized workflow, Software programmability and automation with control over all image acquisition functions.

• Have Self-Maintenance Tool, an easy-to-use software along with a calibration objective for maintaining optimal system performance by automated calibration routines.

• Smart Setup: Easy hardware control - adapts system configuration according to chosen dyes from a large database of fluorochromes. Different acquisition modes are suggested (e.g. Fastest or Best Signal).

These features meet the government’s primary minimum requirement of having the ability to multiplex probes for confocal fluorescence imaging of fixed brain tissues, and acquire live tissue imaging.

6. Other Requirements: The vendor shall provide an Original Equipment Manufacturer

(OEM) certified technician to perform the setup and installation of the equipment, and provide the initial training.

7. Quantity: One microscope system and one multiphoton scanning system

8. Delivery, Installation, and Training Date: The Vendor shall deliver the equipment to

Building 35 at the National Institutes of Health in Bethesda, Maryland within 16 weeks days after receipt of order. The equipment shall be delivered and installed between the hours of 8:00am and 5:00pm EST, Monday through Friday. Delivery must be Freight on

Board (FOB) Destination and include inside delivery. The equipment shall be delivered and installed in coordination with the Contracting Officer’s Representative (COR) at the time the equipment is ready to be shipped.

The Vendor shall provide on-site training within 30 days after equipment installation.

9. Period of Performance: includes at least 1 year warranty on the system.